Ligand-induced stabilization of PPARγ monitored by NMR spectroscopy: Implications for nuclear receptor activation

Journal of Molecular Biology

Volumn 298, Issue 2, 2000, Pages 187-194

  Johnson, Bruce A ^a   Wilson, Eileen M ^a   Li, Ying ^a   Moller, David E ^a   Smith, Roy G ^a   Zhou, Gaochao ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

NMR spectroscopy; Nuclear receptor; PPAR ; Protein dynamics; Transcriptional activation

Indexed keywords

CELL NUCLEUS RECEPTOR; HORMONE; INSULIN; LIGAND; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR; ROSIGLITAZONE; TRANSCRIPTION FACTOR;

ARTICLE; BINDING SITE; HUMAN; LIGAND BINDING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTON NUCLEAR MAGNETIC RESONANCE; X RAY CRYSTALLOGRAPHY;

EID: 0034724560     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3636     Document Type: Article

References (36)

1. Hormone and antihormone induce distinct conformational changes which are central to steroid receptor activation
2. Dominant negative mutations in human PPARγ associated with severe insulin resistance, diabetes mellitus and hypertension
3. Thiazolidinediones produce a conformational change in peroxisomal proliferator-activated receptor-γ: Binding and activation correlate with antidiabetic actions in db/db mice
4. Retinoic acid receptor: A simulation analysis of retinoic acid binding and the resulting conformational changes
5. Molecular basis of agonism and antagonism in the oestrogen receptor
6. Both coactivator LXXLL motif-dependent and -independent interactions are required for PPARγ function
7. NMRPipe: A multidimensional spectral processing system based on UNIX pipes
8. Molecular cloning, expression and characterization of human peroxisome proliferator activated receptors γ1 and γ2
9. Statistical thermodynamic linkage between conformational and binding equilibria
10. Coactivator peptides hava differential stabilizing effect on the binding of estrogens and anti-estrogens with the estrogen receptor
11. 1H exchange
12. NMRView: A computer program for the visualization and analysis of NMR data
13. MOLMOL: A program for display and analysis of macromolecular structures
14. Mouse retinoid X receptor contains a separable ligand-binding and transactivation domain in its E region
15. The nuclear receptor superfamily: The second decade
16. Analysis of estrogen receptor function in vitro reveals three distinct classes of antiestrogens
17. Gradient-enhanced triple-resonance three-dimensional NMR experiments with improved sensitivity
18. Improved HSQC experiments for the observation of exchange broadened signals
19. Ligand binding and co-activator assembly of the peroxisome proliferator-activated receptor-γ
20. A peroxisome proliferator-activated receptor γ ligand inhibits adipocyte differentiation
21. NMR exchange broadening arising from specific low affinity protein self-association: Analysis of nitrogen-15 nuclear relaxation for rat CD2 domain 1
22. Solution structure of the KIX domain of CBP bound to the transactivation domain of CREB: A model for activator:coactivator interactions
23. 2H-decoupling
24. Interdomain communication regulating ligand-binding by PPARγ
25. Crystal structure of the ligand-binding domain of the human nuclear receptor PPARγ
26. A structural role for hormone in the thyroid hormone receptor
27. Interactions controlling the assembly of nuclear-receptor hetero-dimers and co-activators
28. The structure-activity relationship between peroxisome proliferator-activated receptor γ agonism and the antihyperglycemic activity of thiazolidinediones
29. 13C chemical-shift data
30. Intrinsically unstructured proteins: Re-assessing the protein structure-function paradigm
31. A canonical structure for the ligand-binding domain of nuclear receptors
32. Molecular recognition of fatty acids by peroxisom proliferator-activated receptors
33. 2H-labeled proteins with high sensitivity
34. 1H chemical shifts in proteins
35. Nuclear receptors have distinct affinities for coactivators: Characterization by fluorescense resonance energy transfer