Analysis of libraries encoded with GC tags: Compound elution, tag decode analysis, and statistical sampling analysis

Molecular Diversity

Volumn 5, Issue 1, 2000, Pages 35-49

  Dolle, Roland E ^a,d   Guo, Joan ^a   Li, Wenni ^a   Zhao, Ning ^b   Connelly, James A ^c  

^a PHARMACOPEIA INC   (United States)

^b BRISTOL MYERS SQUIBB   (United States)

^c Selectide Corporation   (United States)

^d ADOLOR CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RESIN;

ARTICLE; CHEMISTRY; MEDICAL LITERATURE; PRIORITY JOURNAL; QUALITY CONTROL; SAMPLING; STATISTICAL ANALYSIS;

CHROMATOGRAPHY, LIQUID; DRUG DESIGN; DRUG EVALUATION, PRECLINICAL; MASS SPECTROMETRY; OLIGONUCLEOTIDES; QUALITY CONTROL; SEQUENCE TAGGED SITES;

EID: 0034444228     PISSN: 13811991     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1011393414573     Document Type: Article

References (26)

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25. Parity tags are generally employed together with the synthon tag set to maintain the total tag count at an even number for any given set of encoded synthons. The parity tags assist in resolving the occasional tag ambiguity which arises from the GC auto scoring function.
26. The number of random beads that must be sampled from a given library to give a statistically meaningful result is equivalent to 10× the largest combined synthon set. For an account of the statistical sampling methodology see: Dolle, R.E., Guo, J., O'Brien, L., Jin, Y, Piznik, M., Bowman, K.L., Li, W., Egan, W.J., Cavallaro, C.L., Roughton, A.L., Zhao, Q., Reader, J.C., Orlowski, M., Jacob-Samuel, B. and Dilanni Carroll, C., A statistical-based approach to assessing the fidelity of combinatorial libraries encoded with electrophone molecular tags. Development and application of tag decodeassisted single bead LC/MS analysis. J. Combin. Chem., 2 (2000) 716-731.