Lentivirus gene transfer in murine hematopoietic progenitor cells is compromised by a delay in proviral integration and results in transduction mosaicism and heterogeneous gene expression in progeny cells

Journal of Virology

Volumn 74, Issue 24, 2000, Pages 11911-11918

  Mikkola, H ^a   Woods, N B ^a   Sjogren M ^a   Helgadottir, H ^a   Hamaguchi, I ^a   Jacobsen, S E ^a   Trono, D ^a   Karlsson, S ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; INTERLEUKIN 3; INTERLEUKIN 6; METHYLCELLULOSE; STEM CELL FACTOR; VIRUS VECTOR;

ANIMAL CELL; ARTICLE; CELL DIVISION; CELL PROLIFERATION; CONTROLLED STUDY; GENE THERAPY; GENE TRANSFER; GENETIC TRANSDUCTION; HEMATOPOIETIC STEM CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; LENTIVIRINAE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROVIRUS; VESICULAR STOMATITIS VIRUS; VIRUS DNA CELL DNA INTERACTION; VIRUS GENOME;

ANIMALS; GENE EXPRESSION REGULATION, VIRAL; GENES, VIRAL; HEMATOPOIETIC STEM CELLS; LENTIVIRUS; MICE; TIME FACTORS; TRANSFECTION; VIRUS INTEGRATION;

EID: 0034469216     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.74.24.11911-11918.2000     Document Type: Article

References (46)

1. + cells with a human immunodeficiency virus type 1-based retroviral vector pseudotyped with vesicular stomatitis virus envelope glycoprotein G
2. Highly efficient and sustained gene transfer in adult neurons with a lentivirus vector
3. Interaction of human immunodeficiency virus-derived vectors with wild-type virus in transduced cells
4. A nuclear localization signal within HIV-1 matrix protein that governs infection of non-dividing cells
5. - human hematopoietic cells by HIV-1-based lentiviral vectors
6. Green fluorescent protein as a marker for gene expression
7. Use of green fluorescent protein variants to monitor gene transfer and expression in mammalian cells
8. A GFP reporter system to assess gene transfer and expression in human hematopoietic progenitor cells
9. Retroviral vector design for long-term expression in murine hematopoietic cells in vivo
10. Self-inactivating lentiviral vector with enhanced transgene expression as potential gene transfer system in Parkinson's disease
11. + cells via human immunodeficiency virus-based gene transfer vectors
12. A third-generation lentivirus vector system with a conditional packaging system
13. - cell transduction via lentivirus-based gene transfer vectors
14. Low levels of deoxynucleotides in peripheral blood lymphocytes: A strategy to inhibit human immunodeficiency virus type 1 replication
15. Infection of intact human islets by a lentiviral vector
16. Transgene expression in the guinea pig cochlea mediated by a lentivirus-derived gene transfer vector
17. Deoxyribonucleoside triphosphate pool imbalances in vivo are associated with an increased retroviral mutation rate
18. Sustained expression of genes delivered directly into liver and muscle by lentiviral vectors
19. Lentiviral gene transfer to the nonhuman primate brain
20. 1b phase of the cell cycle is required for completion of human immunodeficiency virus type 1 reverse transcription in T cells
21. 0 lymphocytes
22. Passage through mitosis is required for oncoretroviruses but not for the human immundeficiency virus
23. Characterization of membrane components of the erythrocyte involved in vesicular stomatitis virus attachment and fusion at acidic pH
24. Restriction and enhancement of human immunodeficiency virus type 1 replication by modulation of intracellular deoxynucleoside triphosphate pools
25. Viral vectors for gene therapy of hematopoietic cells
26. Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection
27. Stable integration of human immunodeficiency virus-based retroviral vectors into the chromosomes of non-dividing cells
28. Stable and efficient gene transfer into the retina using an HIV-based lentiviral vector
29. Development of self-inactivating lentivirus vector
30. + cells that mediate long-term engraftment of NOD/SCID mice by HIV vectors
31. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector
32. Lentiviral vectors
33. A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes
34. Efficient lentiviral transduction of liver requires cell cycling in vivo
35. Primary structure of the Aequorea victoria green fluorescent protein
36. Transduction of nondividing cells using pseudotyped defective high-titer HIV type 1 particles
37. HIV-replication is controlled at the level of T cell activation and proviral integration
38. Human immunodeficiency virus type 1 vectors efficiently transduce human hematopoietic stem cells
39. Transduction of human progenitor hematopoietic cells stem cells by human immunodeficiency virus type 1-based vectors is cell cycle dependent
40. 1 human hematopoietic stem cells
41. Efficient and sustained transgene expression in human corneal cells mediated by a lentiviral vector
42. HIV-1 genome nuclear import is mediated by a central DNA flap
43. Increasing transduction efficiency of recombinant murine retrovirus vectors by initiation of endogenous reverse transcription: Potential utility for genetic therapies
44. Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo
45. Self-inactivating lentivirus vectors for safe and efficient in vivo gene delivery
46. Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors