Molecular quantification of cell cycle-related gene expression at the protein level

Cytometry

Volumn 39, Issue 1, 2000, Pages 79-89

  Frisa, Phyllis S ^a   Lanford, Robert E ^c   Jacobberger, James W ^a,b  

^a CASE WESTERN RESERVE UNIVERSITY   (United States)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c SOUTHWEST FOUNDATION FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

Flow microfluorimetry; Fluorescence; Immunoblot; Quantitative immunofluorescence; Standardization; SV40 large T antigen

Indexed keywords

VIRUS LARGE T ANTIGEN;

ANIMAL CELL; ARTICLE; CELL CYCLE; CONTROLLED STUDY; FLOW CYTOMETRY; GENE EXPRESSION; IMMUNOFLUORESCENCE; MICROFLUOROMETRY; MOUSE; NEWBORN; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY; WESTERN BLOTTING;

EID: 0033989057     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(20000101)39:1<79::AID-CYTO11>3.0.CO;2-3     Document Type: Article

References (42)

1. Sladek TL, Jacobberger JW. Dependence of SV40 large T-antigen cell cycle regulation on T-antigen expression levels. Oncogene 1992;7: 1305-1313
2. Sladek TL, Jacohberger JW. Flow cytometric titration of retroviral expression vectors: comparison of methods for analysis of immunofluorescence histograms derived from cells expressing low antigen levels. Cytometry 1993;14:23-31.
3. Lenkei R, Mandy F, Marti G, Vogt R. Quantitative fluorescence cytometry: an emerging consensus - preface. Cytometry 1998;33:93.
4. Engelhard HH III, Krupka JL, Bauer KD. Simultaneous quantification of c-myc oncoprotein, total cellular protein, and DNA content, using multiparamter flow cytometry. Cytometry 1991;12:68-76.
5. Sladek TL, Fisher SD, Rubenstein BN. Dose-response of two cellular proliferation phenotypes produced by simian virus 40 large T antigen. Cell Prolif 1994;27:711-721.
6. He L, Fox MH. Comparison of flow cytometry and western blotting to measure Hsp70. Cytometry 1996;25:280-286.
7. Jacobberger JW, Fogleman D, Lehman JM. Analysis of intracellular antigens by flow cytometry. Cytometry 1986;7:356-364.
8. Frisa PS, Goodman MN, Smith GM, Silver J, Jacobberger JW. Immortalization of immature and mature astrocytes by SV40 T antigen. J Neurosci Res 1994;39:47-56.
9. Lanford R. Expression of simian virus 40 T antigen in insect cells using a baculovirus expression vector. Virology 1988;167:72-81.
10. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-685.
11. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979;76:4350-4354.
12. Staufenbiel M, Deppert W. Different structural systems of the nucleus are targets for SV40 large T antigen Cell 1983;33:173-181.
13. Djelloul S, Forgue-Lafitte M-E, Hermelin B, Marcel M. Bruyncel E. Baldi A, Giordano A, Chastre E, Gespach C. Enterocyte differentiation is compatible with SV40 large T expression and loss of p53 function in human colonic Caco-2 cells. FEBS Lett 1997;406:234-242.
14. Shibata M-A, Maroulakou IG, Jorcyk CL, Gold LG, Ward JM, Green JE. p53-independent apoptosis during mammary tumor progression in C3(1)/SV40 large T antigen transgenic mice: suppression of apoptosis during the transition from preneoplasia to carcinoma. Cancer Res 1996;56:2998-3003.
15. Martin D, Ruther U, Sanchez-Sweatman O, Orr F, Khokha R. Inhibition of SV40 antigen-induced hepatocellular carcinoma in TIMP-1 transgenic mice. Oncogene 1996;13:569-576.
16. Albright CD, Liu R, Bethea TC, Da Costa K-A, Salganik RI, Zeisel SH. Choline deficiency induces apoptosis in SV40-immortalized CWSV-1 rat hepatocytes in culture. FASEB J 1996;10:510-516.
17. Montenarh M, Quaiser A. Two different protein-protein interactions in oligomeric complexes of SV40 large T antigen with the cellular oncoprotein p53. Oncogene 1989;4:379-382.
18. Staufenbiel M, Deppert W. Preparation of nuclear matrices from cultured cells: subfractionation of nuclei in situ. J Cell Biol 1984;98: 1886-1894.
19. Schimenti KJ, Jacobberger JW. Fixation of mammalian cells for flow cytometric evaluation of DNA content and nuclear immunofluorescence. Cytometry 1992;3:48-59.
