Heat-induced unfolding of neocarzinostatin, a small all-β protein investigated by small-angle x-ray scattering

Journal of Molecular Biology

Volumn 308, Issue 4, 2001, Pages 721-743

  Perez J ^a   Vachette, P ^a   Russo, D ^b   Desmadril, M ^a   Durand, D ^a  

^a UNIVERSITÉ PARIS SACLAY   (France)

^b CEA SACLAY   (France)

Author keywords

Ensemble of conformations; Intermediate; Protein unfolding; Small angle scattering; sheet protein

Indexed keywords

ZINOSTATIN;

ARTICLE; BETA SHEET; DIFFERENTIAL SCANNING CALORIMETRY; HEATING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FOLDING; PROTEIN STRUCTURE; THERMODYNAMICS; X RAY ANALYSIS;

EID: 0034966446     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4611     Document Type: Article

References (88)

1. Kinetic studies of β-sheet protein folding
2. Long-range order in the src SH3 folding transition state
3. Mapping the transition state of the WW domain beta-sheet
4. Similarities between the spectrin SH3 domain denatured state and its folding transition state
5. Protein folding and unfolding on a complex energy landscape
6. Denaturant-induced movement of the transition state of protein folding revealed by high-pressure stopped-flow measurements
7. Experiment and theory highlight role of native state topology in SH3 folding
8. The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved
9. Two proteins with the same structure respond very differently to mutation: The role of plasticity in protein stability
10. The folding of an immunoglobulin-like Greek key protein is defined by a common-core nucleus and regions constrained by topology
11. Conservation of folding and stability within a protein family: The tyrosine corner as an evolutionary cul-de-sac
12. Folding of beta-sandwich proteins: Three-state transition of a fibronectin type III module
13. Folding studies of immunoglobulin-like beta-sandwich proteins suggest that they share a common folding pathway
14. Folding and stability of a fibronectin type III domain of human tenascin
15. The seven-stranded beta-barrel structure of apo-neocarzinostatin as compared to the immunoglobulin domain
16. Three-dimensional solution structure of apo-neocarzinostatin from Streptomyces carzinostaticus determined by NMR spectroscopy
17. A revised primary structure for neocarzinostatin based on fast atom bombardment and gas chromatographic-mass spectrometry
18. Neocarzinostatin: Spectral characterization and separation of a non-protein chromophore
19. Poly(deoxyadenylic-deoxythymidylic acid) damage by radiolytically activated neocarzinostatin
20. Binding of the nonprotein chromophore of neocarzinostatin to deoxyribonucleic acid
21. Characterization of the denatured states distribution of neocarzinostatin by small-angle neutron scattering and differential scanning calorimetry
22. Apparent radii of the native, stable intermediates and unfolded conformers of the alpha-subunit of tryptophan synthase from E. coli, a TIM barrel protein
23. Structural characterization of the molten globule of alpha-lactalbumin by solution X-ray scattering
24. 2+-dependent compaction and folding of yeast tRNA(Phe) and the catalytic domain of the B. subtilis RNase P RNA determined by small-angle X-ray scattering
25. Small angle X-ray scattering reveals a compact intermediate in RNA folding
26. A partially unfolded structure of the alkaline-denatured state of pepsin and its implication for stability of the zymogen-derived protein
27. Role of hydrophobic interactions in yeast phosphoglycerate kinase stability
28. Compactness of protein molten globules: Temperature-induced structural changes of the apomyoglobin folding intermediate
29. Mutations can cause large changes in the conformation of a denatured protein
30. Anion-induced folding of Staphylococcal nuclease: Characterization of multiple equilibrium partially folded intermediates
31. The compact and expanded denatured conformations of apomyoglobin in the methanol-water solvent
32. Stability against denaturation mechanisms in halophilic malate dehydrogenase "adapt" to solvent conditions
33. Exploring the temperature-pressure phase diagram of staphylococcal nuclease
34. Compactness of the denatured state of a fast-folding protein measured by submillisecond small-angle X-ray scattering
35. Chain collapse can occur concomitantly with the rate-limiting step in protein folding
36. The radius of gyration of an apomyoglobin folding intermediate
