Induced sputum cell counts: Their usefulness in clinical practice

European Respiratory Journal

Volumn 16, Issue 1, 2000, Pages 150-158

  Jayaram, L ^a   Parameswaran, K ^a   Sears, M R ^a   Hargreave, F E ^a  

^a ST JOSEPH S HOSPITAL   (Canada)

Author keywords

Airway inflammation; Asthma; Clinical application; Eosinophilic bronchitis; Induced sputum; Sputum cell counts

Indexed keywords

HEMOSIDERIN;

ARTICLE; ASTHMA; CLINICAL PRACTICE; CONTROLLED STUDY; DIAGNOSTIC VALUE; DIFFERENTIAL DIAGNOSIS; EOSINOPHILIA; HUMAN; HUMAN CELL; PRIORITY JOURNAL; SPUTUM ANALYSIS; VALIDATION PROCESS;

CELL COUNT; EOSINOPHILS; GASTROESOPHAGEAL REFLUX; HUMANS; LYMPHOCYTES; MACROPHAGES; NEUTROPHILS; RESPIRATORY TRACT DISEASES; SPUTUM;

EID: 0033939983     PISSN: 09031936     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-3003.2000.16a27.x     Document Type: Article

References (81)

1. Gollasch H. Zur kemitnis des asthmatischen sputums. Fortschritte der Medizin (Berlin) 1889; 7: 361-365.
2. Hargreave FE, Pizzichini E, Pizzichini MMM. Assessment of airway inflammation. In: Barnes P, Grunstein M, Leff A, Woolcock A, eds. Asthma. Vol. 2. Philadelphia, PA, USA, , Lippincott-Raven, 1997; pp. 1433-1450.
3. Djukanovic P, Roche WR, Wilson JW, et al. Mucosal inflammation in asthma: state of the art. Am Rev Respir Dis 1990; 142: 434-457.
4. 2-agonist, terbutaline, on airway inflammation in newly diagnosed asthma: a randomized, double-blind, parallel-group controlled trial. J Allergy Clin Immunol 1992; 90: 32-42.
5. Bousquet J, Vignola AM, Chanez P, Campbell AM, Bonsingore G, Michel FB. Airways remodelling in asthma: no doubt, no more? Int Arch Allergy Immunol 1995; 107: 211-214.
6. Jeffery PK. Bronchial biopsies and airway inflammation. Eur Respir J 1996; 9: 1583-1587.
7. Busse W, Elias J, Sheppard D, Banks-Schlegel S. NHLBI workshop summary. Airway remodeling and repair. Am J Respir Crit Care Med 1999; 160: 1035-1042.
8. Boulet L-P, Becker A, Dérubé D, Beveridge R, Ernst P on behalf of the Canadian Asthma Consensus Group. Canadian Asthma Consensus Report, 1999. CMAJ 1999; 161: Suppl. 11: S1-S12.
9. Pauwels R. Outcome measures in asthma. Eur Respir Rev 1996; 6: 38-39.
10. Haley K, Drazen J. Inflammation and airway function in asthma. What you see is not necessarily what you get. Am J Respir Crit Care Med 1998; 157: 1-3.
11. Pin I, Gibson PG, Kolendowicz R, et al. Use of induced sputum cell counts to investigate airway inflammation in asthma. Thorax 1992; 47: 25-29.
12. Fahy JV, Liu J, Wong H, Boushey HA. Cellular and biochemical analysis of induced sputum from asthmatic and healthy individuals. Am Rev Respir Dis 1993; 146: 1126-1131.
13. Pizzichini E, Pizzichini MMM, Efthimiadis A, et al. Indices of airway inflammation in induced sputum: reproducibility and validity of cell and fluid phase measurements. Am J Respir Crit Care Med 1996; 154: 308-317.
14. Fahy JV, Wong H, Liu J, Boushey HA. Comparison of samples collected by sputum induction and bronchoscopy from asthmatic and healthy subjects. Am J Respir Crit Care Med 1995; 152: 53-58.
15. Maestrelli P, Seatta M, Di Stefano A, et al. Comparison of leukocyte counts in sputum, bronchial biopsies, and bronchoalveolar lavage. Am J Respir Crit Care Med 1995; 152: 1926-1931.
16. Grootendorst DC, Sont JK, Willems LNA, et al. Comparison of inflammatory cell counts in asthma: induced sputum versus bronchoalveolar lavage and bronchial biopsy. Clin Exp Allergy 1997; 27: 769-779.
17. Keatings VM, Evans DJ, O'Connor BJ, Barnes PJ. Cellular profiles in asthmatic airways: a comparison of induced sputum, bronchial washings, and bronchoalveolar lavage fluid. Thorax 1997; 52: 372-374.
18. Pizzichini E, Pizzichini MMM, Kidney JC, et al. Induced sputum, bronchoalveolar lavage and blood from mild asthmatics: inflammatory cells, lymphocyte subsets and soluble markers compared. Eur Respir J 1998; 11: 828-834.
19. Pizzichini E, Pizzichini MMM, Efthimiadis A, Dolovich J, Hargeave FE. Measuring airway inflammation in asthma: eosinophils and cosinophilic cationic protein in induced sputum compared with peripheral blood. J Allergy Clin Immunol 1997; 99: 539-544.
20. Al-Ali MK, Howarth PH. Nitric oxide and the respiratory system in health and disease. Respir Med 1998; 92: 701-715.
21. Pizzichini MM, Pizzichini E, Clelland L, et al. Prednisone dependent asthma: inflammatory indices in induced sputum. Eur Respir J 1999; 13: 15-21.
22. Belda J, Kamada D, Lemiere C, Efthimiadis A, Hargreave FE. The kinetics of clinical and inflammatory indices during an exacerbation of asthma induced by inhaled steroid reduction. Am J Respir Crit Care Med 1999; 159: A123.
23. Pizzichini E, Clelland LJ, Efthimiadis A, Mahony J, Dolovich J, Hargreave FE. Sputum in severe exacerbations of asthma: kinetics of inflammatory indices after prednisone treatment. Am J Respir Crit Care Med 1997; 155: 1501-1508.
24. Parameswaran K, Pizziechini E, Pizzichini MM, Hussack P, Efthimiadis A, Hargreave FE. Clinical judgement of airway inflammation versus sputum cell counts in patients with asthma. Eur Respir J 2000; 15: 486-490.
25. Pavord ID, Pizzichini MMM, Pizzichini E, Hargreave FH. The use of induced sputum to investigate airway inflammation. Thorax 1997; 52: 498-501.
26. Kips JC, Fahy JV, Hargreave FE, Ind PW, In't Veen JCCM. Methods for sputum induction and analysis of induced sputum: a method for assessing airway inflammation in asthma. Eur Respir J 1998; 11 : Suppl. 26, 9s-12s.
27. Wong HH, Faby W. Safety of one method of sputum induction in asthmatic subjects. Am Respir Crit Care Med 1997; 155: 299-303.
28. de la Fuente PT, Romagnoli M, Godard P, Bousquet J, Chanez P. Safety of inducing sputum in patients with asthma of varying severity. Am J Respir Crit Care Med 1998; 157: 1127-1130.
29. Vlachos-Mayer H, Leigh K, Sharon RF, Hussack P, Hargreave FE. Sputum induction for inflammatory indices: safety and success. Am J Respir Crit Care Med 1998; 159: A850.
30. Gershman NH, Wong HH, Liu JT, Mahlmeister MJ, Fahy W. Comparison of two methods of collecting induced sputum in asthmatic subjects. Eur Respir J 1996; 9: 2448-2453.
31. Efthimiadis A, Pizzichinin E, Pizzichini MMM, Hargreave FE. Sputum examination for indices of airway inflammation: laboratory procedures. Astra Draco AB, Lund, Sweden, 1997.
32. In't Veen JCCM, de Gouw HWFM, Smits HH, et al. Repeatability of cellular and soluble markers of inflammation in induced sputum from patients with asthma. Eur Respir J 1996; 9: 2441-2447.
33. Belda J, Leigh R, Parameswaran K, O'Byrne PM, Sears M, Hargreave FE. Induced sputum cell counts in healthy adults. Am J Respir Crit Care Med 2000; 161: 475-478.
34. Gauvreau GM, Doctor J, Watson RM, Jordana M, O'Byrne PM. Effects of inhaled budesonide on allergen-induced airway responses and airway inflammation. Am J Respir Crit Care Med 1996; 154: 1267-1271.
35. Gauvreau GM, Watson GM, O'Byrne PM. Kinetics of allergen-induced airway eosinophilic cytokine production and airway inflammation. Am J Respir Crit Care Med 1999; 160: 640-647.
36. Maestrelli P, Calcagni PG, Saetta MP, et al. Sputum eosinophilia after asthmatic responses induced by isocyanates in sensitized subjects. Clin Exp Allergy 1994; 24: 29-34.
37. Obata H, Dittrick M, Chan H, Chan-Yeung M. Sputum eosinophils and exhaled nitric oxide during late asthmatic reaction in patients with western cedar asthma. Eur Respir J 1999; 160: 93-99.
38. Gibson PG, Wong BJ, Hepperle MJ, et al. A research method to induce and examine a mild exacerbation of asthma by withdrawal of inhaled corticosteroid. Clin Exp Allergy 1992; 22: 525-532.
39. In't Veen JC, Smits HH, Hiernstra PS, Zwinderman AE, Sterk PJ, Bel EH. Lung function and sputum characteristics of patients with severe asthma during an induced exacerbation by double-blind steroid withdrawal. Am J Respir Crit Care Med 1999; 160: 93-99.
40. Lemiere C, Pizzichini MMM, Balkissoon R, et al. Diagnosing occupational asthma: use of induced sputum. Eur Respir J 1999; 113: 484-428.
41. Gibson PG, Dolovich J, Denburg J, Ramsdale EH, Hargreave FE. Chronic cough, eosinophilic, bronchitis without asthma. Lancet 1989; 1: 1346-1348.
42. Gibson PG, Hargreave FE, Girgis-Garbardo A, Morris M, Denburg JA, Dolovich J. Chronic cough with eosinophilic bronchitis: examination for variable airflow obstruction and response to corticosteroid. Clin Exp Allergy 1995; 25: 127-132.
43. Carney IK, Gibson PG, Murree-Allen K, Saltas N, Olson LG, Hensley MJ. A systematic evaluation of mechanisms in chronic cough. Am J Respir Crit Care Med 1997; 156: 211-216.
44. Cai Y, Carty K, Henry RL, Gibson PG. Persistence of sputum eosinophilia in children with controlled asthma when compared with healthy children. Eur Respir J 1998; 311: 848-853.
45. Lemiere C, Efthimiadis A, Hargreave FE. Occupational eosinophilic bronchitis without asthma: an unknown occupational airway disease. J Allergy Clin Immunol 1997; 100: 852-853.
46. Wong AG, Pavord I, Sears MR, Hargreave FE. A case for serial examination of sputum inflammatory cells. Eur Respir J 1996; 9: 2174-2175.
47. Hargreave FE, Leigh R. Induced sputum, eosinophilic bronchitis and COPD. Am J Respir Crit Care Med 1999; 160: 553-557.
48. Keatings VM, Barnes PJ. Granulocyte activation markers in induced sputum: comparison between chronic obstructive pulmonary disease, asthma, and normal subjects. Am J Respir Crit Care Med 1997; 155: 449-453.
49. 1 in smokers are associated with increased levels of sputum neutrophils. Thorax 1996; 51: 267-271.
50. Fahy JV, Wong M, Lu JT, Boushey RA. Analysis of induced sputum after air and ozone exposures in healthy subjects. Environ Res 1995; 70: 77-83.
51. Vagaggini B, Carvnevali S, Macchioni P, et al. Airway inflammatory response to ozone in subjects with different asthma severity. Eur Respir J 1999; 13: 274-280.
52. Holz O, Jorres RA, Timm, P, et al. Am Respir Crit Care Med 1999; 159: 776-784.
53. Nightingale JA, Rogers DF, Hart LA, Kharitonov SA, Chung K, Barnes PJ. Effect of inhaled endotoxin on induced sputum in normal, atopic, and nonatopic asthmatic subjects. Thorax 1998; 53: 563-571.
54. Pizzichini MM, Pizzichini E, Efthimiadis A, et al. Asthma and natural colds: inflammatory indices in induced sputum. A feasibility study. Am J Respir Crit Care Med 1998; 158: 1178-1184.
55. Fahy JV, Kim KW, Liu J, Boushey HA. Prominent neutrophilic inflammation in sputum from subjects with asthma exacerbation. J Allergy Clin Immunol 1995; 95: 843-852.
56. Leigh R, Hargreave FE. Occupational neutrophilic asthma. Can Respir J 1999; 6: 205-208.
57. Hargreave FE. Induced sputum and response to glucocorticoids. J Allergy Clin Immunol 1998; 102: S102-S105.
58. Claman DM, Boushey HA, Liu J, Wong H, Fahy W. Analysis of induced sputum to examine the effects of prednisone on airway inflammation in asthmatic subjects. J Allergy Clin Immunol 1994; 93: 861-869.
59. Fahy JV, Boushey HA. Effect of low-dose beclomethasone dipropionate on asthma control and airway inflammation. Eur Respir J 1998; 11: 1240-1247.
60. Turner MO, Johnston PR, Pizzichim E, Pizzichini MMM, Hussack PA, Hargreave FE. Antiinflammatory effects of salmeterol compared with beelomethasone in eosinophilic mild exacerbations of asthma: a randomized, placebo controlled trial. Can Respir J 1998; 261-268.
