Crystal structure of a methyltetrahydrofolate- and corrinoid-dependent methyltransferase

Structure

Volumn 8, Issue 8, 2000, Pages 817-830

  Doukov, Tzanko ^a   Seravalli, Javier ^a   Stezowski, John J ^a   Ragsdale, Stephen W ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

Author keywords

Carbon dioxide fixation; Cobalamin; Methyltetrahydrofolate; Methyltransferase; One carbon metabolism

Indexed keywords

5 METHYLTETRAHYDROFOLIC ACID; CORRINOID; DIHYDROPTEROATE SYNTHASE; METHYLTRANSFERASE; TRIOSEPHOSPHATE ISOMERASE;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CLOSTRIDIUM; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME STRUCTURE; MOORELLA THERMOACETICA; NONHUMAN; PRIORITY JOURNAL; PROTEIN TERTIARY STRUCTURE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS;

CLOSTRIDIUM; MOORELLA THERMOACETICA; POSIBACTERIA;

EID: 0034662752     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(00)00172-6     Document Type: Article

References (94)

1. The Eastern and Western branches of the Wood/Ljungdahl pathway: How the East and West were won
2. 12-dependent enzymes
3. The methanogenic corrinoid proteins
4. Studies on methyl chloride dehalogenase and O-demethylase in cell extracts of the homoacetogen strain MC based on a newly developed coupled enzyme assay
5. A corrinoid-dependent catabolic pathway for growth of a Methylobacterium strain with chloromethane
6. Formation of methylcoenzyme M from formaldehyde by cell-free extracts of Methanobacterium thermoautotrophicum. Evidence for the involvement of a corrinoid-containing methyltransferase
7. Methanol-coenzyme M methyltransferase from Methanosarcina barkeri. Zinc dependence and thermodynamics of the methanol:cob(I)alamin methyltransferase reaction
8. Reconstitution of monomethylamine-coenzyme M methyl transfer with a corrinoid protein and two methyltransferases purified from Methanosarcina barkeri
9. Clustered genes encoding the methyltransferases of methanogenesis from monomethylamine
10. Purification and characterization of dimethylamine: 5-hydroxybenzimidazolyl-cobamide methyltransferase from Methanosarcina barkeri Fusaro Eur
11. Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri
12. Tetramethylammonium:coenzyme M methyltransferase system from methanococcoides sp
13. Methylthiol-coenzyme M methyltransferase from Methanosarcina barkeri, an enzyme of methanogenesis from dimethylsulfide and methylmercaptopropionate
14. O-demethylase from Acetobacterium dehalogenans-cloning, sequencing, and active expression of the gene encoding the corrinoid protein
15. Unusual coenzymes of methanogenesis
16. Structural characteristics of methanogenic cofactors in the non-methanogenic archaebacterium Archaeoglobus fulgidus
17. Cofactor contents of methanogenic bacteria reviewed
18. The energy conserving N5-methyltetrahydromethanopterin-coenzyme M methyltransferase complex from Methanobacterium thermoautotrophicum is composed of eight different subunits
19. Cloning, sequencing and expression of the genes encoding the sodium translocating N-5-methyltetrahydromethanopterin:coenzyme M methyttransferase of the methylotrophic archaeon Methanosarcina mazei Go1
20. Sodium ion translocation by N5-methyltetrahydromethano-pterin:coenzyme M methyltransferase from Methanosarcina mazei Go1 reconstituted in ether lipid liposomes
21. Purification of five components from Clostridium thermoacticum which catalyze synthesis of acetate from pyruvate and methyltetrahydrofolate. Properties of phosphotransacetylase
22. The reductive acetyl coenzyme A pathway: Sequence and heterologous expression of active methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase from Clostridium thermoaceticum
23. Cloning and expression of the gene cluster encoding key proteins involved in acetyl-CoA synthesis in Clostridium thermoaceticum: CO dehydrogenase, the corrinoid/Fe-S protein, and methyltransferase
24. Mechanism of transfer of the methyl group from (6S)-methyltetrahydrofolate to the corrinoid/iron-sulfur protein catalyzed by the methyltransferase from Clostridium thermoaceticum: A key step in the Wood-Ljungdahl pathway of acetyl-CoA synthesis
25. 5 protonation of methyltetrahydrofolate in catalysis
26. Cobamide-dependent methyl transferases
27. Mechanistic studies of the methyltransferase from Clostridium thermoaceticum: Origin of the pH dependence of the methyl group transfer from methyltetrahydrofolate to the corrinoid/iron-sulfur protein
28. Acetate synthesis from carbon monoxide by Clostridium thermoaceticum. Purification of the corrinoid protein
29. Mossbauer, EPR, and optical studies of the corrinoid/Fe-S protein involved in the synthesis of acetyl-CoA by Clostridium thermoaceticum
30. Role of the [4Fe-4S] cluster in reductive activation of the cobalt center of the corrinoid iron-sulfur protein from Clostridium thermoaceticum during acetyl-CoA synthesis
31. The role of an iron-sulfur cluster in an enzymatic methylation reaction. Methylation of CO dehydrogenase/acetyl-CoA synthase by the methylated corrinoid iron-sulfur protein
