Indices of airway inflammation in induced sputum: Reproducibility and validity of cell and fluid-phase measurements

American Journal of Respiratory and Critical Care Medicine

Volumn 154, Issue 2, 1996, Pages 308-317

  Pizzichini, Emilio ^b   Pizzichini, Marcia M M ^b   Efthimiadis, Ann ^b   Evans, Susan ^b   Morris, Marilyn M ^b   Squillace, Diane ^b   Gleich, Gerald J ^b   Dolovich, Jerry ^b   Hargreave, Frederick E ^a  

^a ST JOSEPH S HOSPITAL   (Canada)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

CELL MARKER; DITHIOTHREITOL; EOSINOPHIL CATIONIC PROTEIN; INTERLEUKIN 5; MAJOR BASIC PROTEIN; METHACHOLINE; NEUROTOXIN; TRYPTASE;

ADULT; ARTICLE; ASTHMA; BRONCHITIS; CELL VIABILITY; CIGARETTE SMOKING; CLINICAL ARTICLE; CLINICAL FEATURE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; FIBRINOGEN BLOOD LEVEL; HUMAN; PRIORITY JOURNAL; QUESTIONNAIRE; RELIABILITY; REPRODUCIBILITY; SKIN TEST; SPIROMETRY; SPUTUM ANALYSIS;

EID: 0029759171     PISSN: 1073449X     EISSN: None     Source Type: Journal    
DOI: 10.1164/ajrccm.154.2.8756799     Document Type: Article

References (39)

