Delocalizing trypsin specificity with metal activation

Biochemistry

Volumn 35, Issue 19, 1996, Pages 5992-5998

  Willett, W Scott ^a,b   Brinen, Linda S ^a   Fletterick, Robert J ^a   Craik, Charles S ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF COLORADO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

APROTININ; CHYMOTRYPSIN; COPPER; HISTIDINE; METAL; NICKEL; PANCREAS ENZYME; PROTEINASE; TRYPSIN; TRYPSIN INHIBITOR; ZINC;

ANIMAL TISSUE; ARTICLE; BINDING SITE; CATALYSIS; COMPUTER MODEL; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; METAL BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; APROTININ; BACTERIAL PROTEINS; BASE SEQUENCE; COMPUTER SIMULATION; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI PROTEINS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OLIGODEOXYRIBONUCLEOTIDES; PERIPLASMIC PROTEINS; SERINE; SUBSTRATE SPECIFICITY; TRYPSIN; TYROSINE;

EID: 0029873173     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9530191     Document Type: Article

References (24)

1. Abrahmsen, L., Tom, J., Burnier, J., Butcher, K. A., Kossiakoff, A., & Wells, J. A. (1991) Biochemistry 30, 4151-4159.
2. Arnold, F. H. (1993) FASEB J. 7, 744-749.
3. Braxton, S., & Wells, J. A. (1992) Biochemistry 31, 7796-7801.
4. Brinen, L. S., Willett, W. S., Craik, C. S., & Fletterick, R. J. (1996) Biochemistry 35, 5999-6009.
5. Carter, P., & Wells, J. A. (1987) Science 237, 394-399.
6. Chakrabarti, P. (1990) Protein Eng. 4, 57-63.
7. Christianson, D. W., & Alexander, R. S. (1989) J. Am. Chem. Soc. 111, 6412-6419.
8. Corey, D. R., Willett, S., Coombs, G. S., & Craik, C. S. (1995) Biochemistry 34, 11521-11527.
9. Craik, C. S., Choo, Q. L., Swift, G. H., Qinto, C., MacDonald, R. J., & Rutter, W. J. (1984) J. Biol. Chem. 259, 14255-14264.
10. Craik, C. S., Largman, C., Fletcher, T., Roczniak, S., Barr, P. J., Fletterick, R., & Rutter, W. J. (1985) Science 228, 291-297.
11. Glusker, J. P. (1991) Adv. Protein Chem. 42, 1-76.
12. Hedstrom, L., Perona, J. J., & Rutter, W. J. (1994) Biochemistry 33, 8757-8763.
13. Higaki, J. N., Haymore, B. L., Chen, S., Fletterick, R. J., & Craik, C. S. (1990) Biochemistry 29, 8582-8586.
14. Kunkel, T. A. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 488-492.
15. Martell, A. D., & Smith, R. M. (1974) Critical Stability Constants, Plenum Press, New York.
16. McGrath, M. E., Hines, W. M., Sakanari, J. A., Fletterick, R. J., & Craik, C. S. (1991) J. Biol. Chem. 266, 6620-6625.
17. McGrath, M. E., Erpel, T., Bystroff, C., & Fletterick, R. J. (1994) EMBO J. 13, 1502-1507.
18. McPherson, A. (1989) Preparation and Analysis of Protein Crystals Robert E. Kreiger Publishing Co., Malabar, FL.
19. Paoni, N. F., Chow, A. M., Pena, L. C., Keyt, B. A., Zoller, M. J., & Bennett, W. F. (1993) Protein Eng. 6, 529-534.
20. Perona, J. J., & Craik, C. S. (1995) Protein Sci. 4, 337-360.
21. Perona, J. J., Hedstrom, L., Wagner, R. L., Rutter, W. J., Craik, C. S., & Fletterick, R. J. (1994) Biochemistry 33, 3252-3259.
22. Ponder, J. W., & Richards, F. M. (1987) J. Mol. Biol. 193, 775-791.
23. Wang, C. I., Yang, Q., & Craik, C. S. (1995) J. Biol. Chem. 270, 12250-12256.
24. Willett, W. S., Gillmor, S. A., Perona, J. J., Fletterick, R. J., & Craik, C. S. (1995) Biochemistry 34, 2172-2180.