An ELISA assay for cytochrome P4501A in fish liver cells

Environmental Toxicology and Chemistry

Volumn 15, Issue 4, 1996, Pages 592-596

  Brüschweiler, Beat J ^a   Würgler, Friedrich E ^b   Fent, Karl ^a  

^a ETH ZURICH   (Switzerland)

^b UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

CYP1A; ELISA; EROD; Fish hepatoma cells; PCBs

Indexed keywords

3 METHYLCHOLANTHRENE; CYTOCHROME P450; FLAVONE DERIVATIVE; POLYCHLORINATED BIPHENYL;

ANIMAL CELL; CONFERENCE PAPER; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; ECOTOXICITY; ENVIRONMENTAL IMPACT ASSESSMENT; ENZYME LINKED IMMUNOSORBENT ASSAY; LIVER CELL; NONHUMAN; PRIORITY JOURNAL; SCREENING TEST; SEA POLLUTION;

ANIMALIA;

EID: 0029874347     PISSN: 07307268     EISSN: None     Source Type: Journal    
DOI: 10.1897/1551-5028(1996)015<0592:AEAFCP>2.3.CO;2     Document Type: Conference Paper

References (28)

1. Bradlaw, J.A., L.H. Garthoff, N.E. Hurley and D. Firestone. 1980. Comparative induction of aryl hydrocarbon hydroxylase activity in vitro by analogues of dibenzo-p-dioxin. Food Cosmet. Toxlcol. 18:627-635.
2. Sawyer, T. and S. Safe. 1982. PCB isomers and congeners: Induction of aryl hydrocarbon hydroxylase and ethoxyresorufin-O-deethylase enzyme activities in rat hepatoma cells. Toxicol. Lett. 13:87-94.
3. Bandiera, S., T. Sawyer, M. Romkes, B. Zmudzka, L. Safe, G. Mason, B. Keys and S. Safe. 1984. Polychlorinated dibenzofurans (PCDFs): Effects of structure on binding to the 2,3,7,8-TCDD cytosolic receptor protein, AHH induction and toxicity. Toxicology 32:131-144.
4. Safe, S. 1990. Polychlorinated biphenyls (PCBs). dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and related compounds: Environmental and mechanistic considerations which support the development of toxic equivalency factors (TEFs). CRC Crit. Rev. Toxicol. 21:51-88.
5. Tillitt, D.E., J.P. Giesy and G.T. Ankley. 1991. Characterization of the H4IIE rat hepatoma cell bioassay as a tool for assessing toxic potency of planar halogenated hydrocarbons in environmental samples. Environ. Sci. Technol. 25:87-92.
6. Tillitt, D.E., G.T. Ankley, D.A. Verbrugge, J.P. Giesy, J.P. Ludwig and T.J. Kubiak. 1991. H4IIE rat hepatoma cell bioassay-derived 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents in colonial fish- eating waterbird eggs from the Great Lakes. Arch. Environ. Contam. Toxicol. 21:91-101.
7. Gooch, J.W., A.A. Elskus, P.J. Kloepper-Sams, M.E. Hahn and J.J. Stegeman. 1989. Effects of ortho- and non-ortho-substituted polychlorinated biphenyl congeners on the hepatic monooxygenase system in scup (Stenotomus chrysops). Toxicol. Appl. Pharmacol. 98:422-433.
8. Walker, M.K. and R.E. Peterson. 1991. Potencies of poly chlorinated dibenzo-p-dioxin, dibenzofuran, and biphenyl congeners, relative to 2,3,7,8-tetrachlorodibenzo-p-dioxin, for producing early life stage mortality in rainbow trout (Oncorhynchus mykiss). Aquat. Toxicol. 21:219-238.
9. Pesonen, M., A. Goksøyr and T. Andersson. 1992. Expression of P4501A1 in a primary culture of rainbow trout hepatocytes exposed to β-naphthoflavone or 2,3,7,8-tetrachlorodibenzo-p-dioxin. Arch. Biochem. Biophys. 292:228-233.
10. Miranda, C.L., P. Collodi, X. Zhao, D.W. Barnes and D.R. Buhler. 1993. Regulation of cytochrome P450 expression in a novel liver cell line from zebrafish (Brachydanio rerio). Arch. Biochem. Biophys. 305:320-327.
11. Lee, L.E J., J.H. demons, D.G. Bechtel, S.J. Caldwell, K. Han, M. Pasitschniak-Arts, D.D. Mosser and N.C. Bols. 1993. Development and characterization of a rainbow trout liver cell line expressing cytochrome P450- dependent monooxygenase activity. Cell Biol. Toxicol. 9:279-294