1. Pin, I., P. G. Gibson, R. Kolendowicz, A. Girgis-Gabardo, J. Denburg, F. E. Hargreave, and J. Dolovich. 1992. Use of induced sputum cell counts to investigate airway inflammation in asthma. Thorax 47:25-29.
2. Gibson, P. G., A. Girgis-Gabardo, M. M. Morris, S. Mattoli, J. M. Kay, J. Dolovich, J. Denburg, and F. E. Hargreave. 1989. Cellular characteristics of sputum from patients with asthma and chronic bronchitis. Thorax 44:693-699.
3. Pin, I., A. P. Freitag, P. M. O'Byrne, A. Girgis-Gabardo, R. M. Watson, J. Dolovich, J. A. Denburg, and F. E. Hargreave. 1992. Changes in cellular profile of induced sputum after allergen-induced asthmatic response. Am. Rev. Respir. Dis. 145:1265-1269.
4. Fahy, J. V., J. Liu, H. Wong, and H. A. Boushey. 1994. Analysis of cellular and biochemical constituents in induced sputum after allergen challenge: a method for studying allergic airway inflammation. J. Allergy Clin. Immunol. 93:1031-1039.
5. Gibson, P. G., F. E. Hargreave, A. Girgis-Gabardo, M. Morris, J. A. Denburg, and J. Dolovich. 1995. Chronic cough with eosinophilic bronchitis: examination for variable airflow obstruction and response to corticosteroid. Clin. Exp. Allergy 25:127-132.
6. Maestrelli, P., P. G. Calcagni, M. Saetta, A. Di Stefano, J. J. Hosselet, A. Santoanastaso, L. M. Fabbri, and C. E. Mapp. 1994. Sputum eosinophilia after responses induced by isocyanates in sensitized subjects. Clin. Exp. Allergy 24:29-34.
7. Claman, D. M., H. A. Boushey, J. Liu, H. Wong, and J. V. Fahy. 1994. Analysis of induced sputum to examine the effects of prednisone on airway inflammation in asthmatic subjects. J. Allergy Clin. Immunol. 94:861-869.
8. Fahy, J. V., J. Liu, H. Wong, and H. A. Boushey. 1993. Cellular and biochemical analysis of induced sputum from asthmatic and healthy individuals. Am. Rev. Respir. Dis. 147:1126-1131.
9. Popov, T., R. Gottschalk, R. Kolendowicz, J. Dolovich, J. Powers, and F. E. Hargreave. 1994. The evaluation of a cell dispersion method of sputum examination. Clin. Exp. Allergy 24:778-783.
10. Kidney, J. C., A. G. Wong, A. Efthimiadis, M. M. Morris, M. R. Sears, J. Dolovich, and F. E. Hargreave. 1994. Sputum lymphocyte subclasses and activation measured by flow cytometry (abstract). Am. J. Respir. Crit. Care Med. 149:A572.
11. Pizzichini, E., M. M. M. Pizzichini, A. Efthimiadis, F. E. Hargreave, and J. Dolovich. 1996. Measurement of inflammatory indices in induced sputum: effects of selection of the sputum to minimize salivary contamination. Eur. Respir. J. (In press)
12. Killian, K. J., E. Summers, R. M. Watson, P. M. O'Byrne, N. L. Jones, and E. J. M. Campbell. 1993. Factors contributing to dyspnoea during bronchoprovocation and exercise in asthmatics subjects. Eur. Respir. J. 6:1004-1010.
13. American Thoracic Society (statement). 1987. Standardization of spirometry - 1987. Update. Am. Rev. Respir. Dis. 136:1285-1298.
14. Crapo, R. O., A. H. Morris, and R. M. Gardner. 1981. Reference spirometric values using techniques and equipment that meets ATS recommendations. Am. Rev. Respir. Dis. 123:659-694.
15. Juniper, E. F., D. W. Cockcroft, and F. E. Hargreave. 1994. Histamine and Methacholine Inhalation Tests: A Laboratory Tidal Breathing Protocol. Astra Draco AB, Lund, Sweden.
16. Pepys, J. 1975. Skin tests in diagnosis. In P. G. H. Gell, R. R. D. Coombs, and P. J. Lachman, editors. Clinical Aspects of Immunology, 3rd ed. Blackwell Scientific Publications, Oxford. 55-80.
17. Wagner, J. M., K. Bartemes, K. K. Vernof, S. Dunnette, K. P. Offord, J. L. Checkel, and G. J. Gleich. 1993. Analysis of pregnancy associated major basic protein levels throughout gestation. Placenta 14:671-681.
18. Abu-Ghazeleh, R. I., S. L. Dunnette, D. A. Loegering, J. L. Checkel, H. Kita, L. L. Thomas, and G. J. Gleich. 1992. Eosinophil granule proteins in peripheral blood granulocytes. J. Leuk. Biol. 52:611-618.
19. Norman, G. R. 1995. Reliability. In D. L. Streiner and G. R. Norman, editors. Health Measurement Scales: A Practical Guide to Their Development and Use, 2nd ed. Oxford University Press, Oxford, UK. 104-127.
20. Bland, J. M., and D. G. Altman. 1986. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet i:307-310.
21. Cleland, W. W. 1964. Dithiothreitol, a new protective reagent for SH groups. Biochemistry 3:480-482.
