An open conformation of switch I revealed by the crystal structure of a Mg2+-free form of RHOA complexed with GDP: Implications for the GDP/GTP exchange mechanism

Journal of Biological Chemistry

Volumn 275, Issue 24, 2000, Pages 18311-18317

  Shimizu, Toshiyuki ^a   Ihara, Kentaro ^a   Maesaki, Ryoko ^a   Kuroda, Shinya ^a   Kaibuchi, Kozo ^a   Hakoshima, Toshio ^a  

^a NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; MAGNESIUM ION; RAS PROTEIN; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; DISSOCIATION; ENZYME STRUCTURE; PRIORITY JOURNAL;

BINDING SITES; CRYSTALLOGRAPHY, X-RAY; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; IMAGE PROCESSING, COMPUTER-ASSISTED; MAGNESIUM; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; RHOA GTP-BINDING PROTEIN; SPECTROMETRY, FLUORESCENCE;

EID: 0034674704     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M910274199     Document Type: Article

References (43)

1. Aelst, L. V., and D'Souza-Schorey, C. (1997) Genes Dev. 11, 2295-2322
2. Mackay, D. J. G., and Hall, A. (1998) J. Biol. Chem. 273, 20685-20688
3. Kaibuchi, K., Kuroda, S., and Amano, M. (1999) Annu. Rev. Biochem. 68, 459-486
4. Boriack-Sjodin, P. A., Margarit, S. M., Bar-Sagi, D., and Kuriyan, J. (1998) Nature 394, 337-343
5. Goldberg, J. (1998) Cell 95, 237-248
6. John, J., Rensland, H., Schlichting, I., Vetter, I., Borasio, G. D., Goody, R. S., and Wittinghofer, A. (1993) J. Biol. Chem. 268, 923-929
7. Simon, I., Zerial, M., and Goody R. S. (1996) J. Biol. Chem. 271, 20470-20478
8. Tucker, J., Sczakiel, G., Feuerstein, J., John, J., Goody, R. S., and Wittinghofer, A. (1986) EMBO J. 5, 1351-1358
9. Hall, A., and Self, A. J. (1986) J. Biol. Chem. 261, 10963-10965
10. John, J., Frech, M., and Wittinghofer, A. (1988) J. Biol. Chem. 263, 11792-11799
11. Pan, J. Y., Sanford, J. C., and Wessling-Resnick, M. (1996) J. Biol. Chem. 271, 1322-1328
12. Rudolph, M. G., Wittinghofer, A., and Vetter, I. R. (1999) Protein Sci. 8, 778-787
13. Wei, Y., Zhang, Y., Derewenda, U., Liu, X., Minor, W., Nakamoto, R. K., Somlyo, A. V., Somlyo, A. P., and Derewenda, Z. S. (1997) Nat. Struct. Biol. 4, 699-703
14. Ihara, K., Muraguchi, S., Kato, M., Shimizu, T., Shirakawa, M., Kuroda, S., Kaibuchi, K., and Hakoshima, T. (1998) J. Biol Chem. 273, 9656-9666
15. Matthews, B. W. (1968) J. Mol. Biol. 33, 491-497
16. Navaza, J. (1994) Acta Crystallogr. Sect. A 50, 157-163
17. Jones, T. A., Zou, J.-Y., Cowan, S. W., and Kjeldgaad, M. (1991) Acta Crystallogr. Sect. A 47, 110-119
18. Brunger, A. T., Adams, P. D., Clore, G. M., Delano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang J.-S., Kuszewski, J., Nilges, N., Pannu, N. S., Read, R. J., Rice, L. M., Simonson, T., and Warren, G. L. (1998) Acta Crystallogr. Sect. D Biol. Crystallogr. 54, 905-921
19. Laskowski, R. A., MacArthur, M. W., Moss, D. S., and Thornton, J. M. (1993) J. Appl. Crystallogr. 26, 283-291
20. Kraulis, P. J. (1991) J. Appl. Crystallogr. 24, 946-950
21. Merritt, E. A., and Murphy, M. E. P. (1994) Acta Crystallogr. Sect. D Biol. Crystallogr. 50, 869-873
22. Nicholls, A., Sharp, K. A., and Honig, B. (1991) Proteins Struct. Funct. Genet. 11, 281-296
23. Leonard, D. A., Evans, T., Hart, M., Cerione, R. A., and Manor, D. (1994) Biochemistry 33, 12323-12328
24. Rensland, H., Lautwein, A., Wittinghofer, A., and Goody, R. S. (1991) Biochemistry 30, 11181-11185
25. Hirshberg, M., Stockley, R. W., Dodson, G., and Webb, M. R. (1997) Nat. Struct. Biol. 4, 147-152
26. Cerione, R. A., and Zheng, Y. (1996) Curr. Opin. Cell Biol. 8, 216-222
27. Soisson, S. M., Nimnual, A. S., Uy, M., Bar-Sagi, D., and Kuriyan, J. (1998) Cell 95, 259-268
28. Liu, X., Wang, H., Eberstadt, M., Schunuchel, A., Olejniczak, E. T., Meadows, R. P., Schkeryantz, J. M., Janowick, D. A., Harian, J. E., Harris, E. A. S., Staunton, D. E., and Fesik, S. W. (1998) Cell 95, 269-277
29. Aghazadeh, B., Zhu, K., Kubiseski, T. J., Liu, G. A., Pawson, T., Zheng, Y., and Rosen, M. K. (1998) Nat. Struct. Biol. 5, 1098-1107
30. Sprang, S. R., and Coleman, D. E. (1998) Cell 95, 155-158
31. Pan, J. Y., and Wessling-Resnick, M. (1998) BioEssays 20, 516-521
32. Cherfils, J., and Chardin, P. (1999) Trends Biochem. Sci. 24, 306-311
33. Farnsworth, C. L., and Feig, L. A. (1991) Mol. Cell. Biol. 11, 4822-4829
34. Burstein, E. S., Brondyk, W. H., and Macara, I. G. (1992) J. Biol. Chem. 287, 22715-22718
35. Kornbluth, S., Dasso, M., and Newport, J. (1994) J. Cell Biol. 125, 705-719
36. Sprang, S. R. (1997) Annu. Rev. Biochem. 66, 639-678
37. Hoffman, G. R., Nassar, N., and Cerione, R. A. (2000) Cell 100, 345-356
38. Scheffzek, K., Stephan, I., Jensen, O. N., Illenberger, D., and Gierschik, P. (2000) Nat. Struct. Biol. 7, 122-126
39. Lambright, D. G., Noel, J. P., Hamm, H. E., and Sigler, P. B. (1994) Nature 369, 621-628
40. Mixon, M. B., Lee, E., Coleman, D. E., Berghuis, A. M., Gilman, A. G., and Sprang, S. R. (1995) Science 270, 954-959
41. Al-Karadaghi, S., Aevarsson, A., Garber, A., Zheltonosova, J., and Liljas, A. (1996) Structure 4, 555-565
42. Freymann, D. M., Keenan, R. J., Stroud, R. M., and Walter, P. (1999) Nat. Struct. Biol. 6, 793-801
43. Freymann, D. M., Keenan, R. J., Stroud, R. M., and Walter, P. (1997) Nature 385, 361-364