Nuclear pore proteins are involved in the biogenesis of functional tRNA

EMBO Journal

Volumn 15, Issue 9, 1996, Pages 2270-2284

  Simos, George ^a   Tekotte, Hildegard ^b   Grosjean, Henri ^c   Segref, Alexandra ^a   Sharma, Kishore ^b   Tollervey, David ^b   Hurt, Eduard C ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c LABORATOIRE D ENZYMOLOGIE ET BIOCHIMIE STRUCTURALES   (France)

Author keywords

Nuclear pore complex; Nucleocytoplasmic transport; Nucleoporin; Pseudouridine synthase; tRNA

Indexed keywords

NUCLEOPORIN; PSEUDOURIDINE; RNA PRECURSOR; SYNTHETASE; TRANSFER RNA;

ARTICLE; CONTROLLED STUDY; HEAT SENSITIVITY; INTRON; NONHUMAN; PRIORITY JOURNAL; RNA SYNTHESIS; XENOPUS; YEAST;

EID: 0029892431     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.1460-2075.1996.tb00580.x     Document Type: Article

References (71)

1. Agostini Carbone, M.L., Solinas, M., Sora, S. and Panzen, L (1991) A gene tightly linked to CEN6 is important for growth of Saccharomyces cerevisiae. Curr. Genet., 19, 1-8.
2. Baim, S.B., Pietras, D.F., Eustice, D.C. and Sherman, F. (1985) A mutation allowing an mRNA secondary structure diminishes translation of Saccharomyces cerevisiae iso-1-cytochrome c. Mol. Cell. Biol., 5, 1839-1846.
3. Becker, J., Melchior, F., Gerke, V., Bischoff, F.R., Ponstingl, H and Wittinghofer, A. (1995) RNA1 encodes a GTPase-activating protein specific for Gsp1p, the Ran/TC4 homologue of Saccharomyces cerevisiae. J. Biol. Chem., 270, 11860-11865.
4. Bischoff, F.R., Krebber, H., Kempf, T., Hermes, I. and Ponstingl, H (1995) Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport. Proc. Natl Acad. Sci. USA, 92, 1749-1753.
5. Björk, G.R. (1995) Biosynthesis and function of modified nucleosides. In Söll, D. and RajBhandary, U.L. (eds), tRNA: Structure, Biosynthesis, and Function. ASM Press, Washington, DC, pp. 165-205.
6. Cheng, Y., Dahlberg, J.E. and Lund, E. (1995) Diverse effects of the guanine nucleotide exchange factor RCC1 on RNA transport. Science, 267, 1807-1810.
7. Clark, M.W. and Abelson, J. (1987) The subnuclear localization of tRNA ligase in yeast. J. Cell Biol., 105, 1515-1526
8. Corbett, A.H., Koepp, D.M., Schlenstedt, G., Lee, M.S., Hopper, A.K. and Silver, P.A. (1995) Rna1p, a Ran/TC4 GTPase activating protein, is required for nuclear import. J. Cell Biol., 130, 1017-1026.
9. Culbertson, M.R. and Winey, M. (1989) Split tRNA genes and their products: a paradigm for the study of cell function and evolution. Yeast, 5, 405-427.
10. Davis, L.I. (1995) The nuclear pore complex. Annu. Rev. Biochem., 64, 865-896.
11. Doye, V. and Hurt, E.C. (1995) Genetic approaches to nuclear pore structure and function. Trends Genet., 11, 193-199.
12. + RNA transport and nuclear pore distribution. EMBO J., 13, 6062-6075.
13. Elledge, S.J. and Davis, R.W (1988) A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene, 70, 303-312.
14. Fabre, E., Boelens, W.C , Wimmer, C., Mattaj, I.W. and Hurt, E.C. (1994) Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif Cell, 78, 275-289.
15. Fischer, U., Huber, J., Boelens, W.C., Mattaj, I.W. and Lührmann, R (1995) The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell, 82, 475-483.
16. Gerace, L (1995) Nuclear export signals and the fast track to the cytoplasm Cell, 82, 341-344.
17. Gorsch, L.C., Dockendorff, T.C. and Cole, C.N. (1995) A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J. Cell Biol., 129, 939-955.
18. Grandi, P., Doye, V. and Hurt, E.C. (1993) Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO J., 12, 3061-3071.
19. Grandi, P., Emig, S., Weise, C., Hucho, F., Pohl, T. and Hurt, E.C. (1995a) A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p. J. Cell Biol., 130, 1263-1273.
20. Grandi, P., Schlaich, N., Tekotte, H. and Hurt, E.C. (1995b) Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p. EMBO J., 14, 76-87.
21. Grosjean, H., Sprinzl, M. and Steinberg, S (1995) Posttranscriptionally modified nucleosides in transfer RNA, their locations and frequencies. Biochimie, 77, 139-141.
