Mutational modulation of substrate bond-type specificity and thermostability of glucoamylase from Aspergillus awamori by replacement with short homologue active site sequences and thiol/disulfide engineering

Biochemistry

Volumn 35, Issue 26, 1996, Pages 8696-8704

  Fierobe, Henri Pierre ^a   Stoffer, Bjarne B ^b   Frandsen, Torben P ^a   Svensson, Birte ^a  

^a CARLSBERG LABORATORY   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL PROTEIN; GLUCAN 1,4 ALPHA GLUCOSIDASE; OLIGOSACCHARIDE; THIOL DERIVATIVE;

ARTICLE; ASPERGILLUS AWAMORI; DISULFIDE BOND; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME SUBSTRATE; FUNGUS GROWTH; FUNGUS MUTANT; GENETIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; DNA PRIMERS; ENZYME STABILITY; GLUCAN 1,4-ALPHA-GLUCOSIDASE; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OLIGOSACCHARIDES; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 0029900198     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi960241c     Document Type: Article

References (51)

1. Aleshin, A., Golubev, A., Firsov, L. M., & Honzatko, R. B. (1992) J. Biol. Chem. 267, 19291-19298.
2. Aleshin, A. E., Firsov, L. M., & Honzatko, R. B. (1994) J. Biol. Chem. 269, 15631-15639.
3. Berland, C. R., Sigurskjold, B. W., Stoffer, B., Frandsen, T. P., & Svensson B. (1995) Biochemistry 34, 10153-10161.
4. Boel, E., Hjort, I., Svensson, B., Norris, F., Norris, K. E., & Fiil, N. P. (1984) EMBO J. 3, 1097-1102.
5. Clarke, A. J., & Svensson, B. (1984a) Carlsberg Res. Commun. 49, 111-122.
6. Clarke, A. J., & Svensson, B. (1984b) Carlsberg Res. Commun. 49, 559-566.
7. Clarke, J., Henrick, K., & Fersht, A. R. (1995) J. Mol. Biol. 253, 493-504.
8. Eder, J., & Wilmanns, M. (1992) Biochemistry 31, 4437-4444.
9. Fagerström, R. (1991) J. Gen. Microbiol. 137, 1001-1008.
10. Fagerström, R., & Kalkkinen, N. (1995) Biotechnol. Appl. Biochem. 21, 223-231.
11. Fox, J. D., & Robyt, J. F. (1991) Anal. Biochem. 195, 93-96.
12. Frandsen, T. P., Dupont, C., Lehmbeck, J., Stoffer, B., Sierks, M. R., Honzatko, R. B., & Svensson, B. (1994) Biochemistry 33, 13808-13816.
13. Frandsen, T. P., Christensen, T., Stoffer, B., Lehmbeck, J., Dupont, C., Honzatko, R. B., & Svensson, B. (1995) Biochemistry 34, 10162-10169.
14. Harris, E. M. S., Aleshin, A., Firsov, L. M., & Honzatko, R. B. (1993) Biochemistry 32, 1618-1626.
15. Henrissat, B., Coutinho, P. M., & Reilly, P. J. (1994) Protein Eng. 7, 1281-1282.
16. Higuchi, R., Krummel, B., & Saiki, R. K. (1988) Nucleic Acids Res. 16, 7351-7367.
17. Hiromi, K., (1970) Biochem. Biophys. Res. Commun. 40, 1-6.
18. Hiromi, K., Onishi, M., & Tanaka, X. (1983) Mol. Cell. Biochem. 51, 79-95.
19. Innis, M. A., Holland, M. J., McCabe, P. C., Cole, G. E., Wittman, V. P., Tal, R., Watt, W. K., Gelfand, D. H., Holland, J. P., & Meade, J. H. (1985) Science 228, 21-26.
20. Ito, H., Fukuda, Y., Murata, K., & Kimura, A. (1983) J. Bacteriol. 153, 163-168.
21. Itoh, T., Ohtsuki, I., Yamashita, I., & Fukui, S. (1987) J. Bacteriol. 169, 4171-4176.
22. Joutsjoki, V. V., & Torkkeli, T. K. (1992) FEMS Micmbiol. Lett. 99, 237-244.
