Quantitative fluorescence cytometric analysis of Bcl-2 levels in tumor cells exhibiting a wide range of inherent Bcl-2 protein expression: Correlation with Western blot analysis

Cytometry

Volumn 40, Issue 4, 2000, Pages 346-352

  Dragowska, Wieslawa H ^a   De Lopes Menezes, Daniel E ^a   Sartor, Jason ^a   Mayer, Lawrence D ^a,b  

^a BRITISH COLUMBIA CANCER AGENCY   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Bcl 2; Breast carcinoma cell lines; QFCM; Western blot analysis

Indexed keywords

PROTEIN BCL 2;

ANTIGEN BINDING; ARTICLE; BINDING SITE; BREAST CARCINOMA; CANCER CELL CULTURE; FLUORESCENCE MICROSCOPE; HUMAN; HUMAN CELL; IMMUNOBLOTTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION;

BLOTTING, WESTERN; BREAST NEOPLASMS; FEMALE; FLOW CYTOMETRY; GENE EXPRESSION; HUMANS; IMAGE CYTOMETRY; IMMUNOGLOBULIN G; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; PROTO-ONCOGENE PROTEINS C-BCL-2; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0034254977     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0320(20000801)40:4<346::AID-CYTO10>3.0.CO;2-W     Document Type: Article

References (25)

1. Investigation of the subcellular distribution of Bcl-2 oncoprotein: Residence in nuclear envelope, endoplasmic reticulum and outer mitochondrial membranes
2. Mitochondria and apoptosis
3. Mitotic chromosomal Bcl-2: I. Stable expression throughout the cell cycle and association with isolated chromosomes
4. Prolonged survival of B-lineage acute lymphoblastic leukemia cells is accompanied by overexpression of Bcl-2 protein
5. The Bcl-2 protein family: Arbiters of cell survival
6. Expression of apoptosis-controlling proteins in acute leukemia cells
7. Prognostic significance of Bcl-2 protein expression and Bcl-2 gene rearrangement in diffuse aggressive non-Hodgkin's lymphoma
8. What is the value of Bcl-2 detection for histopathologists?
9. Elevated Bcl-2/Bax are a consistent feature of apoptosis resistance in B-cell chronic lymphocytic leukemia and are correlated with in vivo chemoresistance
10. Synthesis of Bcl-2 in response to anthracycline treatment may contribute to an apoptosis-resistant phenotype in leukemic cell lines
11. Bcl-2 antisense therapy chemosensitizes human melanoma in SCID mice
12. Tumor regression of human breast carcinoma by combination therapy of anti-bcl-2 antisense oligonuclcotide and chemotherapeutic drugs
13. Antisense Bcl-2 oligodeoxynucleotides inhibit progression to androgen independence after castration in the Shionogi tumor model
14. Bcl-2 protein expression in normal human bone marrow precursors and in acute myelogenous leukemia
15. Multiparameter flow-cytometric analysis of Bcl-2 and Fas expression in normal and neoplastic hematopoiesis
16. Expression of Bcl-2 proto-oncogene in adult acute lymphoblastic leukemia
17. Flow cytometric analysis of apoptosis and Bcl-2 in human solid neoplasms
18. Flow cytometric analysis of apoptosis and Bcl-2 in primary breast carcinomas: Clinical and biological implications
19. Standardizing flow cytometry: A classification system of fluorescence standards used for flow cytometry
20. Flow cytometric quantitation of immunofluorescence intensity: Problems and perspectives
21. Performance of calibration standards for antigen quantitation with flow cytometry
22. + T lymphocytes as a biological calibrator
23. Determination of CD4 antigen density on cells: Role of antibody valency, avidity, clones and conjugation
24. Molecular quantification of cell cycle-related gene expression at the protein level
25. Analysis of intracellular proteins