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Volumn 39, Issue 17, 2000, Pages 3055-3059

Supramolecular nanocircles consisting of streptavidin and DNA

Author keywords

DNA hybridization; Nanostructures; Streptavidin; Supramolecular chemistry

Indexed keywords

DNA; OLIGOMER; STREPTAVIDIN;

EID: 0034283190     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/1521-3773(20000901)39:17<3055::aid-anie3055>3.0.co;2-r     Document Type: Article
Times cited : (45)

References (51)
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    • The addition of single-stranded binding protein (Promega) to the product mixture from the thermal denaturation of the oligomers 3 and subsequent electrophoretic analysis indicates the presence of free single-stranded DNA. Moreover, conjugates containing STV and one or more non-complementary single-stranded DNA molecules were determined to migrate as constituents of the diffuse background hand. The formation of the single-stranded species occurs due to the rapid cooling rates inhibiting quantitative intermolecular rehybridization.
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    • Temperature-dependent studies indicate that the displacement of the biotinylated dsDNA ligand in 3b by an 800-fold excess of D-biotin starts to occur by heating to about 70°C for two minutes. The displacement is half-completed at about 80°C. The denaturation of the dsDNA 1b occurs at 71 ±1°C and the preferred formation of the nanocircle 4b occurs at 79 ±2°C.
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    • Electrophoretic gel-shift analyses indicate that both low molecular weight biotin components, such as biotin-fluorescein conjugate, and biotinylated proteins, such as antibodies, bind to the nanocirclcs 4.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.