Reduced mobility of the alternate splicing factor (ASF) through the nucleoplasm and steady state speckle compartments

Journal of Cell Biology

Volumn 150, Issue 1, 2000, Pages 41-51

  Kruhlak, Michael J ^a   Lever, Melody A ^b   Fischle, Wolfgang ^c   Verdin, Eric ^c   Bazett Jones, David P ^a   Hendzel, Michael J ^b  

^a UNIVERSITY OF CALGARY   (Canada)

^b UNIVERSITY OF ALBERTA   (Canada)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

ASF SF2; Cell nucleus; FRAP; IGCs; Nuclear matrix

Indexed keywords

GREEN FLUORESCENT PROTEIN; NUCLEAR FACTOR; PHOSPHOTRANSFERASE INHIBITOR; RNA POLYMERASE;

ARTICLE; BINDING SITE; BLEACHING; CELL COMPARTMENTALIZATION; CELL NUCLEUS; CELL NUCLEUS MATRIX; DIFFUSION; HUMAN; MOLECULAR DYNAMICS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; STEADY STATE;

ANIMALS; BINDING SITES; BIOLOGICAL TRANSPORT; CELL COMPARTMENTATION; CELL LINE; CELL NUCLEUS; DIFFUSION; ENZYME INHIBITORS; FLUORESCENCE; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MICE; MUNTJACS; NUCLEAR PROTEINS; PHOTOCHEMISTRY; PROTEIN KINASE INHIBITORS; RECOMBINANT FUSION PROTEINS; RNA POLYMERASE II; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

EID: 0034631851     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.150.1.41     Document Type: Article

References (32)