12. Ryan, J.A. and L.E. Hightower. 1994. Evaluation of heavy metal ion toxicity in fish cells using a combined stress and cytotoxicity assay. Environ. Toxicol. Chem. 13:1231-1240.
13. Babich, H., D.W. Rosenberg and E. Borenfreund. 1991. In vitro cytotoxicity studies with the fish hepatoma cell line PLHC-1 (Poeciliopsis lucida). Ecotoxicol. Environ. Saf. 21:327-336.
14. Hahn, M.E., T.M. Lamb, M.E. Schultz, R.M. Smolowitz and J.J. Stegeman. 1993. Cytochrome P4501A induction and inhibition by 3,3′,4,4′-tetrachlorobiphenyl in an Ah receptor-containing fish hepatoma cell line (PLHC-1). Aquat. Toxicol. 26:185-208.
15. Tillitt, D.E. and S.M. Cantrell. 1992. Planar halogenated hydrocarbon (PHH) structure activity relationship in a teleost (PLHC-1) cell line. Abstracts, 13th Annual Meeting, Society of Environmental Toxicology and Chemistry, Cincinnati, OH, USA, November 8-12, p. 45.
16. Kennedy, S.W., A. Lorenzen, C.A. James and B.T. Collins. 1993. Ethoxyresorufin-O-deethylase and porphyrin analysis in chicken embryo hepatocyte cultures with a fluorescence multiwell plate reader. Anal. Biochem. 211:102-112.
17. Hahn, M.E. 1994. Cytochrome P450 induction and inhibition by planar halogenated aromatic hydrocarbons in a fish cell line: Promise and pitfalls for environmental testing. In Vitro Cell. & Dev. Biol. 30A:39-40.
18. Celander, M. and L. Förlin. 1991. Catalytic activity and immunochemical quantification of hepatic cytochrome P-450 in β-naphthoflavone and isosafrol treated rainbow trout (Oncorhynchus mykiss). Fish Physiol. Biochem. 9:189-197.
19. Br̈schweiler, B.J., F.E. Würgler and K. Fent. 1995. Cytotoxicity in vitro of organotin compounds to fish hepatoma cells PLHC-1 (Poeciliopsis lucida). Aquat. Toxicol. 32:143-160.
20. Saad, B., F.A. Scholl, H. Thomas, H. Schawalder, V. Streit, F. Waechter and P. Maier. 1993. Crude liver membrane fractions and extracellular matrix components as substrata regulate differentially the preservation and inducibility of cytochrome P-450 isoenzymes in cultured rat hepatocytes. Eur. J. Biochem. 213:805-814.
21. Shopsis, C. and E. Eng. 1985. Rapid cytotoxicity testing using a semi-automated protein determination on cultured cells. Toxicol. Lett. 26:1-8.
22. Kemeny, D.M. 1991. A Practical Guide to ELISA. Pergamon, Oxford, UK.
23. Herrero, M.E. and J.V. Castell. 1994. Quantification of CYP1A1 and 2B1/2 in rat hepatocytes cultured in microwells by immunological methods. Toxicol. In Vitro 8:1167-1175.
24. Hahn, M.E., B.L. Woodward, J.J. Stegeman and S.W. Kennedy. 1996. Rapid assessment of induced cytochrome P4501A protein and catalytic activity in fish hepatoma cells grown in multi-well plates. Environ. Toxicol. Chem. 15:582-591.
25. Rodman, L.E., S.I. Shedlofsky, A.T. Swim and L.W. Robertson. 1989. Effects of polychlorinated biphenyls on cytochrome P450 induction in the chick embryo hepatocyte culture. Arch. Biochem. Biophys. 275:252-262.
26. Sinclair, P., J. Frezza, J. Sinclair, W. Bement, S. Haugen, J. Healey and H. Bonkovsky. 1989. Immunochemical detection of different isoenzymes of cytochrome P-450 induced in chick hepatocyte cultures. Biochem. J. 258:237-245.
27. Voorman, R. and S.D. Aust. 1987. Specific binding of polyhalogenated aromatic hydrocarbon inducers of cytochrome P-450d to the cytochrome and inhibition of its estradiol 2-hydroxylase activity. Toxicol. Appl. Pharmacol. 90:69-78.
28. Bucheli, T.D. and K. Fent. 1995. Induction of cytochrome P450 as a biomarker for environmental contamination in aquatic ecosystems. Crit. Rev. Environ. Sci. Technol. 25:201-268.