22. Wooten, O. J., and M. J. Dulfano. 1978. Improved homogenization techniques for sputum cytology counts. Ann. Allergy 41:150-154.
23. Virchow, J. C., Jr., U. Holscher, and C. Virchow, Sr. 1992. Sputum ECP levels correlate with parameters of airflow obstruction. Am. Rev. Respir. Dis. 146:604-606.
24. Frigas, E., D. A. Loegering, G. O. Solley, G. M. Farrow, and G. J. Gleich. 1981. Elevated levels of eosinophil granule major basic protein in the sputum of patients with bronchial asthma. Mayo Clin. Proc. 56:345-353.
25. Dor, P. J., S. J. Ackerman, and G. J. Gleich. 1984. Charcot-Leyden crystal protein and eosinophil granule major basic protein in sputum of patients with respiratory disease. Am. Rev. Respir. Dis. 130:1072-1077.
26. Fahy, J. V., K. W. Kim, J. Liu, and H. A. Boushey. 1995. Prominent neutrophilic inflammation in sputum from subjects with asthma exacerbation. J. Allergy Clin. Immunol. 95:843-851.
27. Popov, T. A., M. M. M. Pizzichini, E. Pizzichini, R. Kolendovicz, Z. Punthakee, J. Dolovich, and F. E. Hargreave. 1995. Some technical factors influencing the induction of sputum for cell analysis. Eur. Respir. J. 8:559-565.
28. Efthimiadis, A., M. M. M. Pizzichini, S. Weston, E. Pizzichini, S. Evans, J. Dolovich, and F. E. Hargreave. 1996. Sputum cellular and fluid phase components: comparison of the effect of dithiothreitol vs. phosphate buffered saline (abstract). J. Allergy Clin. Immunol. 97:A513.
29. Pizzichini, M. M. M., T. Popov, E. Pizzichini, A. Efthimiadis, P. Hussak, S. Evans, J. Dolovich, and F. E. Hargreave. 1996. Spontaneous and induced sputum compared (abstract). Am. J. Respir. Crit. Care Med. (In press)
30. Efthimiadis, A., M. M. M. Pizzichini, E. Pizzichini, S. Weston, R. Kolendovicz, J. Dolovich, and F. E. Hargreave. 1995. The influence of cell viability and squamous cell contamination on the reliability of sputum differential cell counts (abstract). Am. J. Respir. Crit. Care Med. 151:A384.
31. Iredale, M. J., A. R. Wanklyn, I. P. Phillips, T. Krausz, and P. W. Ind. 1994. Non-invasive assessment of bronchial inflammation in asthma: no correlation between eosinophilia of induced sputum and bronchial responsiveness to inhaled hypertonic saline. Clin. Exp. Allergy 24:940-945.
32. Kirby, J. G., F. E. Hargreave, G. J. Gleich, and P. M. O'Byrne. 1987. Bronchoalveolar cell profiles of asthmatics and nonasthmatic subjects. Am. Rev. Respir. Dis. 136:379-383.
33. Djukanovic, R., J. W. Wilson, K. M. Britten, S. J. Wilson, A. F. Walls, W. R. Roche, P. H. Howarth, and S. T. Holgate. 1990. Quantitation of mast cells and eosinophils in the bronchial mucosa of symptomatic atopic asthmatics and healthy control subjects using immunohistochemistry. Am. Rev. Respir. Dis. 142:863-871.
34. Broide, D. H., G. J. Gleich, A. J. Cuomo, D. A. Coburn, E. C. Federman, L. B. Schwartz, and S. I. Wasserman. 1991. Evidence of ongoing mast cell and eosinophil degranulation in symptomatic asthma airway. J. Allergy Clin. Immunol. 88:637-648.
35. Sedgwick, J. B., W. J. Calhoun, G. J. Gleich, H. Kita, J. S. Abrams, L. B. Schwartz, B. Volovitz, M. Ben-Yaakov, and W. W. Busse. 1991. Immediate and late airway response of allergic rhinitis patients to segmental antigen challenge: characterization of eosinophil and mast cell mediators. Am. Rev. Respir. Dis. 144:1274-1281.
36. Schoonbrood, D. F. M., R. Lutter, F. J. M. Habets, C. M. Roos, K. M. Jansen, and T. A. Out. 1994. Analysis of plasma-protein leakage and local secretion in sputum from patients with asthma and chronic obstructive pulmonary disease. Am. J. Respir. Crit. Care Med. 150: 1519-1527.
37. Tomassini, M., A. Tsicopoulos, P. C. Tai, V. Gruart, A. B. Tonnel, L. Prin, A. Capron, and M. Capron. 1991. Release of granule proteins by eosinophils from allergic and nonallergic patients with eosinophilia on immunoglobulin-dependent activation. J. Allergy Clin. Immunol. 88:365-375.
38. Kroegel, C., J.-C. Virchow, Jr., W. Luttmann, C. Walker, and J. A. Warner. 1994. Pulmonary immune cells in health and disease: the eosinophil leucocyte. Eur. Respir. J. 7:519-543.
39. Adachi, T., S. Motojima, A. Hirata, and S. Makino. 1995. Eosinophil viability-enhancing activity in sputum from patients with bronchial asthma. Am. J. Respir. Crit. Care Med. 151:618-623.