In vitro and in vivo evidence for the long-term multilineage (myeloid, B, NK, and T) reconstitution capacity of ex vivo expanded human CD34+ cord blood cells

Experimental Hematology

Volumn 28, Issue 12, 2000, Pages 1470-1480

  Kobari, Ladan ^a   Pflumio, Françoise ^b   Giarratana, Marie Catherine ^a   Li, Xiaxin ^c   Titeux, Monique ^b   Izac, Brigitte ^b   Leteurtre, François ^c   Coulombel, Laure ^b   Douay, Luc ^a,d  

^a HÔPITAL SAINT ANTOINE   (France)

^b INSTITUT GUSTAVE ROUSSY   (France)

^c SAINT LOUIS HOSPITAL   (France)

^d ARMAND TROUSSEAU HOSPITAL   (France)

Author keywords

B lymphopoiesis; CD34+ cord blood cells; Ex vivo expansion; Myelopoiesis assays; NK lymphopoiesis; NOD SCID; T lymphopoiesis

Indexed keywords

CD34 ANTIGEN; CYTOKINE; ERYTHROPOIETIN; GRANULOCYTE COLONY STIMULATING FACTOR; MEGAKARYOCYTE GROWTH AND DEVELOPMENT FACTOR; RECOMBINANT GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; STEM CELL FACTOR; TELOMERASE;

ARTICLE; CELL PROLIFERATION; COLONY FORMING UNIT; CONTROLLED STUDY; FLOW CYTOMETRY; HEMATOPOIETIC CELL; HUMAN; HUMAN CELL; LYMPHOPOIESIS; MYELOPOIESIS; PRIORITY JOURNAL; TELOMERE; UMBILICAL CORD BLOOD;

ANIMALS; ANTIGENS, CD34; B-LYMPHOCYTES; CELL DIFFERENTIATION; CELLS, CULTURED; FETAL BLOOD; GRAFT SURVIVAL; GRANULOCYTE COLONY-STIMULATING FACTOR; GRANULOCYTES; HEMATOPOIESIS; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HEMATOPOIETIC STEM CELLS; HUMANS; KILLER CELLS, NATURAL; LYMPHOCYTES; MEMBRANE PROTEINS; MICE; MICE, INBRED NOD; MICE, SCID; STEM CELL FACTOR; T-LYMPHOCYTES; THROMBOPOIETIN;

EID: 0034536589     PISSN: 0301472X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0301-472X(00)00557-9     Document Type: Article

References (52)

