Single-molecule fluorescence detection of green fluorescence protein and application to single-protein dynamics

Methods in Cell Biology

Volumn , Issue 58, 1999, Pages 49-73

  Pierce, Daniel W ^a   Vale, Ronald D ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; PHOTOPROTEIN;

ANIMAL; FLUORESCENCE; FLUORESCENCE MICROSCOPY; METHODOLOGY; REVIEW;

ANIMALS; FLUORESCENCE; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE;

EID: 0032607868     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Article

References (33)

1. Axelrod, D. (1989). Total internal reflection fluorescence microscopy. Meth. Cell Biol. 30, 245-270.
2. Betzig, E., and Chichester, R. J. (1993). Single molecules observed by near-field scanning optical microscopy. Science 262, 1422-1428.
3. Block, S. M., Goldstein, L. S., and Schnapp, B. J. (1990). Bead movement by single kinesin molecules studied with optical tweezers. Nature 348, 348-352.
4. Brejc, K., Sixma, T. K., Kitts, P. A., Kain, S. R., Tsien, R. Y., Ormo, M., and Remington, S. J. (1997). Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 94, 2306-2311.
5. Capson, T. L., Benkovic, S. J., and Nossal, N. G. (1991). Protein-DNA cross-linking demonstrates stepwise ATP-dependent assembly of T4 DNA polymerase and its accessory proteins on the primer-template. Cell 65, 249-258.
6. Case, R. B., Pierce, D. W., Hom-Booher, N., Hart, C. L., and Vale, R. D. (1997). The directional preference of kinesin motors is specified by an element outside the motor catalytic domain. Cell 90, 959-966.
7. Chattoraj, M., King, B. A., Bublitz, G. U., and Boxer, S. G. (1996). Ultra-fast excited state dynamics in green fluorescent protein: multiple states and proton transfer. Proc. Natl. Acad. Sci. U. S. A. 93, 8362-8367.
8. Coppin, C., Finer, J., Spudich, J. S., and Vale, R. D. (1995). Measurement of the isometric force exerted by a single kinesin molecule. Biophys. J. 68, 242S-244S.
9. Cormack, B. P., Valdivia, R. H., and Falkow, S. (1996). FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173, 33-38.
10. Dickson, R. M., Cubitt, A. B., Tsien, R. Y., and Moerner, W. E. (1997). On/off blinking and switching behaviour of single molecules of green fluorescent protein. Nature 388, 355-358.
11. Enderle, T., Ha, T., Ogletree, D. F., Chemla, D. S., Magowan, C., and Weiss, S. (1997). Membrane specific mapping and colocalization of malarial and host skeletal proteins is the Plasmodium falciparum infected erythrocyte by dual-color near-field scanning optical microscopy. Proc. Natl. Acad. Sci. U. S. A. 94, 520-525.
12. Finer, J. T., Simmons, R. M., and Spudich, J. A. (1994). Single myosin molecule mechanics: piconewton forces and nanometre steps. Nature 368, 113-119.
13. Funatsu, T., Harada, Y., Tokunaga, M., Saito, K., and Yanagida, Y. (1995). Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution. Nature 374, 555-559.
14. Ha, T., Enderle, T., Ogletree, D. F., Chemla, D. S., Selvin, P. R., and Weiss, S. (1996). Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor. Proc. Natl. Acad. Sci. U. S. A. 93, 6264-6268.
15. Ha, T., Chemla, D. S., Enderle, T., and Weiss, S. (1997). Single molecule spectroscopy with automated positioning. Appl. Phys. Lett. 70, 782-784.
16. Harada, Y., Sakurada, K., Aoki, T., Thomas, D. D., and Yanagida, T. (1990). Mechanochemical coupling in actomyosin energy transduction studied by in vitro movement assay. J. Mol. Biol. 216, 49-68.
17. Heim, R., Prasher, D. C., and Tsien, R. Y. (1994). Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 91, 12501-12504.
18. Heim, R., Cubitt, A. B., and Tsien, R. Y. (1995). Improved green fluorescence. Nature 373, 663-664.
19. Howard, J., Hudspeth, A. J., and Vale, R. D. (1989). Movement of microtubules by single kinesin molecules. Nature 342, 154-158.
20. Ishijima, A., Harada, Y., Kojima, H., Funatsu, T., Higuchi, H., and Yanagida, T. (1994). Single-molecule analysis of the actomyosin motor using nanomanipulation. Biochem. Biophys. Res. Commun. 199, 1057-1063.
21. Iwane, A. H., Funatsu, T., Harada, Y., Tokunaga, M., Ohara, O., Morimoto, S., and Yanagida, T. (1997). Single molecular assay of individual ATP turnover by a myosin-GFP fusion protein expressed in vitro. FEBS Lett. 407, 235-238.
22. Lu, H. P., and Xie, X. S. (1997). Single-molecule spectral fluctuations at room temperature. Nature 385, 143-146.
23. Macklin, J. J., Trautman, J. K., Harris, T. D., and Brus, L. E. (1996). Imaging and time-resolved spectroscopy of single molecules at an interface. Science 272, 255-258.
24. Meyhofer, E., and Howard, J. (1995). The force generated by a single kinesin molecule against an elastic load. Proc. Natl. Acad. Sci. U. S. A. 92, 574-578.
25. Moerner, W. E., and Kador, L. (1989). Optical detection and spectroscopy of single molecules in a solid. Phys. Rev. Lett. 62, 2535-2538.
26. Neher, E., and Sakmann, B. (1976). Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature 260, 799-802.
27. Ormo, M., Cubitt, A. B., K., K., Gross, L. A., Tsien, R. Y., and Remington, S. J. (1996). Crystal structure of the Aequorea victoria green fluorescent protein. Science 273, 1392-1395.
28. Pierce, D. W., Hom-Booher, N., and Vale, R. D. (1997). Imaging individual green fluorescent proteins. Nature 388, 338.
29. Pierce, D. W., and Vale, R. D. (1998). Preparation and assay of fluorescent kinesin. Meth. Enzymol., Molecular Motors and the Cytoskeleton, Part B (in press).
30. Prasher, D. C., Eckenrode, V. K., Ward, W. W., Prendergast, F. G., and Cormier, M. J. (1992). Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111, 229-233.
31. Svoboda, K., Schmidt, C. F., Schnapp, B. J., and Block, S. M. (1993). Direct observation of kinesin stepping by optical trapping interferometry. Nature 365, 721-727.
32. Vale, R. D., Reese, T. S., and Sheetz, M. P. (1985). Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility. Cell 42, 39-50.
33. Vale, R. D., Funatsu, T., Pierce, D. W., Romberg, L., Harada, Y., and Yanagida, T. (1996). Direct observation of single kinesin molecules moving along microtubules. Nature 380, 451-453.