Rapid caspase-3 activation during apoptosis revealed using fluorescence-resonance energy transfer

EMBO Reports

Volumn 1, Issue 3, 2000, Pages 266-270

  Tyas, Lorraine ^a   Brophy, Victoria A ^a   Pope, Andrew ^b   Rivett, A Jennifer ^a   Tavaré, Jeremy M ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE; CASPASE 3; HYBRID PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; PEPTIDE FRAGMENT; STAUROSPORINE;

ANIMAL; APOPTOSIS; ARTICLE; CELL MEMBRANE POTENTIAL; CELL STRAIN COS1; ENZYME ACTIVATION; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; GENETICS; KINETICS; METABOLISM; MITOCHONDRION; SPECTROFLUOROMETRY;

ANIMALS; APOPTOSIS; CASPASE 3; CASPASES; COS CELLS; ENZYME ACTIVATION; KINETICS; MEMBRANE POTENTIALS; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; PEPTIDE FRAGMENTS; POLY(ADP-RIBOSE) POLYMERASES; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE; STAUROSPORINE; TRANSFECTION;

EID: 0034280126     PISSN: 1469221X     EISSN: None     Source Type: Journal    
DOI: 10.1093/embo-reports/kvd050     Document Type: Article

References (9)

1. Budihardjo, I., Oliver, H., Lutter, M., Luo, X. and Wang, X. (1999) Biochemical pathways of caspase activation during apoptosis. Annu. Rev. Cell. Dev. Biol., 15, 269-290.
2. Evan, G.I., Lewis, G.K., Ramsay, G. and Bishop, J.M. (1985) Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol., 5, 3610-3616.
3. Goldstein, J.C., Waterhouse, N.J., Juin, P., Evan, G.I. and Green, D.R. (2000) The coordinate release of cytochrome c during apoptosis is rapid, complete and kinetically invariant. Nature Cell Biol., 2, 156-162.
4. Green, D.R. and Reed, J.C. (1998) Mitochondria and apoptosis. Science, 281, 1309-1312.
5. Gurtu, V., Kain, S.R. and Zhang, G. (1997) Fluorimetric and colorimetric detection of caspase activity associated with apoptosis. Anal. Biochem., 251, 98-102.
6. Tsien, R.Y. (1998) The green fluorescent protein. Annu. Rev. Biochem., 67, 509-544.
7. Wolf, B.B. and Green, D.R. (1999) Suicidal tendencies: apoptotic cell death by caspase family proteinases. J. Biol. Chem., 274, 20049-20052.
8. Xu, X., Gerard, A.L., Huang, B.C., Anderson, D.C., Payan, D.G. and Luo, Y. (1998) Detection of programmed cell death using fluorescence energy transfer. Nucleic Acids Res., 26, 2034-2035.
9. Zou, H., Li, Y., Liu, X. and Wang, X. (1999) An APAF-1-cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9. J. Biol. Chem., 274, 11549-11556.