22. Grosjean, H., Edqvist, J., Staby, K.B. and Giege, R. (1996) Enzymatic formation of modified nucleosides in tRNA: dependence on tRNA architecture. J. Mol. Biol., 255, 67-85.
23. Haselbeck, R.C. and Greer, C.L. (1993) Minimum intron requirements for tRNA splicing and nuclear transport in Xenopus oocytes. Biochemistry, 32, 8575-8581.
24. Hopper, A.K. (1990) Genetic methods for the study of transacting genes involved in processing of precursors to yeast cytoplasmic tRNAs. Methods Enzymol., 181, 400-421.
25. Hopper, A.K. and Martin, N.C. (1992) Processing of yeast cytoplasmic and mitochondrial precursor tRNAs. In Jones, E.W., Pringle, J.R. and Broach, J.R. (eds), The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, Vol. II, pp. 99-141.
26. Hopper, A.K., Banks, F. and Evangelidis, V. (1978) A yeast mutant which accumulates precursor tRNAs. Cell, 14, 211-219.
27. Hopper, A.K., Schultz, L.D. and Shapiro, R.A. (1980) Processing of intervening sequences: a new yeast mutant which fails to excise intervening sequences from precursor tRNAs. Cell, 19, 741-751.
28. Hopper, A.K., Traglia, H.M and Dunst, R.W. (1990) The yeast RNA1 gene product necessary for RNA processing is located in the cytosol and apparently excluded from the nucleus. J. Cell Biol., 111, 309-321.
29. Hurt, D.J., Wang, S.S., Yu-Huei, L and Hopper, A.K. (1987) Cloning and characterization of LOS1, a Saccharomyces cerevisiae gene that affects tRNA splicing. Mol. Cell. Biol., 7, 1208-1216.
30. Hurt, E.C., McDowall, A. and Schimmang, T. (1988) Nucleolar and nuclear envelope proteins of the yeast Saccharomyces cerevisiae. Eur. J. Cell Biol., 46, 554-563.
31. Izaurralde, E. and Mattaj, I.W. (1995) RNA export. Cell, 81, 153-159.
32. β domains is required for ribosomal RNA processing. EMBO J., 12, 2549-2558.
33. Jarmolowski, A., Boelens, W.C., Izaurralde, E. and Mattaj, I.W. (1994) Nuclear export of different classes of RNA is mediated by specific factors. J. Cell Biol., 124, 627-635.
34. Tyr gene intron is essential for modification of its tRNA product. Nature, 302, 681-687.
35. Kammen, H.O., Marvel, C.C., Hardy, L. and Penhoet, E.E (1988) Purification, structure, properties of Escherichia coli tRNA pseudouridine synthetase I. J Biol. Chem., 263, 2255-2263.
36. Kianz, J.E. and Holm, C. (1990) Cloning by function, an alternative approach for identifying yeast homologs of genes from other organisms. Proc Natl Acad. Sci. USA, 87, 6629-6633.
37. + RNA and constitutive clustering of nuclear pore complexes. Mol. Biol. Cell, 6, 401-417.
38. Lim, F., Morris, C.P., Occhiodoro, F. and Wallace, J.C (1988) Sequence and domain structure of yeast pyruvate carboxylase. J. Biol. Chem., 263, 11493-11497.
39. Maniatis, T , Fritsch, E.T. and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
40. Marck, C., Lefebvre, O., Carles, C., Riva, M., Chaussivert, N., Ruet, A. and Sentenac, A. (1993) The TFIIIB-assembling subunit of yeast transcription factor TFIIIC has both tetratricopeptide repeats and basic helix-loop-helix motifs. Proc. Natl Acad. Sci. USA, 90, 4027-4031.
41. Melchior, F. and Gerace, L. (1995) Mechanisms of nuclear protein import. Curr. Opin. Cell Biol., 7, 310-318.
42. Nehrbass, U., Kern, H., Mutvei, A., Horstmann, H., Marshallsay, B. and Hurt, E.C. (1990) NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain. Cell, 61, 979-989.
43. Nehrbass, U., Fabre, E., Dihlmann, S., Herth, W. and Hurt, E.C. (1993) Analysis of nucleo-cytoplasmic transport in a thermosensitive mutant of the nuclear pore protein NSP1. Eur. J. Cell Biol., 62, 1-12.
44. Nishikura, K. and De Robertis, E.M. (1981) RNA processing in microinjected Xenopus oocytes. Sequential addition of base modifications in a spliced transfer RNA. J. Mol. Biol., 145, 405-420.
45. Nurse, K., Wrzesinski, J., Bakin, A., Lane, B.G. and Ofengand, J. (1995) Purification, cloning, and properties of the tRNA Ψ55 synthase from Escherichia coli. RNA, 1, 102-112.