1. Treatment of genetic defects in hematopoietic cell function by gen transfer
2. Ex vivo expansion of hematopietic precursors, progenitors, and stem cells; the next generation of cellular therapeutics
3. New developments in hematopoietic stem cell expansion
4. + cell selection in stroma-free cultures
5. Cell culture bags allow a large extent of ex vivo expansion of LTC-IC and functional mature cells which can subsequently be frozen; interest for a large-scale clinical applications
6. Loss of long-term repopulating ability in long term bone marrow culture
7. EPO, G-CSF, FL and MGDF enhance ex vivo expansion of hematopoietic cell compartments in presence of SCF, IL3 and IL6
8. Self-renewal of primitive human hematopoietic cells (long-term-culture-initiating cells) in vitro and their expansion in defined medium
9. Thrombopoietin, Kit ligand, and flk2/flt3 ligand together induce increased numbers of primitive hematopoietic progenitors from human CD34+Thy1+Lin- cells with preserved ability to engraft SClD-hu bone
10. + cells and recruit primitive CD34+Thy-1+ cells into rapid division
11. Presence of primitive lymphoid progenitors with NK or B potential in ex vivo expanded bone marrow cell cultures
12. Cord-blood transplants
13. Unrelated mismatched cord blood transplantation in patient with hematological malignancies; a single institution experience
14. Murine marrow cells expanded in culture with IL-3, IL-6, IL-11, and SCF acquire an engraftment defect in normal hosts
15. Ex vivo expansion of murine hematopoietic progenitor cells generates classes of expanded cells possessing different levels of bone marrow repopulating potential
16. + cells in serum-free cultures containing steel factor, interleukin-6, and erythropoietin with maintenance of cells with long-term in vivo reconstituting potential
17. Expansion in vitro of transplantable human cord blood stem cells demonstrated using a quantitative assay of their lympho-myeloid repopulating activity in nonobese diabetic scid/scid mice
18. Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice
19. + cord blood cells
20. + cord blood cells after ex vivo expansion; evidence for the amplification and self-renewal of repopulating stem cells
21. Expansion of human NOD/SCID-repopulating cells by stem cell factor, Flke/Flt3 ligand, thrombopoietine, IL-6, and soluble IL-6 receptor
22. Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive marrow stromal layers
23. Extended long-term culture reveals a highly quiescent and primitive human hematopoietic progenitor population
24. Telomere length regulation
25. Interleukin-2 interacts with myeloid growth factors in serum-free long-term bone marrow culture
26. Phenothype and function of human hematopoietic cells engrafting immune-deficient CB17-severe combined immunodeficiency mice and nonobese diabetic-severe combined immunodefiency mice after transplantation of human cord blood mononuclear cells
27. + NK progenitor
28. Differentiation of human CD34+CD38- cord blood stem cells into B cell progenitors in vitro
29. Identification of lymphomyeloid primitive progenitor cells in fresh human cord blood and in the marrow of nonobese diabetic-severe combined immunodeficient (NOD-SCID) mice transplanted with human CD34+ cord blood cells
30. + subsets of human cord blood and bone marrow cells using NOD-SCID fetal thymus organ cultures
31. Evidence for a mitotic clock in human hematopoietic stem cells; loss of telomeric DNA with age
32. + enriched cells from umbilical cord blood in NOD/LtSz-scid/scid mice
33. Telomerase regulation, cell cycle, and telomere stability in primitive hematopoietic cells
34. Telomerase activity in candidate stem cells from fetal liver and adult bone marrow
35. Cytokine manipulation of primitive human hematopoietic cell self-renewal
36. low/- cells generated in a xenogenic coculture system are capable of both long-term hematopoiesis and multiple differentiation
37. - cells and maintains progenitor cells in vitro
38. Differential cytokines effects on primitive (CD34+CD38-) human hematopoietic cells; novel responses to Flt3-Ligand and thrombopoietin
39. Distinct requirements for optimal growth and In vitro expansion of human CD34(+)CD38(-) bone marrow long-term culture-initiating cells (LTC-IC), extended LTC-IC, and murine in vivo long-term reconstituting stem cells
40. - cells in cord blood and bone marrow
41. Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow; implication for gene therapy
42. Sustained proliferation, multi-lineage differentiation and maintenance of primitive human haemopoietic cells in NOD/SCID mice transplanted with human cord blood
43. - progenitor cells from human cord blood generate B lymphocytes and granulocytes
44. Identification of a novel human thymocyte subset with a phenotype of CD3-CD4+ CD8α+β-1: Possible progeny of the CD 8
45. Differentiation of CD3-4-8- human fetal thymocytes in vivo; characterization of a CD3-4+8- intermediate
46. Cytokine stimulation of multilineage hematopoiesis from immature human cells engrafted in SCID mice
47. Reconstitution of SCID mice with human lymphoid and myeloid cells after transplantation with human fetal bone marrow without the requirement for exogenous human cytokines
48. Thymic repopulation by CD34(+) human cord blood cells after expansion in stroma-free culture
49. Accelerated telomere shortening in bone marrow recipients
50. - cells; correlation between long-term culture-initiating cells and telomerase expression
51. Engraftment of ex vivo-expanded cord blood cells in NOD/SCID mice; what is the clinical relevance?
52. Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4