Structure of ligand-gated ion channels: Critical assessment of biochemical data supports novel topology

Molecular and Cellular Neuroscience

Volumn 17, Issue 5, 2001, Pages 777-792

  Leite, John F ^a,b   Cascio, Michael ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 AMINOBUTYRIC ACID RECEPTOR; GLYCINE RECEPTOR; ION CHANNEL; PROTEIN SUBUNIT;

ALPHA HELIX; AMINO TERMINAL SEQUENCE; CHANNEL GATING; HUMAN; MODEL; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; REVIEW; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION; SYNAPSE;

UNIDENTIFIED BACULOVIRUS;

EID: 0034986892     PISSN: 10447431     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcne.2001.0984     Document Type: Review

References (86)

1. An analog of lophotoxin reacts covalently with Tyr190 in the alpha-subunit of the nicotinic acetylcholine receptor
2. Identification of acetylcholine receptor channel-lining residues in the M1 segment of the α subunit
3. Identification of acetylcholine receptor channel-lining residues in the entire M2 segment of the α subunit
4. Reporter epitopes: A novel approach to examine transmembrane topology of integral membrane proteins applied to the a1 subunit of the nicotinic acetylcholine receptor
5. Topology of ligand binding sites on the nicotinic acetylcholine receptor
6. Topography of nicotinic acetylcholine receptor membrane-embedded domains
7. Ligand-gated ion channels in the brain: The amino acid receptor superfamily
8. Mapping the lipid-exposed regions fin the Torpedo californica nicotinic acetylcholine receptor
9. Identifying the lipid-protein interface of the Torpedo nicotinic acetylcholine receptor: Secondary structure implications
10. 3H]diazofluorene
11. A receptor: Evidence for a β-strand
12. Crystal structure of mouse acetylcholinesterase
13. β-barrel proteins from outer membranes: Structure, function and refoldfing
14. Functional expression and purification of a homomeric human α1 glycine receptor fin baculovirus-infected insect cells
15. Functional reconstitution and characterization of recombinant human a1 glycine receptors
16. The transmembrane topology of the amino terminus of the α subunit of the nicotinic acetylcholine receptor
17. Functional characterization of a potassium-selective prokaryotic glutamate receptor
18. Identification of amino acids contributing to high and low affinity d-tubocurarine sites in the Torpedo nicotinic acetylcholine receptor
19. Structure of the agonist-binding sites of the Torpedo nicotinic acetylcholine receptor: Affinity-labeling and mutational analyses identify γTyr-111/δArg-113 as antagonist affinity determinants
20. Multiple sequence alignment with hierarchical clustering
21. Nicotinic receptors at the amino acid level
22. Structure of the nicotinic receptor acetylcholine-binding site. Identification of acidic residues in the delta subunit within 0.9 nm of the 5 alpha subunit-binding
23. Post-synaptic inhibitory mechanisms of anaesthesia: Glycine receptors
24. Amino acids of the Torpedo marmorata acetylcholine receptor alpha subunit labeled by a photoaffinity ligand for the acetylcholine binding site
25. + conduction and selectivity
26. 3H]flunitrazepam is histidine 102 of the α subunit
27. Chimaeric nicotinic-serotonergic receptor combines distinct ligand binding and channel specificities
28. Structure of a glycerol-conducting channel and the basis for its selectivity
29. Allosteric transitions of the acetylcholine receptor probed at the amino acid level with a photolabile cholinergic ligand
30. Sequential roles of agrin, MuSK, and rapsyn during neuromuscular junction formation
31. The transmembrane domains of the nicotinic acetylcholine receptor contain α-helical and β structures
32. Identification of the alpha subunit half-cystine specifically labeled by an affinity reagent for the acetylcholine receptor binding site
33. Substituted-cysteine accessibility method
34. Conserved quaternary structure of ligand-gated ion channels: The postsynaptic glycine receptor is a pentamer
35. The ligand gated ion channel database
36. Improved secondary structure predictions for a nicotinic receptor subunit: Incorporation of solvent accessibility and experimental data into a two-dimensional representation
37. Coupled proteolytic and mass spectrometry studies indicate novel topology for the glycine receptor
38. Spatial structure of the M3 transmembrane segment of the nicotinic acetylcholine receptor alpha subunit
39. Photolabeling reveals the proximity if the α-neurotoxin binding site to the M2 helix of the ion channel in the nicotinic acetylcholine receptor