46. O'Connor, J.P. and Peebles, C.L. (1992) PTA1, an essential gene of Saccharomyces cerevisiae affecting pre-tRNA processing. Mol. Cell. Biol., 12, 3843-3856.
47. Peebles, C.L., Ogden, R.C., Knapp, G. and Abelson, J. (1979) Splicing of yeast tRNA precursors: a two stage reaction. Cell, 18, 27-35.
48. Peebles, C.L., Gegenheimer, P. and Abelson, J. (1983) Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease. Cell, 32, 525-536.
49. 2-dimethylguanosine-specific tRNA methyltransferase. Biochimie, 77, 45-53.
50. Rothstein, R. (1983) One step gene disruption in yeast. Methods Enzymol., 101, 202-211.
51. Samuelsson, T. and Olsson, M. (1990) Transfer RNA pseudouridine synthases in Saccharomyces cerevisiae. J Biol. Chem., 265, 8782-8787.
52. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA-sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA, 74, 5466-5467
53. Schlaich, N.L. and Hurt, E.C. (1995) Analysis of nucleocytoplasmic transport and nuclear envelope structure in yeast disrupted for the gene encoding the nuclear pore protein Nup1p. Eur. J. Cell Biol., 67, 8-14.
54. Sharma, K., Fabre, E , Tekotte, H., Hurt, E.C. and Tollervey, D. (1996) Yeast nucleoporin mutants are defective in pre-tRNA splicing. Mol. Cell. Biol., 16, 294-301.
55. Shen, W.-C., Selvakumar, D., Stanford, D.R. and Hopper, A.K. (1993) The Saccharomyces cerevisiae LOS1 gene involved in pre-tRNA splicing encodes a nuclear protein that behaves as a component of the nuclear matrix. J. Biol. Chem., 268, 19436-19444.
56. Sherman, F. (1990) Guide to yeast genetics and molecular biology. Getting started with yeast. Methods Enzymol., 194, 3-20.
57. Sherman, F., Fink, G.R and Hicks, J.B.N. (1986) Methods in Yeast Genetics: A Laboratory Course Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
58. Simos, G. and Hurt, E.C (1995) Nucleocytoplasmic transport, factors and mechanisms. FEBS Lett., 369, 107-112.
59. Smiossoglou, S., Wimmer, C., Rieger, M., Doye, V., Tekotte, H., Weise, C., Emig, S , Segref, A. and Hurt, E.C. (1996) A novel complex of nucleoporins which includes Sec13p and a Sec13p homologue is essential for normal nuclear pores. Cell, 84, 265-275.
60. Steinberg, S., Misch, A. and Sprinzl, M. (1993) Compilation of tRNA sequences and sequences of tRNA genes. Nucleic Acids Res., 21, 3011-3015.
61. Strobel, M.C. and Abelson, J (1986) Intron mutations affecting splicing of Saccharomyces cerevisiae SUP53 precursor tRNA in vivo and in vitro. Mol. Cell. Biol., 6, 2674-2683.
62. Ile. EMBO J., 13, 4636-4644.
63. met by point mutagenesis. Cell, 43, 415-422.
64. Tranguch, A.J. and Engelke, D.R. (1993) Comparative structural analysis of nuclear RNase P RNAS from yeast. J. Biol. Chem., 268, 14045-14053.
65. Wen, W., Meinkoth, J.L., Tsien, R Y and Taylor, S.S. (1995) Identification of a signal for rapid export of proteins from the nucleus Cell, 82, 463-473.
66. Westaway, S.K. and Abelson, J. (1995) Splicing of tRNA precursors. In Söll, D. and RajBhandary, U.L. (eds), tRNA: Structure, Biosynthesis, and Function. ASM Press, Washington, DC, pp. 79-92.
67. Wimmer, C., Doye, V., Grandi, P., Nehrbass, U. and Hurt, E. (1992) A new subclass of nucleoporins that functionally interacts with nuclear pore protein NSP1. EMBO J., 11, 5051-5061.
68. Wimmer, C., Doye, V., Nehrbass, U., Schlaich, N. and Hurt, E.C. (1993) Approaches towards a genetic analysis of the nuclear pore complex in yeast. In Brown, A.J.P., Tuite, M.F. and McCarthy, J.E.G. (eds), Protein Synthesis and Targeting in Yeast. Springer-Verlag, Berlin, Vol. 71, pp. 269-281.
69. Wrzesinski, J., Bakin, A., Nurse, K., Lane, B.G. and Ofengand, J. (1995a) Purification, cloning, and properties of the 16S RNA pseudouridine 516 synthase from Escherichia coli. Biochemistry, 34, 8904-8913.
70. Phe. RNA, 1, 437-448.
71. Zasloff, M. (1983) tRNA transport from the nucleus in eukaryotic cell: carrier-mediated process. Proc. Natl Acad. Sci. USA, 80, 6436-6440.