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Volumn 288, Issue 5465, 2000, Pages 522-527

Transport of peptide-MHC class II complexes in developing dendritic cells

Author keywords

[No Author keywords available]

Indexed keywords

MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; PEPTIDE; T LYMPHOCYTE RECEPTOR;

EID: 0034697282     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.288.5465.522     Document Type: Article
Times cited : (425)

References (35)
  • 2
    • 0028313992 scopus 로고
    • P. Cresswell, Anna. Rev. Immunol. 12, 259 (1994); H. Chapman, Curr. Opin. Immunol. 10, 93 (1998).
    • (1994) Anna. Rev. Immunol. , vol.12 , pp. 259
    • Cresswell, P.1
  • 3
    • 0032005330 scopus 로고    scopus 로고
    • P. Cresswell, Anna. Rev. Immunol. 12, 259 (1994); H. Chapman, Curr. Opin. Immunol. 10, 93 (1998).
    • (1998) Curr. Opin. Immunol. , vol.10 , pp. 93
    • Chapman, H.1
  • 5
    • 0033523774 scopus 로고    scopus 로고
    • J. Stinchcombe and G. M. Griffiths, J. Cell Biol. 147, 1 (1999); R. Wubbolts et al., J. Cell Biol. 135, 611 (1996); G. Raposo et al., J. Exp. Med. 183, 1161 (1996).
    • (1999) J. Cell Biol. , vol.147 , pp. 1
    • Stinchcombe, J.1    Griffiths, G.M.2
  • 6
    • 0029837980 scopus 로고    scopus 로고
    • J. Stinchcombe and G. M. Griffiths, J. Cell Biol. 147, 1 (1999); R. Wubbolts et al., J. Cell Biol. 135, 611 (1996); G. Raposo et al., J. Exp. Med. 183, 1161 (1996).
    • (1996) J. Cell Biol. , vol.135 , pp. 611
    • Wubbolts, R.1
  • 7
    • 0029989258 scopus 로고    scopus 로고
    • J. Stinchcombe and G. M. Griffiths, J. Cell Biol. 147, 1 (1999); R. Wubbolts et al., J. Cell Biol. 135, 611 (1996); G. Raposo et al., J. Exp. Med. 183, 1161 (1996).
    • (1996) J. Exp. Med. , vol.183 , pp. 1161
    • Raposo, G.1
  • 9
    • 0030869567 scopus 로고    scopus 로고
    • P. Pierre et al., Nature 388, 787 (1997); M. Talmor et al., Eur. J. Immunol. 28, 811 (1998).
    • (1997) Nature , vol.388 , pp. 787
    • Pierre, P.1
  • 10
    • 0031881534 scopus 로고    scopus 로고
    • P. Pierre et al., Nature 388, 787 (1997); M. Talmor et al., Eur. J. Immunol. 28, 811 (1998).
    • (1998) Eur. J. Immunol. , vol.28 , pp. 811
    • Talmor, M.1
  • 12
    • 0032546352 scopus 로고    scopus 로고
    • J. Banchereau and R. M. Steinman, Nature 392, 245 (1998); I. Mellman, S. Turley, R. M. Steinman, Trends Cell Biol. 8, 231 (1998).
    • (1998) Nature , vol.392 , pp. 245
    • Banchereau, J.1    Steinman, R.M.2
  • 15
    • 0342576896 scopus 로고    scopus 로고
    • note
    • k+ DCs grown from C3H/HeN, C3H/HeJ, and CBA/J (Jackson Labs, Bar Harbor, ME) marrow suspensions were pulsed with HEL (1 to 3 mg/ml) with or without LPS (1 to 10 ng/ml, E. coli 0111.84) for 1 to 3 hours, washed, and chased in HEL-and LPS-free media. Similar results were obtained with DCs from each strain. DC cultures contained supernatant from mGM-CSF-expressing J558L cells (from A. Lanzavecchia, Basel Institute, Basel, Switzerland). LPS was removed from HEL (Sigma) with Kuttsuclean adsorbent (Manuha Corporation, Ibaraki, Japan). Maturation stimuli included LPS, CD40L, tumor necrosis factor-α, or replating (22).
  • 16
    • 0343011194 scopus 로고    scopus 로고
    • note
    • k (10-2.16) and fluorescein isothiocyanate (FITC)-conjugated mAb to CD86 (CL1). Isotype controls were purchased from Pharmingen. Biotinylated mAbs were detected with streptavidin-phycoerythrin. Fluorescence analysis was done with a FACSCalibur and CellQuest software.
  • 17
    • 0342576895 scopus 로고    scopus 로고
    • note
