Glucoamylase: Structure/function relationships, and protein engineering

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1543, Issue 2, 2000, Pages 275-293

  Sauer, Jørgen ^a   Sigurskjold, Bent W ^b   Christensen, Ulla ^c   Frandsen, Torben P ^d   Mirgorodskaya, Ekaterina ^e   Harrison, Matt ^e   Roepstorff, Peter ^e   Svensson, Birte ^a  

^a CARLSBERG LABORATORY   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

^d NOVO NORDISK A S   (Denmark)

^e UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

Bifunctional inhibitor; Binding loop; Catalytic base; Isothermal titration calorimetry; Mass spectrometry; Molecular recognition; O Glycosylated linker; Pre steady state kinetics; Sequence replacement variant; Site directed mutagenesis; Three dimensional structure

Indexed keywords

BETA CYCLODEXTRIN; FRUCTOSE; GLUCAN 1,4 ALPHA GLUCOSIDASE; GLUCOSE; HYDROLASE; PROTEIN; STARCH;

ASPERGILLUS AWAMORI; ASPERGILLUS NIGER; BINDING SITE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STRUCTURE; GENETIC ENGINEERING; KINETICS; NONHUMAN; PRIORITY JOURNAL; REVIEW;

AMINO ACID SEQUENCE; ASPERGILLUS; BINDING SITES; CARBOHYDRATE SEQUENCE; ENZYME INHIBITORS; GLUCAN 1,4-ALPHA-GLUCOSIDASE; HYDROGEN-ION CONCENTRATION; ISOENZYMES; MALTOSE; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MUTATION; OLIGOSACCHARIDES; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN ENGINEERING;

EID: 0034731427     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(00)00232-6     Document Type: Review

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