61. Jatakanon A, Kharitonov S, Lim S, Barnes PJ. Effect of differing doses of inhaled budesonide on markers of airway inflammation in patients with mild asthma. Thorax 1999; 54: 108-114.
62. Brown RM. Treatment of chronic asthma with prednisone: significance of eosinophils in sputum. Lancet 1958; ii: 1245-1247.
63. Pavord ID, Brightling CE, Woltmann G, Wardlaw AJ. Non-eosinophilic corticosteroid unresponsive asthma. Lancet 1999; 353: 2213-2214.
64. Pizzichini MMM, Pizzichini E, Parameswaran K, et al. Non asthmatic chronic cough: no effect of treatment with an inhaled corticosteroid in patients without sputum eosinophilia. Can Respir J 1999; 6: 323-330.
65. Parameswaran K, Leigh R, Hargreave FE. Sputum eosinophil count to assess compliance with corticosteroid therapy in asthma. J Allergy Clin Immunol 1999; 104: 502-503.
66. Keatings VM, Jatakanon A, Worsdell YM, Barnes PJ. Effects of inhaled and oral glucocorticoids on inflammatory indices in asthma and COPD. Am J Respir Crit Care Med 1997; 155: 541-548.
67. Pizzichini E, Pizzichini MMM, Gibson P, et al. Sputum eosinophilia predicts benefit from prednisone in smokers with chronic obstructive bronchitis. Am J Respir Crit Care Med 1998; 158: 1-7.
68. McIvor RA, Pizzichini E, Turner MO, Hussack PA, Hargreave FE, Sears MR. Potential masking effects of salmeterol on airway inflammation. Am J Respir Crit Care 1998; 157: 924-930.
69. Gibson PG, Simpson JL, Wilson AJ, Saltos N. Use of induced sputum to select add-on therapy in persistent asthma. Eur Respir J 1999; 14: 27s.
70. Calhoun WJ, Lavins BJ, Glass M. Effect of Accolate (zafirlukast) on broncholaveolar lavage fluid (BAL) after segmental antigen bronchoprovocation in patients with mild to moderate asthma. Am J Respir Crit Care Med 1995; 151: A42.
71. Laitinen LA, Laitinen A, Lindqvist A, et al. Effect of zafirlukast (Accolate) on leukotriene E4 (LTE4) induced airway inflammation in asthma. Am J Respir Crit Care Med 1999; 159: A642.
72. Pizzichini E, Leff JA, Reiss TF, et al. Montelukast reduces airway eosinophilic inflammation in asthma: a randomised, controlled trial. Eur Respir J 1999; 14: 12-18.
73. Irwin RS, French CL, Curley FJ, Zawacki JK, Bennett FM. Chronic cough due to gastroesophageal reflux. Clinical, diagnostic, and pathogenic aspects. Chest 1993; 104: 1511-1517.
74. Corwin RW, Irwin RS. The lipid-laden alveolar macrophage as a marker of aspiration in parenchymal lung disease. Am Rev Respir Dis 1985; 132: 576-581.
75. Parameswaran K, Anvari M, Efthimiadis A, Kamada D, Hargreave FE, Allen CJ. Lipid-laden macrophages in induced sputum are a marker of gastric oropharyngeal reflux and possible aspiration. Eur Respir J 2000; (in press).
76. Kelly MM, Leigh R, McKenzie P, Kamada D, Ramsdale EH, Hargreave FE. Induced sputum examination: diagnosis of pulmonary involvement in Fabry disease. Thorax 2000; (in press).
77. Leigh R, Sharon Pt, Efthimiadis A, Karnada D, Hargreave FE, Kitching AD. Diagnosing left ventricular dysfunction from induced sputum examination. Lancet 1999; 354: 833-834.
78. D'Ippolito B, Foresi A, Chetta A, et al. Induced sputum in patients with newly diagnosed sarcoidosis: comparison with bronchial wash and BAL. Chest 1999; 115: 1611-1615.
79. Khajotia M, Mohn A, Pokieser L, Schalleschak J, Vetter N. Induced sputum and cytological diagnosis of lung cancer. Lancet 1991; 338: 966-967.
80. Parameswaran K, Pizzichini MMM, Li D, Pizzichini E, Jeffery PK, Hargreave FE. Serial sputum cell counts in the management of chronic airflow limitation. Eur Respir J 1998; 110: 1405-1408.
81. Jayaram L, Pizzichini MMM, Pizzichini E, et al. Sputum in steroid resistant asthma: a comparison with prednisone dependent asthma. Am J Respir Crit Care Med 1998; 159: A703.