23. Kanaya, S., Katsuda, C., Kimura, S., Nakai, T., Kitakuni, E., Nakamura, H., Katayanagi, K., Morikawa, K., & Ikehara, M., (1991) J. Biol. Chem. 266, 6038-6044.
24. Matsumura, M., Becktel, W. J., Levitt, M., & Matthews, B. W. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 6562-6566.
25. Meagher, M. M., Nikolov, Z. V., & Reilly P. J. (1989) Biotechnol. Bioeng. 34, 681-688.
26. Mitchinson, C., & Wells, J. A. (1989) Biochemistry 28, 4807-4815.
27. Munch, O., & Tritsch, D. (1990) Biochim. Biophys. Acta 1041, 111-116.
28. Nunberg, J. H., Meade, J. H., Cole, G., Lawyer, F. C., McCabe, P., Schweickart, V., Tal, R., Wittman, V. P., Flatgaard, J. E., & Innis, M. (1984) Mol. Cell. Biol. 4, 2306-2315.
29. Palcic, M. M., Skrydstrup, T., Bock, K., Le, N., & Lemieux, R. U. (1993) Carbohydr. Res. 250, 87-92.
30. Riddles, P. W., Blakeley, R. L., & Zerner, B. (1983) Methods Enzymol. 91, 49-60.
31. Sauer, R. T., Hehir, K., Stearman, R. S., Weiss, M. A., Jeitler-Nilsson, A., Suchanek., E. G., & Pabo, C. O. (1986) Biochemistry 25, 5992-5998.
32. Sierks, M. R., & Svensson, B. (1993) Biochemistry 32, 1113-1117.
33. Sierks, M. R., & Svensson, B. (1994) Protein Eng. 7, 1479-1484.
34. Sierks, M. R., Ford, C., Reilly, P. J., & Svensson, B. (1989) Protein Eng. 2, 621-625.
35. Sierks, M. R., Ford, C., Reilly, P. J., & Svensson, B. (1990) Protein Eng. 3, 193-198.
36. Sierks, M. R., Ford, C., Reilly, P. J., & Svensson, B. (1993) Protein Eng. 6, 75-79.
37. Stoffer, B. (1994) Ph.D. Thesis, University of Copenhagen, Faculty of Science, Denmark.
38. Stoffer, B., Frandsen, T. P., Busk, P. K., Schneider, P., Svendsen, I., & Svensson, B. (1993) Biochem. J. 292, 197-202.
39. Stoffer, B., Aleshin, A. E., Firsov, L. M., Svensson, B., & Honzatko, R. B. (1995) FEBS Lett. 358, 57-61.
40. Svensson, B., Larsen, K., Svendsen, I., & Boel, E. (1983) Carlsberg Res. Commun. 48, 529-544.
41. Svensson, B., Larsen, K., & Gunnarsson, A. (1986) Eur. J. Biochem. 154, 497-502.
42. Svensson, B., Jespersen, H., Sierks, M. R., & MacGregor, A. (1989) Biochem. J. 264, 309-311.
43. Svensson, B., Clarke, A. J., Svendsen, I., & Møller, H. (1990) Eur. J. Biochem. 188, 29-38.
44. Svensson B., Frandsen, T. P., Matsui, I., Juge, N., Fierobe, H.-P., Stoffer, B., & Rodenburg K. W. (1995) Carbohydrate Bioengi- neering (Pelersen, S. B., Svensson, B., & Pedersen, S., Eds.) pp 125-145, Elsevier Science B. V., Amsterdam, The Netherlands.
45. Søgaard, M., & Svensson, B. (1990) Gene 94, 173-179.
46. Villafranca, J. E., Howell, E. E., Voet, D. H., Strobel, M. S., Ogden, R. C., Abelson, J. N., & Kraut, J. (1983) Science 222, 782-788.
47. Villafranca, J. E., Howell, E. E., Oatley, S. J., Xuong, N., & Kraut, J. (1987) Biochemistry 26, 2182-2189.
48. Wakarchuk, W. W., Sung, W. L., Campbell, R. L., Cunningham, A., Watson, D. C., & Yaguchi, M. (1994) Protein Eng. 7, 1379-1386.
49. Weill, C. E., Burch, R. J., & van Dyk, J. W. (1954) Cereal Chem. 31, 150-158.
50. Williamson, G., Belshaw, N. J., & Williamson, M. P. (1992a) Biochem. J. 282, 423-428.
51. Williamson, G., Belshaw, N. J., Noel, T. R., Ring, S. G., & Williamson, M. P. (1992b) Eur. J. Biochem. 207, 661-670.