20. Lehman JM, Laffin J, Jacobberger JW, Fogleman D. Analysis of simian virus 40 infection of CV-1 cells by quantitative two- color fluorescence with flow cytometry. Cytometry 1988;9:52-59.
21. Jacobberger JW. Flow cytometric analysis of intracellular protein epitopes. In: Stewart C, Nicholson JS, editors, Immunophenotyping. New York: John Wiley & Sons, Inc.; in press.
22. Bauer KD, Jacobberger JW. Analysis of intracellular proteins. Methods Cell Biol 1994;41:351-376.
23. Darzynkiewicz Z, Gong J, Juan G, Ardelt B, Traganos F. Cytometry of cyclin proteins. Cytometry 1996;25:1-13.
24. Marchetti D, Van NT, Gametchu B, Thompson EB, Kobayashi Y, Watanabe F, Barlogie B. Flow cytometric analysis of glucocorticoid receptor using monoclonal antibody and fluoresceinated ligand probes [published erratum appears in Cancer Res 1989 Jun 1;49(11): 3142]. Cancer Res 1989;49:863-869.
25. Brotherick I, Lennard TW, Cook S, Johnstone R, Angus B, Winthereik MP, Shenton BK. Use of the biotinylated antibody DAKO-ER 1D5 to measure oestrogen receptor on cytokeratin positive cells obtained from primary breast cancer cells. Cytometry 1995;20:74-80.
26. Jacobberger JW, Sizemore N, Gorodeski G, Rorke EA. Transforming growth factor beta regulation of epidermal growth factor receptor in ectocervical epithelial cells. Exp Cell Res 1995;220:390-396.
27. Stacchini A, Fubini L, Aglietta M. Flow cytometric detection and quantitative analysis of the GM-CSF receptor in human granulocytes and comparison with the radioligand binding assay. Cytometry 1996; 24:374-381.
28. Poncelet P, Carayon P. Cytofluorometric quantification of cell-surface antigens by indirect immunofluorescence using monoclonal antibodies. J Immunol Methods 1985;85:65-74.
29. Watson JV, Walport MJ. Molecular calibration in flow cytometry with sub-attogram detection limit. J Immunol Methods 1986;93:171-175.
30. Dux R, Kindler-Rohrborn A, Lennartz K, Rajewsky MF. Calibration of fluorescence intensities to quantify antibody binding surface determinants of cell subpopulations by flow cytometry. Cytometry 1991; 12:422-428.
31. Duperray C, Klein B, Durie BG, Zhang X, Jourdan M, Poncelet P, Favier F, Vincent C, Brochier J, Lenoir G, et al. Phenotypic analysis of human myeloma cell lines. Blood 1989;73:566-572.
32. Gratama JW, D'Hautcourt JL, Mandy F, Rothe G, Barnett D, Janossy G, Papa S, Schmitz G, Lenkei R. Flow cytometric quantitation of immunofluorescence intensity: problems and perspectives. European Working Group on Clinical Cell Analysis. Cytometry 1998;33:166-178.
33. Lenkei R, Andersson B. Determination of the antibody binding capacity of lymphocyte membrane antigens by flow cytometry in 58 blood donors. J Immunol Methods 1995;183:267-277.
34. Hess RD, Kuther M, Haessler C. Paetzold S, Braun DG, Brandner,G. Quantitative cytofluorimetric determination of cell membrane-associated large tumor antigen on SV40-transformed cells. Cytometry 1995; 20:81-85.
35. Dawson CD, Scheppler JA, Nicholson JK, Holman RC. Enumeration of antigen sites on cells by flow cytometry. Pathobiology 1991;59:57-61.
36. Filippini G, Griffin S, Uhr M, Eppenberger H, Bonilla J, Cavalli F, Soldati G. A novel flow cytometric method for the quantification of p53 gene expression. Cytometry 1998;31:180-186.
37. Davis KA, Abrams B, Iyer SB, Hoffman RA, Bishop JE. Determination of CD4 antigen density on cells: role of antibody valency, avidity, clones, and conjugation. Cytometry 1998;33:197-205.
38. Lenkei R, Gratama JW, Rothe G, Schmitz G, D'Hautcoun JL, Arekrans A, Mandy F, Marti G. Performance of calibration standards for antigen quantitation with flow cytometry. Cytometry 1998;33:188-196.
39. Iyer SB, Hultin LE, Zawadzki JA, Davis KA, Giorgi JV. Quantitation of CD38 expression using QuanliBRITE beads. Cytometry 1998;33:206-212.
40. Purvis N, Stelzer G. Multi-platform, multi-site instrumentation and reagent standardization. Cytometry 1998;33:156-165.
41. Zenger VE, Vogt R, Mandy F, Schwartz A, Marti GE. Quantitative flow cytomctry: inter-laboratory variation. Cytometry 1998;33:138-145.
42. Graessmann A, Graessmann M, Mueller C. Regulation of SV40 gene expression. Adv Cancer Res 1981;35:111-149.