An aminoacylation-dependent nuclear tRNA export pathway in yeast

Genes and Development

Volumn 14, Issue 7, 2000, Pages 830-840

  Grosshans, Helge ^a   Hurt, Ed ^a   Simos, George ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Aminoacylation; eEF1A; LOS1; Nuclear export; tRNA

Indexed keywords

TRANSFER RNA;

AMINOACYLATION; ARTICLE; CELL NUCLEUS MEMBRANE; FLUORESCENCE IN SITU HYBRIDIZATION; INTRACELLULAR TRANSPORT; NONHUMAN; PRIORITY JOURNAL; RNA TRANSLATION; YEAST;

CARRIER PROTEINS; CELL NUCLEUS; FUNGAL PROTEINS; GENOMIC LIBRARY; HUMANS; INTRONS; MUTAGENESIS; NUCLEAR PORE COMPLEX PROTEINS; NUCLEAR PROTEINS; NUCLEIC ACID CONFORMATION; NUCLEOCYTOPLASMIC TRANSPORT PROTEINS; OLIGONUCLEOTIDE PROBES; RNA, TRANSFER; RNA, TRANSFER, AMINO ACYL; RNA, TRANSFER, ILE; RNA, TRANSFER, LEU; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 0034175551     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (52)

1. Adam, S.A. 1999. Transport pathways of macromolecules between the nucleus and the cytoplasm. Curr. Opin. Cell Biol. 11: 402-406.
2. Aebi, M., G. Kirchner, J.Y. Chen, U. Vijayraghavan, A. Jacobson, N.C. Martin, and J. Abelson. 1990. Isolation of a temperature-sensitive mutant with an altered tRNA nucleotidyltransferase and cloning of the gene encoding tRNA nucleotidyltransferase in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 265: 16216-16220.
3. Amberg, D.C., A.L. Goldstein, and C.N. Cole. 1992. Isolation and characterization of RAT1: An essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA. Genes & Dev. 6: 1173-1189.
4. Arts, G.J., M. Fornerod, and I.W. Mattaj. 1998a. Identification of a nuclear export receptor for tRNA. Curr. Biol. 8: 305-314.
5. Arts, G.J., S. Kuersten, P. Romby, B. Ehresmann, and I.W. Mattaj. 1998b. The role of exportin-t in selective nuclear export of mature tRNAs. EMBO J. 17: 7430-7441.
6. Barbarese, E., D.E. Koppel, M.P. Deutscher, C.L. Smith, K. Ainger, F. Morgan, and J.H. Carson. 1995. Protein translation components are colocalized in granules in oligodendrocytes. J. Cell Sci. 108: 2781-2790.
7. Bertrand, E., F. Houser-Scott, A. Kendall, R.H. Singer, and D.R. Engelke. 1998. Nucleolar localization of early tRNA processing. Genes & Dev. 12: 2463-2468.
8. Billaut-Mulot, O., R. Fernandez-Gomez, M. Loyens, and A. Ouaissi. 1996. Trypanosoma cruzi elongation factor 1-alpha: Nuclear localization in parasites undergoing apoptosis. Gene 174: 19-26.
9. Chamberlain, J.R., Y. Lee, W.S. Lane, and D.R. Engelke. 1998. Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP. Genes & Dev. 12: 1678-1690.
10. Clare, J.J. and S.G. Oliver. 1982. The regulation of RNA synthesis in yeast. V. tRNA charging studies. Mol. Gen. Genet. 188: 96-102.
11. Condeelis, J. 1995. Elongation factor 1 alpha, translation and the cytoskeleton. Trends Biochem. Sci. 20: 169-170.
12. Corbett, A.H., D.M. Koepp, G. Schlenstedt, M.S. Lee, A.K. Hopper, and P.A. Silver. 1995. Rna1p, a Ran/TC4 GTPase activating protein, is required for nuclear import. J. Cell Biol. 130: 1017-1026.
13. Cottrelle, P., M. Cool, P. Thuriaux, V.L. Price, D. Thiele, J.M. Buhler, and P. Fromageot. 1985. Either one of the two yeast EF-1 alpha genes is required for cell viability. Curr. Genet. 9: 693-697.
14. Dinman, J.D. and T.G. Kinzy. 1997. Translational misreading: Mutations in translation elongation factor 1alpha differentially affect programmed ribosomal frameshifting and drug sensitivity. RNA 3: 870-881.