40. Pore loops: An emerging theme in ion channel structure
41. Specific binding sites for alcohols and anesthetics on ligand-gated ion channels
42. Identification of a gephyrin binding motif on the glycine receptor β subunit
43. 3H]Nicotine as an agonist photoaffinity label
44. Secondary structural analyses of the nicotinic acetylcholine receptor as a test of molecular models
45. A and glycine receptors
46. Nicotinic acetylcholine receptor at 4.6 Å resolution: Transverse tunnels in the channel wall
47. Proteolytic fragments of the nicotinic acetylcholine receptor identified by mass spectrometry: Implications for receptor topography
48. Ion channel modulation as the basis for general anesthesia
49. A receptor determined by electron microscopic image analysis
50. A receptors
51. Structures of the M2 channel-lining segments from nicotinic acetylcholine and NMDA receptors by NMR spectroscopy
52. Identification of a cytoplasmic region of the Torpedo nicotinic acetylcholine receptor α-subunit by epitope mapping
53. Identification of sites of incorporation in the nicotinic acetylcholine receptor of a photoactivatible general anesthetic
54. The synapse-associated protein rapsyn regulates tyrosine phosphorylation of proteins colocalized at nicotinic acetylcholine receptor clusters
55. Mutation of an arginine residue in the human glycine receptor transforms β-alanine and taurine from agonists into competitive antagonists
56. The unique extracellular disulfide loop of the glycine receptor is a principal ligand binding element
57. Transmembrane topography of nicotinic acetylcholine receptor: Immunochemical tests contradict theoretical predictions based on hydrophobicity profiles
58. A-receptor subunits in the rat retina
59. Functional expression in Xenopus oocytes of the strychnine binding 48 kD subunit of the glycine receptor
60. Mutation of glycine receptor subunit creates β-alanine receptor responsive to GABA
61. Expression and renaturation of the N-terminal extracellular domain of torpedo nicotinic acetylcholine receptor α-subunit
62. A model for the topology of excitatory amino acid transporters determined by the extracellular accessibility of substituted cysteines
63. Regulation of excitatory and inhibitory neurotransmitter-gated ion channels by protein phosphorylation
64. A receptor γ subunit
65. Probing the agonist domain of the nicotinic acetylcholine receptor by cysteine scanning mutagenesis reveals residues in proximity to the alpha-bungarotoxin binding site
66. Mapping the agonist site of the nicotinic acetylcholine receptor
67. Electron microscopic evidence for the assembly of soluble pentameric extracellular domains of the nicotinic acetylcholine receptor
68. Localization of the main immunogenic region of human muscle acetylcholine receptor to residues 67-76 of the alpha subunit
69. Acetylcholine receptor channel imaged in the open state
70. Nicotinic acetylcholine receptor at 9 Å resolution
71. Antagonism of ligand-gated ion channel receptors: Two domains of the glycine receptor alpha subunit form the strychnine-binding site
72. Structure and dynamics of the GABA binding pocket: A narrowing cleft that constricts during activation
73. Probing the structure of the nicotinic acetylcholine receptor with 4-benzoylbenzoylcholine, a novel photoaffinity competitive antagonist
74. The accessible surface of the nicotinic acetylcholine receptor
75. Water-soluble nicotinic acetylcholine receptor formed by α7 subunit extracellular domains
76. Expression and circular dichroism studies of the extracellular domain of the α subunit of the nicotinic acetylcholine receptor
77. γ-Aminobutyric acid increases the water accessibility of M3 membrane-spanning segment residues in γ-aminobutyric acid type A receptors
78. The location of the gate in the acetylcholine receptor channel
79. Acetylcholine receptor channel structure in the resting, open, and desensitized states probed with the substituted-cysteine-accessibility method
80. Unraveling the modular design of glutamate-gated ion channels
81. Amino acids lining the channel of the γ-aminobutyric acid type A receptor identified by cysteine substitution
82. A receptor by progressive deletions
83. A transmembrane site determines sensitivity of neuronal nicotinic acetylcholine receptors to general anesthetics
84. 3 receptor
85. Contribution of the β subunit M2 segment to the ion-conducting pathway of the acetylcholine receptor
86. From ab initio quantum mechanics to molecular neurobiology: A cation-pi binding site in the nicotinic receptor