37. Kinetic refolding of beta-lactoglobulin, studies by synchrotron X-ray scattering, and circular dichroism, absorption and fluorescence spectroscopy
38. Kinetics of lysozyme refolding: Structural characterization of a non-specifically collapsed state using time-resolved X-ray scattering
39. Transient dimer in the refolding kinetics of cytochrome c characterized by small-angle X-ray scattering
40. Characterization of transient intermediates in lysozyme folding with time-resolved small-angle X-ray scattering
41. Trifluoroethanol-induced conformational transition of hen egg-white lysozyme studied by small-angle X-ray scattering
42. A lysozyme folding intermediate revealed by solution X-ray scattering
43. Chain-like conformation of heat-denatured ribonuclease A and cytochrome c as evidenced by solution X-ray scattering
44. Protein denaturation: A small-angle X-ray scattering study of the ensemble of unfolded states of cytochrome c
45. Multistep denaturation of Borrelia burgdorferi OspA, a protein containing a single-layer beta-sheet
46. pH-Dependent unfolding of aspergillopepsin II studied by small-angle X-ray scattering
47. Penetration function and second virial coefficient for linear and regular star polymers
48. Second virial coefficient: Variations with lysozyme crystallisation conditions
49. CRYSOL - A program to evaluate X-ray solution scattering of biological macromolecules from atomic coordinates
50. Protein hydration in solution: Experimental observation by X-ray and neutron scattering
51. Study of thermally and chemically unfolded conformations of a small β-protein by means of small-angle neutron scattering
52. 2O
53. Partial volumes and compressibilities of extended polypeptide chains in aqueous solution: Additivity scheme and implication of protein unfolding at normal and high pressure
54. How do small single-domain proteins fold?
55. 2+ pump
56. Part III: The behavior of biological macromolecules
57. NMR characterization of residual structure in the denatured state of protein L
58. Folding of a dimeric beta-barrel: Residual structure in the urea denatured state of the human papillomavirus E2 DNA binding domain
59. Towards a complete description of the structural and dynamic properties of the denatured state of barnase and the role of residual structure in folding
60. Structure and dynamics of an acid-denatured protein G mutant
61. Dimensions of protein random coils
62. Influence of the solvent on the conformational-dependent properties of random-coil polypeptides. I. The mean-square of the end-to-end distance and of the dipole moment
63. Synthetic polymers in solution
64. On volume changes accompanying conformational transitions in biopolymers
65. Volumetric and spectroscopic characterizations of the native and acid-induced denatured states of staphylcoccal nuclease
66. The energy landscape theory of protein folding: Insights into folding mechanisms and scenarios
67. Denaturation versus unfolding: Energetic aspects of residual structure in denatured alpha-lactalbumin
68. Energetics of the alpha-lactalbumin states: A calorimetric and statistical thermodynamic study
69. Small-angle X-ray scattering and calorimetric studies of thermal conformational change of lysozyme depending on pH
70. Reinvestigation of the proteolytic activity of neocarzinostatin
71. Improved signal-to-background ratio in small-angle X-ray scattering experiments with synchrotron radiation using an evacuated cell for solutions
72. How random is a highly denatured protein?
73. Small-angle-scattering-data treatment by the regularization method
74. Röntgenuntersuchung Gelöster Fadenmoleküle
75. Light scattering from wormlike chains with excluded volume effects
76. Form factor of an infinite Kratky-Porod chain
77. Scattering function of polystyrene
78. Scattering functions of semiflexible polymers with and without excluded volume effects
79. Critical exponents for the n-vector model in three dimensions from field theory
80. Stopped-flow solution scattering using synchrotron radiation: Apparatus, data collection and data analysis
81. Analysis of components present in kinetics (or titration) curves
82. X-ray scattering titration of the structure transition of aspartate transcarbamylase with a bisubstrate analogue. Influence of nucleotide effectors
83. Solution structure of the ternary complex between aminoacyl-tRNA, elongation factor Tu, and guanosine triphosphate