15. Donahue, T.F., A.M. Cigan, E.K. Pabich, and B.C. Valavicius. 1988. Mutations at a Zn(II) finger motif in the yeast eIF-2 beta gene alter ribosomal start-site selection during the scanning process. Cell 54: 621-632.
16. Dorris, D.R., F.L. Erickson, and E.M. Hannig. 1995. Mutations in GCD11, the structural gene for eIF-2 gamma in yeast, alter translational regulation of GCN4 and the selection of the start site for protein synthesis. EMBO J. 14: 2239-2249.
17. Dreher, T.W., O.C. Uhlenbeck, and K.S. Browning. 1999. Quantitative assessment of EF-1alpha.GTP binding to aminoacyl-tRNAs, aminoacyl-viral RNA, and tRNA shows close correspondence to the RNA binding properties of EF-Tu. J. Biol. Chem. 274: 666-672.
18. Gangwani, L., M. Mikrut, Z. Galcheva-Gargova, and R.J. Davis. 1998. Interaction of ZPR1 with translation elongation factor-1alpha in proliferating cells. J. Cell Biol. 143: 1471-1484.
19. Grosshans, H., G. Simos, and E. Hurt. 2000. Transport of tRNA out of the nucleus-direct channeling to the ribosome? J. Struct. Biol. (in press).
20. Harashima, S. and A.G. Hinnebusch. 1986. Multiple GCD genes required for repression of GCN4, a transcriptional activator of amino acid biosynthetic genes in Saccharomyces cerevisiae. Mol. Cell. Biol. 6: 3990-3998.
21. Hellmuth, K., D.M. Lau, F.R. Bischoff, M. Künzler, E.C. Hurt, and G. Simos. 1998. Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA. Mol. Cell. Biol. 18: 6374-6386.
22. Hurt, D.J., S.S. Wang, L. Yu-Huei, and A.K. Hopper. 1987. Cloning and characterization of LOS1, a Saccharomyces cerevisiae gene that affects tRNA splicing. Mol. Cell. Biol. 7: 1208-1216.
23. Izaurralde, E., U. Kutay, C. von Kobbe, I.W. Mattaj, and D. Görlich. 1997. The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. EMBO J. 16: 6535-6547.
24. Kutay, U., G. Lipowsky, E. Izaurralde, F.R. Bischoff, P. Schwarzmaier, E. Hartmann, and D. Görlich. 1998. Identification of a tRNA-specific nuclear export receptor. Mol. Cell 1: 359-369.
25. Lalo, D., C. Carles, A. Sentenac, and P. Thuriaux. 1993. Interactions between three common subunits of yeast RNA polymerases I and III. Proc. Natl. Acad. Sci. 90: 5524-5528.
26. Lipowsky, G., F.R. Bischoff, E. Izaurralde, U. Kutay, S. Schäfer, H.J. Gross, H. Beier, and D. Görlich. 1999. Coordination of tRNA nuclear export with processing of tRNA. RNA 5: 539-549.
27. Lund, E. and J.E. Dahlberg. 1998. Proofreading and aminoacylation of tRNAs before export from the nucleus. Science 282: 2082-2085.
28. Negrutskii, B.S. and A.V. El'skaya. 1998. Eukaryotic translation elongation factor 1 alpha: Structure, expression, functions, and possible role in aminoacyl-tRNA channeling. Prog. Nucleic Acid Res. Mol. Biol. 60: 47-78.
29. Negrutskii, B.S., T.V. Budkevich, V.F. Shalak, G.V. Turkovskaya, and A.V. El'skaya. 1996. Rabbit translation elongation factor 1α stimulates the activity of homologous aminoacyl-tRNA synthetase. FEBS Lett. 382: 18-20.
30. Negrutskii, B.S., V.F. Shalak, P. Kerjan, A.V. El'skaya, and M. Mirande. 1999. Functional interaction of mammalian valyl-tRNA synthetase with elongation factor EF-1α in the complex with EF-1H. J. Biol. Chem. 274: 4545-4550
31. Popenko, V.I., J.L. Ivanova, N.E. Cherny, V.V. Filonenk, S.F. Beresten, A.D. Wolfson, and L.L. Kisselev. 1994. Compartmentalization of certain components of the protein synthesis apparatus in mammalian cells. Eur. J. Cell Biol. 65: 60-69.
