Efficient and sustained transgene expression in human corneal cells mediated by a lentiviral vector

Gene Therapy

Volumn 7, Issue 3, 2000, Pages 196-200

  Wang, X ^a   Appukuttan, B ^a   Ott, S ^a   Patel, R ^a   Irvine, J ^b   Song, J ^b   Park, J H C ^a   Smith, R ^b   Stout, J T ^a  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^b DOHENY EYE INSTITUTE   (United States)

Author keywords

Cornea; Corneal endothelium; Corneal epithelium; Gene transfer; Lentiviral vector; Transduction

Indexed keywords

GREEN FLUORESCENT PROTEIN; VIRUS VECTOR;

ARTICLE; CELL CULTURE; CORNEA CELL; CORNEA STROMA; ENDOTHELIUM CELL; EPITHELIUM CELL; FLUORESCENCE ACTIVATED CELL SORTER; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; HUMAN TISSUE; LENTIVIRINAE; NONHUMAN; ORGAN CULTURE; PENETRATING KERATOPLASTY; PRIORITY JOURNAL; TRANSGENE; VIRUS RECOMBINANT;

EID: 0033994663     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301075     Document Type: Article

References (24)

1. Oral HB et al. Ex vivo adenovirus-mediated gene transfer and immunomodulatory protein production in human cornea. Gene Therapy 1997; 4: 639-647.
2. Fehervari Z et al. Gene transfer to ex vivo stored corneas. Cornea 1997; 16: 459-464.
3. Larkin DFP et al. Adenovirus-mediated gene delivery to the corneal endothelium. Transplantation 1996; 61: 363-370.
4. Budenz DL, Bennett J, Alonso L, Maguire A. In vivo gene transfer into murine corneal endothelial and trabecular meshwork cells. Invest Ophthalmol Vis Sci 1995; 36: 2211-2215.
5. Oshima Y et al. Targeted gene transfer to corneal endothelium in vivo by electric pulse. Gene Therapy 1998; 5: 1347-1354.
6. Wang XG et al. Non-viral vector transfection efficiency with ocular and vascular cells (submitted for publication).
7. Bukrinsky MI et al. A nuclear localization signal within HIV-1 matrix protein that governs infection of non-dividing cells. Nature 1993; 365: 666-669.
8. Schwedler UV, Kornbluth RS, Trono D. The nuclear localization signal of the matrix protein of human immunodeficiency virus type 1 allows the establishment of infection in macrophages and quiescent T lymphocytes. Proc Natl Acad Sci USA 1994; 91: 6992-6996.
9. Burns JC et al. Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and non-mammalian cells. Proc Natl Acad Sci USA 1993; 90: 8033-8037.
10. Gallardo HF, Tan C, Ory D, Sadelain M. Recombinant retroviruses pseudotyped with the vesicular stomatitis virus G glycoprotein mediate both stable gene transfer and pseudotransduction in human peripheral blood lymphocytes. Blood 1997; 90: 952-957.
11. Uchida N et al. HIV, but not leukemia virus, vectors mediate high efficiency gene transfer into freshly isolated G0/G1 human hematopoietic stem cells. Proc Natl Acad Sci USA 1998; 95: 11939-11944.
12. - human hematopoietic cells by HIV-1 based lentiviral vectors. Proc Natl Acad Sci USA 1999; 96: 2988-2993.
13. Blömer U et al. Highly efficient and sustained gene transfer in adult neurons with a lentivirus vector. J Virol 1997; 71: 6641-6649.
14. Naldini L et al. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 1996; 272: 263-267.
15. Zufferey R et al. Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Nat Biotechnol 1997; 15: 871-875.
16. Kafri T et al. Sustained expression of genes delivered directly into liver and muscle by lentiviral vectors. Nat Genet 1997; 17: 314-317.
17. Miyoshi H, Takahashi M, Gage FH, Verma IM. Stable and efficient gene transfer into the retina using an HIV-based lentiviral vector. Proc Natl Acad Sci USA 1997; 94: 10319-10323.
18. Frueh BE, Bohnke M. Corneal grafting of donor tissue preserved for longer than 4 weeks in organ-culture medium. Cornea 1995; 14: 463-466.
19. Redbrake C, Sallas S, Frantz A. Changes in human donor corneas preserved for longer than 4 weeks. Cornea 1998; 17: 62-65.
20. Price FW, Whitson WE, Collins KS, Marks RG. Five-year corneal graft survival. Arch Ophthalmol 1993; 111: 799-805.
21. Vail A et al. Corneal transplantation in the United Kingdom and Republic of Ireland. Br J Ophthalmol 1993; 77: 650-652.
22. Bourne WM, Hodge DO, Nelson LR. Corneal endothelium five years after transplantation. Am J Ophthalmol 1994; 118: 185-196.
23. Schermer A, Galvin S, Sun T. Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J Cell Biol 1986; 103: 49-62.
24. Qavi HB et al. Morphological and ultrastructural changes induced in corneal epithelial cells by HIV-1 and HHV-6 in vitro. Curr Eye Res 1996; 15: 597-604.