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0342349850
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note
-
15N carrier at 117 ppm, 8 scans, 175 × 512 points, 1 h). Nearly complete aromatic and amide assignment was accomplished from 2D HSQC spectra for all complexes whereas only a partial assignment of the aliphatic region was achieved due to a larger signal overlap. All spectra were acquired on a Bruker DRX 800 spectrometer.
-
-
-
-
10
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-
0342349853
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-
note
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Upon binding of ascomycin, more than 90% of FKBP amide signals display nitrogen shifts larger than 0.15 ppm or proton shifts larger than 0.03 ppm.
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11
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0027403230
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Meadows, R. P.; Nettesheim, D.; Xu, X. R.; Olejniczak, E. T.; Petros, A. M.; Holzman, T. F.; Severin, J.; Gubbins, E.; Smith, H.; Fesik, S. W. Biochemistry 1993, 32, 754-765.
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12
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0030614915
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13
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0343655023
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note
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L protein.
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-
-
14
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0343655022
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note
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Using differential chemical shifts, it is possible to orient the ligands into the protein binding pocket when the series of ligands bind in a similar conformation and induce the same conformational changes in the protein. In those cases in which the ligands bind differently, chemical shift differences in a larger region of the protein would be expected, allowing these ligands to be quickly identified.
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15
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0032898682
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0004462977
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0033121310
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