Fish cell lines as versatile tools in ecotoxicology: Assessment of cytotoxicity, cytochrome P4501A induction potential and estrogenic activity of chemicals and environmental samples

Toxicology in Vitro

Volumn 15, Issue 4-5, 2001, Pages 477-488

  Fent, K ^a,b  

^a SWISS FEDERAL INSTITUTE OF TECHNOLOGY   (Switzerland)

^b Wacht 24   (Switzerland)

Author keywords

Cytochrome P450; Cytotoxicity; Ecotoxicology; Endocrine disrupters; Estrogen receptor; Fish cell lines; Reporter gene assay; Xenoestrogens

Indexed keywords

1 AZAPYRENE; 1,6 DINITROPYRENE; 10 AZABENZO[A]PYRENE; 2 AZAFLUORANTHENE; 2 NITROFLUORENE; 2 NITRONAPHTHALENE; 3 NITROFLUORANTHENE; 6 NITROCHRYSENE; 7 AZAFLUORANTHENE; 7 NITROBENZO[A]ANTHRACENE; AZAARENE DERIVATIVE; BENZ[A]ACRIDINE; BENZ[A]ANTHRACENE; BENZ[C]ACRIDINE; BENZO[A]PYRENE; BENZO[H]QUINOLINE; CHRYSENE; CYTOCHROME P450; CYTOCHROME P450 1A; DIBENZ[A,C]ACRIDINE; DIBENZ[A,C]ANTHRACENE; DIBENZ[A,H]ACRIDINE; DIBENZ[A,H]ANTHRACENE; DIBENZ[A,J]ACRIDINE; ESTROGEN RECEPTOR BETA; FLUORANTHENE; POLYCYCLIC AROMATIC HYDROCARBON; POLYCYCLIC AROMATIC HYDROCARBON DERIVATIVE; PYRENE; UNCLASSIFIED DRUG; UNINDEXED DRUG;

ACCURACY; ANALYTIC METHOD; ANIMAL CELL; CANCER CELL CULTURE; CELL LEVEL; CONFERENCE PAPER; CONTROLLED STUDY; COST CONTROL; CYTOTOXICITY; ECOTOXICOLOGY; ENZYME INDUCTION; ESTROGEN ACTIVITY; FISH; GENETIC TRANSFECTION; GONAD; LIPOPHILICITY; LIVER CELL CARCINOMA; NONHUMAN; PROTEIN EXPRESSION; RAINBOW TROUT; REPORTER GENE; RISK;

ANIMAL TESTING ALTERNATIVES; ANIMALS; CELL LINE; CELL SURVIVAL; CYTOCHROME P-450 CYP1A1; DOSE-RESPONSE RELATIONSHIP, DRUG; ECOSYSTEM; ENZYME INDUCTION; ESTROGENS, NON-STEROIDAL; FISHES; GENES, REPORTER; HAZARDOUS SUBSTANCES; TOXICITY TESTS;

EID: 0034832754     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0887-2333(01)00053-4     Document Type: Conference Paper

References (63)