    • For ICM, DCs were adhered to Alcian blue-coated glass coverslips for 20 min at 37°C in serum-free media. Cell were fixed in 4% (wt/vol) PFA and permeabilized (for intracellular staining) in 0.05% saponin, 10 mM Hepes (pH 7.4), 10 mM glycine (pH 8.0), and 10% goat serum in RPMI 1640. Antibody incubations were done as described (6). Purified C4H3 was used for microscopy. To detect HEL, we used the mouse mAb 1B12, provided by P. Allen (Washington University, St. Louis, MO). Rabbit anti-H-2M (ULM) and rabbit anti-mouse I-A (RIV) have been described elsewhere (6). Hybridomas TIB126 (anti-MHC I), TIB99 (anti-Thy1.2), and TIB93 (anti-I-Ak) were obtained from American Type Culture Collection. Rabbit anti-CD18 was a gift from P. Blier (Boehringer Ingelheim, CT). Several mouse anti-rat mAbs were the gift of J. P. Soullilou (University of Nantes, Nantes, France), including OX-18 (anti-MHC I), antiThy1.1, and OX-6 (anti-MHC II). Rat B7 was detected with a CTLA-4-human IgG fusion protein (a gift of P. Linsley, Bristol Meyers Squibb) in combination with a FITC-conjugated horse anti-human IgG. Mouse B7-2 was detected with CL1. Rat lysosomes were stained with rabbit Igp120 antiserum (24). Irrelevant isotype-matched mAbs, preimmune rabbit serum, or normal human serum were used as negative controls in all experiments. Coverslips were mounted in Mowiol with DABCO (Calbiochem, La Jolla, CA), and fluorescence was analyzed with conventional and confocal (0.5-to 1.0-μm optical sections) Zeiss microscopes.
  • 18
    • 0343882706 scopus 로고    scopus 로고
    • note
    • DCs were treated with nocodazole (30 μM, Sigma) or Latrunculin B (0.4 to 10 μ/ml; Calbiochem, San Diego, CA) after the HEL pulse to allow internalization and immunogenic complex formation to occur.
  • 19
    • 0342576893 scopus 로고    scopus 로고
    • note
    • k.
  • 20
    • 0026756119 scopus 로고
    • C. V. Harding and H. J. Geuze, J. Cell Biol. 119, 531 (1992); M. J. Kleijmeer, V. M. Oorschot, H. J. Geuze, J. Invest. Dermatol. 103, 516 (1994); M. J. Kleijmeer, S. Morkowski, J. M. Griffith, A. Y. Rudensky, H. J. Geuze, J. Cell Biol. 139, 639 (1997).
    • (1992) J. Cell Biol. , vol.119 , pp. 531
    • Harding, C.V.1    Geuze, H.J.2
  • 21
    • 0028150614 scopus 로고
    • C. V. Harding and H. J. Geuze, J. Cell Biol. 119, 531 (1992); M. J. Kleijmeer, V. M. Oorschot, H. J. Geuze, J. Invest. Dermatol. 103, 516 (1994); M. J. Kleijmeer, S. Morkowski, J. M. Griffith, A. Y. Rudensky, H. J. Geuze, J. Cell Biol. 139, 639 (1997).
    • (1994) J. Invest. Dermatol. , vol.103 , pp. 516
    • Kleijmeer, M.J.1    Oorschot, V.M.2    Geuze, H.J.3
  • 26
    • 0032568806 scopus 로고    scopus 로고
    • P. Pierre and I. Mellman, Cell 93, 1135 (1998); M. Cella et al., Nature 388, 782 (1997); M. Rescigno et al., Proc. Natl. Acad. Sci. U.S.A. 95, 5229 (1998).
    • (1998) Cell , vol.93 , pp. 1135
    • Pierre, P.1    Mellman, I.2
  • 27
    • 0030858138 scopus 로고    scopus 로고
    • P. Pierre and I. Mellman, Cell 93, 1135 (1998); M. Cella et al., Nature 388, 782 (1997); M. Rescigno et al., Proc. Natl. Acad. Sci. U.S.A. 95, 5229 (1998).
    • (1997) Nature , vol.388 , pp. 782
    • Cella, M.1
  • 28
    • 0032574816 scopus 로고    scopus 로고
    • P. Pierre and I. Mellman, Cell 93, 1135 (1998); M. Cella et al., Nature 388, 782 (1997); M. Rescigno et al., Proc. Natl. Acad. Sci. U.S.A. 95, 5229 (1998).
    • (1998) Proc. Natl. Acad. Sci. U.S.A. , vol.95 , pp. 5229
    • Rescigno, M.1
  • 29
    • 0033538574 scopus 로고    scopus 로고
    • A. Grakoui et al., Science 285, 221 (1999).
    • (1999) Science , vol.285 , pp. 221
    • Grakoui, A.1
  • 31
  • 32
  • 35
    • 0343882702 scopus 로고    scopus 로고
    • note
    • We thank members of the Mellman, Steinman, and Inaba laboratories for valuable advice. Supported by grants from NIH (AI-34098 to I.M.), the National Institute of Allergy and Infectious Diseases (AI-13013 and AI-39672 to R.M.S.), and the Ministry of Education, Science and Culture of Japan (grants 08282104, 10153226, and 10044268 to K.I.). I.M. is a member of the Ludwig Institute for Cancer Research.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.