32. Sequence and expression of the gene encoding the corrinoid/iron-sulfur protein from Clostridium thermoaceticum and reconstitution of the recombinant protein to full activity
33. Spectroelectrochemical studies of the corrinoid/iron-sulfur protein from Clostridium thermoaceticum
34. X-ray absorption spectroscopy of the corrinoid/iron sulfur protein involved in acetyl-CoA synthesis by Clostridium thermoaceticum
35. Structural and electronic factors in heterolytic cleavage: Formation of the Co(I) intermediate in the corrinoid/iron-sulfur protein from Clostridium thermoaceticum
36. Stereochemistry of acetic acid formation from 5-methyl-tetrahydrofolate by Clostridium thermoaceticum
37. Cobalamin-dependent methionine synthase is a modular protein with distinct regions for binding homocysteine, methyltetrahydrofolate, cobalamin, and adenosylmethionine
38. Cobalamin-dependent methionine synthase from Escherichia coli: Involvement of zinc in homocysteine activation
39. Characterization of the zinc binding site in methionine synthase enzymes of Escherichia coli: The role of zinc in the methylation of homocysteine
40. 12-binding domains of methionine synthase
41. 12-dependent enzymes
42. 12
43. Protein structural topology: Automated analysis and diagrammatic representation
44. Motif-based searching in TOPS protein topology databases
45. Classifying a protein in the CATH database of domain structures
46. SCOP: A structural classification of proteins database for the investigation of sequences and structures
47. Surprising similarities in structure comparison
48. Dali: A network tool for protein structure comparison
49. Crystal structure of the anti-bacterial sulfonamide drug target dihydropteroate synthase
50. Structure and function of the dihydropteroate synthase from Staphylococcus aureus
51. Structure of a hyperthermophilic tungstopterin enzyme, aldehyde ferredoxin oxidoreductase
52. + and biopterin
53. +-biopterin complexes
54. Crystal structures of recombinant human dihydrofolate reductase complexed with folate and 5-deazafolate
55. Direct measurement of the pK(a) of aspartic acid 26 in Lactobacillus casei dihydrofolate reductase: Implications for the catalytic mechanism
56. Crystal structure of a novel trimethoprim-resistant dihydrofolate reductase specified in Escherichia coli by R-plasmid R67
57. 12 radicals are generated: The crystal structure of methylmalonyl-coenzyme A mutase at 2 Å resolution
58. Conformational changes on substrate binding to methylmalonyl CoA mutase and new insights into the free radical mechanism
59. 12-dependent enzyme provides new mechanistic insights
60. 12 binding and the direct participation of a potassium ion in enzyme catalysis: X-ray structure of diol dehydratase
61. Theoretical studies on the activation of the pterin cofactor in the catalytic mechanism of dihydrofolate reductase
62. Mechanism of the reaction catalyzed by dihydrofolate reductase from Escherichia coli: pH and deuterium isotope effects with NADPH as the variable substrate
63. OLIGSCAN: A computer program to assist in the design of PCR primers homologous to multiple DNA sequences
64. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)
65. Preliminary X-ray diffraction analysis of the methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase from Clostridium thermoaceticum
66. Solvent content of protein crystals
67. Processing of X-ray diffraction data collected in oscilation mode
68. SHELX: Applications to macromolecules
69. Automated MAD and MIR structure solution
70. Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods
71. Methods used in the structure determination of bovine mitochondrial F1 ATPase
72. Crystallography and NMR system: A new software suite for macromolecular structure determination
73. XtalView/Xfit-A versatile program for manipulating atomic coordinates and electron density
74. SHELXL: High resolution refinement
75. Accurate bond and angle parameters for X-ray protein structure refinement
76. PROCHECK - A program to check stereochemical quality of protein structures
77. VERIFY3D: Assessment of protein models with three-dimensional profiles
78. GenBank
79. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
80. ClustalW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice
81. A workbench for multiple alignment construction and analysis
82. Pattern-induced multi-sequence alignment (PIMA) algorithm employing secondary structure-dependent gap penalties for use in comparative protein modelling
83. Protein structure comparison by alignment of distance matrices
84. Recent improvements of the ProDom database of protein domain families
85. Ribbons
86. Further additions to MolScript version 1.4, including reading and contouring of electron-density maps
87. Raster3D version 2.0. A program for photorealistic molecular graphics
88. ESPript: Multiple sequence alignments in PostScript