1. Carmo-Fonseca, M., C. Cunha, N. Custodio, C. Carvalho, P. Jordan, J. Ferreira, and L. Parreira. 1996. The topography of chromosomes and genes in the nucleus. Exp. Cell Res. 229:247-252.
2. Cremer, T., A. Kurz, R. Zirbel, S. Dietzel, B. Rinke, E. Schrock, M.R. Speicher, U. Mathieu, A. Jauch, P. Emmerich. et al. 1993. Role of chromosome territories in the functional compartmentalization of the cell nucleus. Cold Spring Harb. Symp. Quant. Biol. 58:777-792.
3. de Jong, L., M.A. Grande, K.A. Mattern, W. Schul, and R. van Driel. 1996. Nuclear domains involved in RNA synthesis, RNA processing, and replication. Crit. Rev. Eukaryot. Gene Expr. 6:215-246.
4. Fay, F.S., K.L. Taneja, S. Shenoy, L. Lifshitz, and R.H. Singer. 1997. Quantitative digital analysis of diffuse and concentrated nuclear distributions of nascent transcripts, SC35 and poly(A). Exp. Cell Res. 231:27-37.
5. Fejesw-Toth, G., D. Pearce, and A. Naray-Fejes-Toth. 1998. Subcellular localization of mineralocorticoid receptors in living cells: effects of receptor agonists and antagonists. Proc. Natl. Acad. Sci. USA. 95:2973-2978.
6. Fischle, W., S. Emiliani, M.J. Hendzel, T. Nagase, N. Nomura, W. Voelter, and E. Verdin. 1999. A new family of human histone deacetylases related to Saccharomyces cerevisiae HDA1p. J. Biol. Chem. 274:11713-11720.
7. Hendzel, M.J., and D.P. Bazett-Jones. 1995. RNA polymerase II transcription and the functional organization of the mammalian cell nucleus. Chromosoma. 103:509-516.
8. Hendzel, M.J., M.J. Kruhlak, and D.P. Bazett-Jones. 1998. Organization of highly acetylated chromatin around sites of heterogeneous nuclear RNA accumulation. Mol. Biol. Cell. 9:2491-25117.
9. Hendzel, M.J., F. Boisvert, and D.P. Bazett-Jones. 1999. Direct visualization of a protein nuclear architecture. Mol. Biol. Cell. 10:2051-2062.
10. Htun, H., J. Barsony, I. Renyi, D.L. Gould, and G.L. Hager. 1996. Visualization of glucocorticoid receptor translocation and intranuclear organization in living cells with a green fluorescent protein chimera. Proc. Natl. Acad. Sci. USA. 93:4845-4850.
11. Huang, S., and D.L. Spector. 1996. Intron-dependent recruitment of pre-mRNA splicing factors let sites of transcription. J. Cell Biol. 133:719-732.
12. Jackson, D.A. 1997. Chromatin domains and nuclear compartments: establishing sites of gene expression in eukaryotic nuclei. Mol. Biol. Rep. 24:209-220.
13. Khodjakov, A., and C.L. Rieder, 1999. The sudden recruitment of γ-tubulin to the centrosome at the onset of mitosis and its dynamic exchange throughout the cell cycle, do not require microtubules. J. Cell Biol. 146:585-596.
14. Lamond, A.I., and W.C. Earnshaw. 1998. Structure and function in the nucleus. Science. 280:547-553.
15. Misteli, T. 2000. Cell biology of transcription and pre-mRNA splicing: nuclear architecture meets nuclear functions. J. Cell Sci. 113:1841-1849.
16. Misteli, T., and D.L. Spector. 1996. Serine/threonine phosphatase I modulates the subnuclear distribution of pre-mRNA splicing factors. Mol. Biol. Cell. 7:1559-1572.
17. Misteli, T., and D.L. Spector. 1998. The cellular organization of gene expression. Curr. Opin. Cell Biol. 10:323-331.
18. Misteli, T., J.F. Caceres, and D.L. Spector. 1997. The dynamics of a pre-mRNA splicing factor in living cells. Nature. 387:523-527.
19. Misteli, T., J.F. Caceres, J.Q. Clement, A.R. Krainer, M.F. Wilkinson, and D.L. Spector. 1998. Serine phosphorylation of SR proteins is required for their recruitment to sites of transcription in vivo. J. Cell Biol. 143:297-307.
20. Nakata, T., S. Terada, and M. Hirokawa. 1998. Visualization of the dynamics of synaptic vesicle and plasma membrane proteins in living axons. J. Cell Biol. 140:659-674.
21. Pederson, T. 1998. Thinking about a nuclear matrix. J. Mol. Biol. 277:147-159.
22. Phair, R.D., and T. Misteli. 200. High mobility of proteins in the cell nucleus. Nature. 404:604-609.
23. Politz, J.C., E.S. Browne, D.E. Wolf, and T. Pederson. 1998. Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells. Proc. Natl. Acad. Sci. USA. 95:6043-6048.
24. Seksek, O., J. Biwersi, and A.S. Verkman. 1997. Translational diffusion of macromolecule-sized solutes in the cytoplasm and nucleus. J Cell Biol. 138:131-142.
25. Singer, R.H., and M.R. Green. 1997. Compartmentalization of eukaryotic gene expression: causes and effects. Cell. 91:291-294.
26. Sleeman, J.E., and A.I. Lamond. 1999. Nuclear organization of pre-mRNA splicing factors. Curr. Opin. Cell Biol. 11:372-377.
27. Sleeman, J., C.B. Lyon, M. Platani, J.P. Kreivi, and A.I. Lamond. 1998. Dynamic interactions between splicing snRNPs, coiled bodies and nucleoli revealed using snRNP protein fusions to the green fluorescent protein. Exp. Cell Res. 243:290-304.
28. Spector, D.L. 1993. Nuclear organisation of pre-mRN A processing. Curr. Opin. Cell Biol. 5:442-147.
29. Spector, D.L., X.D. Fu, and T. Maniatis. 1991. Association between distinct pre-mRNA splicing components and the cell nucleus. EMBO (Eur. Mol. Biol. Organ.) J. 10:3467-3481.
30. Wei, X., S. Somanathan, J. Samarabandu, and R. Berezney. 1999. Three-dimensional visualization of transcription sites and their association with splicing factor-rich nuclear speckles. J Cell Biol. 146:543-558.
31. White, J., and E. Stelzer. 1999. Photobleaching GFP reveals protein dynamics inside living cells. Trends Cell Biol. 9:61-65.
32. Zirbel, R.M., U.R. Mathieu, A. Kurz, T. Cremer, and P. Lichter. 1993. Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries. Chromosome Res. 1:93-106.