Compromise and accommodation in ecotin, a dimeric macromolecular inhibitor of serine proteases

Journal of Molecular Biology

Volumn 299, Issue 4, 2000, Pages 993-1003

  Gillmor, Sarah A ^a   Takeuchi, Toshihiko ^a   Yang, Steven Q ^a   Craik, Charles S ^a   Fletterick, Robert J ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Molecular recognition, specificity; Protease inhibitor complexes; Protein protein interactions; X ray structure

Indexed keywords

ALANINE; DIMER; ECOTIN; SERINE PROTEINASE; SERINE PROTEINASE INHIBITOR; TETRAMER; TRYPSIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; CONTROLLED STUDY; ENZYME INHIBITION; ENZYME INHIBITOR COMPLEX; ESCHERICHIA COLI; MOLECULAR RECOGNITION; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN PROTEIN INTERACTION; X RAY CRYSTALLOGRAPHY;

ESCHERICHIA COLI;

EID: 0034674167     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3812     Document Type: Article

References (27)

1. The crystal structure of the complex of blood coagulation factor VIIa with soluble tissue factor
2. Domain swapping: Entangling alliances between proteins
3. X-ray structures of a designed binding site in trypsin show metal-dependent geometry
4. Tracking conformational states in allosteric transitions of phosphorylase
5. Crystallographic R-factor refinement by molecular dynamics
6. The CCP4 suite: Programs for protein crystallography
7. Purification from Escherichia coli of a periplasmic protein that is a potent inhibitor of pancreatic proteases
8. Mechanism of inhibition of the human matrix metaltoproteinase stromelysin-1 by TIMP-1
9. Crystal structure of the thrombin-hirudin complex: A novel mode of serine protease inhibition
10. Hydrogen bonding and solvent structure in an antigen-antibody interface. Crystal structures and thermodynamic characterization of three Fv mutants complexed with lysozyme
11. The structure of protein-protein recognition sites
12. Dissecting the energetics of an antibody-antigen interface by alanine shaving and molecular grafting
13. Expression of the protease inhibitor ecotin and its co-crystallization with trypsin
14. Macromolecular chelation as an improved mechanism of protease inhibition: Structure of the ecotin-trypsin complex
15. MOLOC: A molecular modeling program
16. Protein folding and association: Insights from the interfacical and thermodynamic properties of hydrocarbons
17. Data collection and processing
18. Crystal structure of an ecotin-collagenase complex suggests a model for recognition and cleavage of the collagen triple helix
19. On the size of the active site in Proteases: I. Papain
20. Ecotin is a potent anticoagulant and reversible tight-binding inhibitor of factor Xa
21. Crystal structure analyses of uncomplexed ecotin in two crystal forms: Implications for its function and stability
22. The cystatins: Protein inhibitors of cysteine proteinases
23. Binding in the growth hormone receptor complex
24. Delocalizing trypsin specificity with metal activation
25. Engineered metal regulation of trypsin specificity
26. Engineering bidentate macromolecular inhibitors for trypsin and urokinase-type plasminogen activator
27. Ecotin: A serine protease inhibitor with two distinct and interacting binding sites