32. Reed, V.S., M.E. Wastney, and D.C.H. Yang. 1994. Mechanisms of the transfer of aminoacyl-tRNA from aminoacyl-tRNA synthetase to the elongation factor 1 alpha. J. Biol. Chem. 269: 32932-32936.
33. Sandbaken, M.G. and M.R. Culbertson. 1988. Mutations in elongation factor EF-1 alpha affect the frequency of frame-shifting and amino acid misincorporation in Saccharomyces cerevisiae. Genetics 120: 923-934.
34. Sanders, J., M. Brandsma, G.M. Janssen, J. Dijk, and W. Moller. 1996. Immunofluorescence studies of human fibroblasts demonstrate the presence of the complex of elongation factor-1 beta gamma delta in the endoplasmic reticulum. J. Cell Sci. 109: 1113-1117.
35. Sarkar, S. and A.K. Hopper. 1998. tRNA nuclear export in Saccharomyces cerevisiae: In situ hybridization analysis. Mol. Biol. Cell 9: 3041-3055.
36. Sarkar, S., A.K. Azad, and A.K. Hopper. 1999. Nuclear tRNA aminoacylation and its role in nuclear export of endogenous tRNAs in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. 96: 14366-14371.
37. Schimmel, P. and C.C. Wang. 1999. Getting tRNA synthetases into the nucleus. Trends Biochem. Sci. 24: 127-128.
38. + RNA and nuclear pores. EMBO J. 16: 3256-3271.
39. Senger, B., G. Simos, F.R. Bischoff, A.V. Podtelejnikov, M. Mann, and E.C. Hurt. 1998. Mtr10p functions as a nuclear import receptor for the mRNA binding protein Np13p. EMBO J. 17: 2196-2207.
40. Sharma, K., E. Fabre, H. Tekotte, E.C. Hurt, and D. Tollervey. 1996. Yeast nucleoporin mutants are defective in pre-tRNA splicing. Mol. Cell. Biol. 16: 294-301.
41. Simos, G. 1999. Nuclear export of tRNA. Protoplasma 209: 173-180.
42. Simos, G. and E. Hurt. 1999. Transfer RNA biogenesis: A visa to leave the nucleus. Curr. Biol. 9: R238-R241.
43. Simos, G., A. Segref, F. Fasiolo, K. Hellmuth, A. Shevchenko, M. Mann, and E.C. Hurt. 1996a. The yeast protein Arc1p binds to tRNA and functions as a cofactor for methionyl- and glutamyl-tRNA synthetases. EMBO J. 15: 5437-5448.
44. Simos, G., H. Tekotte, H. Grosjean, A. Segref, K. Sharma, D. Tollervey, and E.C. Hurt. 1996b. Nuclear pore proteins are involved in the biogenesis of functional tRNA. EMBO J. 15: 2270-2284.
45. Simos, G., A. Sauer, F. Fasiolo, and E.C. Hurt. 1998. A conserved domain within Arc1p delivers tRNA to aminoacyl-tRNA synthetases. Mol. Cell 1: 235-242.
46. Song, J.M., S. Picologlou, C.M. Grant, M. Firoozan, M.F. Tuite, and S. Liebman. 1989. Elongation factor EF-1 alpha gene dosage alters translational fidelity in Saccharomyces cerevisiae. Mol. Cell Biol. 9: 4571-4575.
47. Stade, K., C.S. Ford, C. Guthrie, and K. Weis. 1997. Exportin 1 (Crm1p) is an essential nuclear export factor. Cell 90: 1041-1050.
48. Stapulionis, R. and M.P. Deutscher. 1995. A channeled tRNA cycle during mammalian protein synthesis. Proc. Natl. Acad. Sci. 92: 7158-7161.
49. te Heesen, S., R. Knauer, L. Lehle, and M. Aebi. 1993. Yeast Wbp1p and Swp1p form a protein complex essential for oligosaccharyl transferase activity. EMBO J. 12: 279-284.
50. Met by point mutagenesis. Cell 43: 415-422.
51. Wolin, S.L. and A.G. Matera. 1999. The trials and travels of tRNA. Genes & Dev. 13: 1-10.
52. Ziegelbauer, K., P. Babczinski, and W. Schönfeld. 1998. Molecular mode of action of the antifungal beta-amino acid BAY 10-8888. Antimicrob. Agents Chemother. 42: 2197-2205.