1. The adaptation of the permanent fish cell lines PLHC-1 and RTG-2 to FCS-free media results in similar growth rates compared to FCS-containing conditions
2. Untersuchungen über die verwendung von fischzellkulturen für toxizitätsbestimmungen zur einschränkung und ersatz von fischtests. Zentralblatt für bakteriologie und hygiene, I
3. Overview of a workshop on screening methods for detecting potential (anti-) estrogenic/androgenic chemicals in wildlife
4. In vitro cytotoxicity or organic pollutants to bluegill sunfish (BF-2) cells
5. In vitro cytotoxicity studies with the fish hepatoma cell line, PLHC-1 (Poeciliopsis lucida)
6. Technology and uses of cell cultures from the tissues and organs of bony fish
7. Toxicity determined in vitro by morphological alterations and neutral red absorption
8. EROD induction in cultured chick embryo liver: A sensitive bioassay for dioxin-like environmental pollutants
9. Cytotoxicity in vitro of organotin compounds to fish hepatoma cells PLHC-1 (Poeciliopsis lucida)
10. An ELISA for the determination of cytochrome P4501A in fish cell cultures
11. Induction of cytochrome P450 as a biomarker for environmental contamination in aquatic ecosystems
12. Correlations between the RTG-2 cytotoxicity test and in vivo LC50 rainbow trout bioassay
13. A sensitive zonagenic assay for trapid in vitro assessment of estrogenic potency of xenobiotics and mycotoxins
14. MEIC evaluation of acute systemic toxicity. Part III. In vitro results from 16 additional methods used to test the first 30 reference chemicals and a comparative cytotoxicity analysis
15. Comparison of toxic equivalent factors for selected dioxin and furan congeners deriving using fish and mammalian liver cell lines
16. Correlation of the neutral red uptake inhibition assay of cultured fathead minnow fish cells with fish lethality data
17. Estrogenic activity of chlordecone, o,p′-DDT and o,p′-DDE in juvenile rainbow trout: Induction of vitellogenesis and interaction with hepatic estrogen binding sites
18. Ecotoxicology of organotin compounds
19. Cytochrome P4501A induction potencies of polycyclic aromatic hydrocarbons in a fish hepatoma cell line: Demonstration of additive interactions
20. Cytotoxicity of organic environmental chemicals to fish liver cells (PLHC-1)
21. Long-term effects of nonylphenol on vitellogenin and zona radiata protein expression in juvenile rainbow trout
22. Monolayer and aggregate cultures of rainbow trout hepatocytes: Long-term and stable liver-specific expression in aggregates
23. Influence of xenobiotics on rainbow trout liver estrogen receptor and vitellogenin gene expression
24. Exoestrogens: Mechanisms of action and strategies for identification and assessment
25. Disruption of sexual differentiation in genetic male common carp (Cyprinus carpio) exposed to an alkylphenol during different life stages
26. Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication
27. Organization versus activation: The role of endocrine-disrupting contaminants (EDCs) during embryonic development in wildlife
28. The aryl hydrocarbon receptor: A comparative perspective
29. Cytochrome P4501A induction and inhibition by 3,3′,4,4′-tetrachlorobiphenyl in an Ah receptor-containing fish hepatoma cell line (PLHC-1)
30. Rapid assessment of induced cytochrome P4501A protein and catalytic acitvity in fish hepatoma cells grown in multiwell plates: Response to TCDD, TCDF and two planar PCBs
31. Estrogenic activity in five United Kingdom rivers detected by measurement of vitellogenesis in caged male trout
32. Serum alters the uptake and relative potencies of halogenated aromatic hycrocarbons in cell culture bioassays
33. Recent applications of fish cell culture to biomedical research
34. Cytochrome P4501A induction and porphyrin accumulation in PLHC-1 fish cells exposed to sediment and oil shale extracts
35. Detergent components in sewage effluent are weakly oestrogenic to fish: An in vitro study, using rainbow trout (Oncorhynchus mykiss) hepatocytes
36. Inhibition of testicular growth in rainbow trout (Oncorhynchus mykiss) exposed to estrogenic alkylphenolic chemicals
37. Widespread sexual disruption in wild fish
38. Cytochrome P450 induction by nitrated polycyclic aromatic hydrocarbons, azaarenes, and binary mixtures in fish heptatoma cell line PLHC-1
39. Kultivierung einer permanenten fischzellinie in serum-freien medium: Spezielle erfahrungen mit einem zytotoxizitätstest für abwasserproben
40. Rainbow trout estrogen receptor presents an equal specificity but a differential sensitivity for estrogens than human estrogen receptor
41. Wood-derived estrogens: Studies in vitro with breast cancer cell lines and in vivo in trout
42. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
43. Environmental estrogenic effects of alkylphenol ethoxylates
44. Identification and estrogen induction of two estrogen receptors (ER) messenger ribonucleic acids in the rainbow trout liver: Sequence homology with other ERs
45. Two complementary bioassays for screening the estrogenic potency of xenobiotics: Recombinant yeast for trout estrogen receptor and trout hepatocyte cultures
46. Estrogenic effects of effluents from sewage treatment works
47. Evaluation of heavy-metal ion toxicity in fish cells using a combined stress protein and cytotoxicity assay
48. Polychlorinated biphenyls (PCBs): Environmental impact, biochemical and toxic responses, and implications for risk assessment
49. In vitro cytotoxicity of chlorophenols to goldfish GF-scale (GFS) cells and quantitative structure-activity relationships
50. 1 cell line
51. The human estrogen receptor can regulate exogenous but not endogenous vitellogenin gene promoters in a Xenopus cell line
52. In vitro vitellogenin production by carp (Cyprinus carpio) hepatocytes as a screening method for determining (anti)estrogenic activity of xenobiotics
53. Estrogenic potencies of several environmental pollutants, as determined by vitellogenin induction in a carp hepatocyte assay
54. Feminized responses in fish to environmental estrogens
55. Vitellogenesis as a biomarker for estrogenic contamination of the aquatic environment
56. Endocrine disruption in wildlife: A critical review of the evidence
57. Aryl hydrocarbon receptor-mediated toxic potency of dissolved lipophilic organic contaminants collected from Lincoln creek, Milwaukee, Wisconsin, USA, to PLHC-1 (Poeciliopsis lucida) fish hepatoma cells
58. Established eurythermic line of fish cells in vitro
59. Relative potencies of individual polychlorinated dibenzo-p-dioxin, dibenzofuran, and biphenyl congeners and congener mixtures based on induction of cytochrome P4501A mRNA in a rainbow trout gonadal cell line (RTG-2)
60. In vitro bioassays for assessing estrogenic substances