The methyl-CpG binding protein MeCP2 is essential for embryonic development in the mouse

Nature Genetics

Volumn 12, Issue 2, 1996, Pages 205-208

  Tate, Peri ^a   Skarnes, William ^a   Bird, Adrian ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOSOME PROTEIN; CYTOSINE; DNA BINDING PROTEIN; DNA METHYLTRANSFERASE; GUANOSINE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DNA METHYLATION; EMBRYO; EMBRYO DEVELOPMENT; GENE MUTATION; GENE TARGETING; MOUSE; NONHUMAN; PRIORITY JOURNAL; STEM CELL; X CHROMOSOME;

ANIMALS; BETA-GALACTOSIDASE; CHIMERA; CHROMOSOMAL PROTEINS, NON-HISTONE; DNA-BINDING PROTEINS; EMBRYONIC AND FETAL DEVELOPMENT; GENE TARGETING; LINKAGE (GENETICS); MALE; METHYL-CPG-BINDING PROTEIN 2; MICE; RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; RNA, MESSENGER; STEM CELLS; X CHROMOSOME;

EID: 0030071685     PISSN: 10614036     EISSN: None     Source Type: Journal    
DOI: 10.1038/ng0296-205     Document Type: Article

References (28)

1. Tate, P.H. & Bird, A.P. Effects of DNA methylation on DNA-binding proteins and gene expression. Curr. Opin. Genet. Dev. 3, 226-231 (1993).
2. Lewis, J.D. et al. Purification, sequence, and cellular localization of a novel chromosomal protein that binds to methylated DNA. Cell 69, 905-914 (1992).
3. Meehan, R.R., Lewis, J.D. & Bird, A.P. Characterization of MeCP2, a vertebrate DNA binding protein with affinity for methylated DNA. Nucl. Acids Res. 20, 5085-5092 (1992).
4. Nan, X., Meehan, R.R. & Bird, A. Dissection of the methyl-CpG binding domain from the chromosomal protein MeCP2. Nucl. Acids Res. 21, 4886-4892 (1993).
5. Nan, X., Tate, P., En Li & Bird, A. DNA methylation specifies chromosomal localization of MeCP2. Mol. Cell Biol. 16, 414-421 (1996).
6. Quaderi, N.A. et al. Genetic and physical mapping of a gene encoding a methyl CpG binding protein, Mecp2, to the mouse X chromosome. Genomics 22, 648-651 (1994).
7. Li. E., Sestor, T.H. & Jaenisch, R. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 69, 915-926 (1992).
8. Skarnes, W.C., Moss, J.E., Hurtley, S.M. & Beddington, R.S.P. Capturing genes in coding membrane and secreted proteins important for mouse development. Proc. Natl. Acad. Sci. USA 92, 6592-6596 (1995).
9. Mountford, P. et al. Dicistronic targeting constructs: reporters and modifiers of mammalian gene expression. Proc. Natl. Acad. Sci. USA 91, 4303-4307 (1994).
10. Schwartzberg, P.L., Robertson, E.J. & Goff, S.P. Targeted gene disruption of the endogenous c-abl locus by homologous recombination with DNA encoding a selectable fusion protein. Proc. Natl. Acad. Sci. USA 87, 3210-3214 (1990).
11. Jeannotte, L., Ruiz, J.C. & Robertson, E.J. Low level of Hox 1.3 gene expression does not preclude the use of promoterless vectors to generate a targeted gene disruption Mol. Cell Biol. 11, 5578-5585 (1991).
12. Robertson, E.J. Embryo-Derived Stem Cell Lines. In Teratocarcinomas and Embryonic Stem Cells, A Practical Approach. (ed. Robertson, E.J.) 71-112 (IRL Press, Oxford, 1987).
13. Rudnicki, M.A. & McBurney, M.W. Cell Culture Methods and Induction of Differentiation of Embryonal Carcinoma Cell Lines. In Teratocarcinomas and Embryonic Stem Cells, A Practical Approach. (ed. Robertson, E.J.) 19-49 (IRL Press, Oxford, 1987).
14. Miller, O.J., Schnedl, W., Allen, J. & Erlanger, B.F. 5-methylcytosine localised in mammalian constitutive heterochromatin. Nature 251, 636-637 (1974).
15. Stein, R., Razin, A. & Cedar, H. In vitro methylation of the hamster adenine phosphorybosy transferase gene inhibits its expression in mouse L cells. Proc. Natl. Acad. Sci. USA 79, 4418-3422 (1982).
16. Vardimon, L., Kressmann, A., Cedar, H., Maechler, M. & Doerfler, W. Expression of a cloned adenovirus gene is inhibited by in vitro methylation. Proc. Natl. Acad. Sci. USA 79, 1073-1077 (1982).
17. Busslinger, M., Hurst, J. & Flavell, R. A. DNA methylation and the regulation of globin gene expression. Cell 34, 107-206 (1983).
18. Simon, D. et al. Retroviral genomes methylated by mammalian but not bacterial methylase are non-infectious. Nature 304, 275-277 (1983).
19. Jang, S.K. & Wimmer, E. Cap-independent translation of encephalomyocarditis virus RNA: structural elements of the internal ribosomal entry site and involvement of a cellular 57-kd RNA-binding protein. Genes Dev. 4, 1560-1572 (1990).
20. Ure, J.M., Fiering, S. & Smith, A.G. A rapid and efficient method for freezing and recovering clones of embryonic stem cells. Trends Genet. 8, 6 (1992).
21. Herry, D.M., Connon, F. & Powell, R. Insert preparation by centrifugation through siliconized glass wool. Trends Genet. 6, 173 (1990).
22. Feinberg, A.P. & Vogelstein, B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 137, 266-267 (1984).
23. Sealey, P.G., Whittaker, P.A. & Southern, E.M. Removal of repeated sequences from hybridization probes. Nucl. Acids Res. 13, 1905-1922 (1985).
24. Ullrich, A. et al. Rat insulin genes: construction of plasmids containing the coding sequences. Science 196, 1313-1319 (1977).
25. Sambrook, J., Fritsch, E.F. & Maniatis, T. In Molecular Cloning, A Laboratory Manual 2nd ed. (Cold Spring Harbor Press, Cold Spring Harbor, NY, 1989).
26. Smith, A.G. et al. Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides. Nature 336, 688-690 (1988).
27. Whittingham, D.G. & Wales. R.G. Storage of two-cell mouse embryos in vitro. Aust. J. Biol. Sci. 22, 1065-1068 (1969).
28. Beddington, R.S.P., Morgenstern, J., Land, H. & Hogan, A. An in situ transgenic enzyme marker for the midgestation mouse embryo and the visualization of inner cell mass clones during early organogenesis. Development 106, 37-46 (1989).