Cytosine methylation of an Sp1 site contributes to organ-specific and cell-specific regulation of expression of the lung epithelial gene T1α

Biochemical Journal

Volumn 350, Issue 3, 2000, Pages 883-890

  Cao, Y X ^a   Jean, J C ^a   Williams, M C ^a  

^a BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

5 aza 2' deoxycytidine; Lung and brain gene; Lung cell lines; Promoter methylation

Indexed keywords

5 AZA 2' DEOXYCYTIDINE; BETA ACTIN; CELL MARKER; CYTOSINE; DNA; MESSENGER RNA; METHYLTRANSFERASE; TRANSCRIPTION FACTOR SP1;

ADOLESCENT; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN; CELL SPECIFICITY; CONTROLLED STUDY; DEMETHYLATION; DNA METHYLATION; DNA SEQUENCE; EMBRYO; FETUS; GENE EXPRESSION REGULATION; GENE SILENCING; LUNG ALVEOLUS CELL TYPE 1; LUNG ALVEOLUS CELL TYPE 2; NERVOUS SYSTEM DEVELOPMENT; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; RAT; REPORTER GENE; TRANSCRIPTION REGULATION;

ANIMAL; AZACITIDINE; BASE SEQUENCE; BLOTTING, NORTHERN; CELL LINE; CYTOSINE; DNA METHYLATION; DNA PRIMERS; EPITHELIAL CELLS; GENE EXPRESSION REGULATION; GENES, REPORTER; LUNG; MEMBRANE PROTEINS; PROMOTER REGIONS (GENETICS); RATS; RATS, SPRAGUE-DAWLEY; SUPPORT, U.S. GOV'T, P.H.S.; TRANSCRIPTION FACTOR, SP1;

EID: 0034664939     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3500883     Document Type: Article

References (47)

1. Cytosine methylation and mammalian development
2. The methyl-CpG binding protein MeCP2 is essential for embryonic development in the mouse
3. DNA methyltransferase in normal and Dnmt(n)/Dnmt(n) mouse embryos
4. Aberrant methylation of p16INK4a is an early event in lung cancer and a potential biomarker for early diagnosis
5. Tying it all together: Epigenetics, genetics, cell cycle, and cancer
6. Rett syndrome is caused by mutations in X-linked MECP2, encoding methylCpG-binding protein 2
7. A mammalian protein with specific demethylase activity for mCpG DNA
8. Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases
9. Epigenetic mechanisms underlying the imprinting of the mouse H19 gene
10. Genomic imprinting in mice: Its function and mechanism
11. Methylation of the minimal promoter of an embryonic globin gene silences transcription in primary erythroid cells
12. Tissue-specific transcriptional regulation of human leukosialin (CD43) gene is achieved by DNA methylation
13. Methyl-CpG-binding protein MeCP2 represses Sp1-activated transcription of the human leukosialin gene when the promoter is methylated
14. Transcriptional regulation of rat cyclin D1 gene by CpG methylation status in the promoter region
15. The activation of human gene MAGE-1 in tumor cells is correlated with genome-wide demethylation
16. MeCP2 is a transcriptional repressor with abundant binding sites in genomic chromatin
17. Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription
18. Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex
19. TGT3, thyroid transcription factor I, and Sp1 elements regulate transcriptional activity of the 1.3-kilobase pair promoter of T1a, a lung alveolar type I cell gene
20. Characterization of the gene and promoter for RTI40, a differentiation marker of type I alveolar epithelial cells
21. Keratinocyte growth factor increases mRNAs for SP-A and SP-B in adult rat type II cells in culture
22. Reversible transdifferentiation of alveolar epithelial cells
23. Loss of caveolin expression in type I pneumocytes as an indicator of subcellular alterations during ung fibrogenesis
24. Rat serum inhibits progression of alveolar epithelial cells toward the type I cell phenotype in vitro
25. Keratinocyte growth factor modulates alveolar epithelial cells in vitro: Expression of aquaporin 5 (AQP5)
26. Modulation of T1α expression with alveolar epithelial phenotype in vitro
27. 1.3 Kb of the lung type I cell T1α gene promoter mimics endogenous gene expression patterns during development but lacks sequences to enhance expression in perinatal and adult lung
28. The human DNA methyltransferases (DNMTs) 1, 3a and 3b: Coordinate mRNA expression in normal tissues and overexpression in tumors
29. SV40T-immortalized lung alveolar epithelial cells display post-transcriptional regulation of proliferation-related genes
30. Production of immortalized distal respiratory cell lines from surfactant C/simian virus 40 large tumor antigen transgenic mice
31. An improved method for isolating type II cells in high yield and purity
32. Monoclonal antibodies specific to apical surfaces of rat alveolar type I cells bind to surfaces of cultured, but not freshly isolated, type II cells
33. Cloning, characterization, and developmental expression of a rat lung alveolar type I cell gene in embryonic endodermal and neural derivatives
34. A genomic sequence protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands
35. Methylation-specific PCR: A novel PCR assay for methylation status in CpG islands
36. Cloning, characterization, and expression in Escherichia coli of the gene coding for the CpG DNA methylase from Spiroplasma sp. strain MQ1 (M.Sssl)
37. Cytosine methylation targeted to pre-determined sequences
38. Lack of expression from a retroviral vector after transduction of murine hemapoietic stem cell is associated with methylation in vivo
39. Demethylation and expression of methylated plasmid DNA stably transfected into HeLa cells
40. DNA methylation represses transcription in vivo
41. T1α protein is developmentally regulated and expressed by alveolar type I cells, choroid plexus, and ciliary epithelium of adult rats
42. The essentials of DNA methylation
43. CpG methylation, chromatin structure and gene silencing - A three-way connection
44. Increased cytosine DNA-methyltransferase activity is target-cell-specific and an early event in lung cancer
45. DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development
46. DNA methylation is a reversible biological signal