Expression of Mycobacterium smegmatis pyrazinamidase in mycobacterium tuberculosis confers hypersensitivity to pyrazinamide and related amides

Journal of Bacteriology

Volumn 182, Issue 19, 2000, Pages 5479-5485

  Boshoff, H I M ^a   Mizrahi, V ^a  

^a SOUTH AFRICAN MEDICAL RESEARCH COUNCIL   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

AMIDASE; AMIDE; BENZAMIDE; NICOTINAMIDASE; NICOTINAMIDE; PYRAZINAMIDASE; PYRAZINAMIDE; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENETICS; CONCENTRATION RESPONSE; ENZYME ACTIVITY; ENZYME LOCALIZATION; ESCHERICHIA COLI; GENE EXPRESSION; GENE OVEREXPRESSION; MINIMUM INHIBITORY CONCENTRATION; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL;

AMIDOHYDROLASES; ANTITUBERCULAR AGENTS; BENZAMIDES; ESCHERICHIA COLI; GENE EXPRESSION; MUTAGENESIS; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; NIACINAMIDE; NICOTINAMIDASE; PYRAZINAMIDE; SUPPORT, NON-U.S. GOV'T;

ESCHERICHIA COLI; MYCOBACTERIUM TUBERCULOSIS;

EID: 0033797547     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.19.5479-5485.2000     Document Type: Article

References (39)

1. Allelic exchange in Mycobacterium tuberculosis with long linear recombination substrates
2. Purification, gene cloning, targeted knockout, overexpression and biochemical characterization of the major pyrazinamidase from Mycobacterium smegmatis
3. The pH tolerance of unclassified mycobacteria
4. pncA Mutations as a major mechanism of pyrazinmide resistance in Mycobacterium tuberculosis: Spread of a monoresistant strain in Quebec, Canada
5. Evaluating current methods for determination of the susceptibility of mycobacteria to pyrazinmide, conventional, radiometric Bactec and two methods of pyrazinamidase testing
6. SOS induction in mycobacteria: Analysis of the DNA-binding activity of a LexA-like repressor and its role in DNA damage induction of the recA gene from Mycobacterium smegmatis
7. Consensus statement. Global burden of tuberculosis: Estimated incidence, prevalence, and mortality by country. WHO Global Surveillance and Monitoring Project
8. Survival of Mycobacterium avium and Mycobacterium tuberculosis in acidified vacuoles of murine macrophages
9. Construction by homologous recombination and phenotypic characterization of a polA mutant of Mycobacterium smegmatis
10. Gene replacement by UV pretreatment
11. Mycobacterium smegmatis has two pyrazinamidase enzymes, PncA and PzaA
12. Does pyrazinoic acid as an active moiety of pyrazinamide have specific activity against Mycobacterium tuberculosis?
13. Pyrazinamide sterilizing activity in vitro against semidormant Mycobacterium tuberculosis bacterial populations
14. Enhanced gene replacement in mycobacteria
15. Gene replacement and expression of foreign DNA in mycobacteria
16. Genetic systems for mycobacteria
17. A proton-translocating ATPase regulates pH of the bacterial cytoplasm
18. Proteins induced in Escherichia coli by benzoic acid
19. Physical and genetic characterization of chromosomal copies of the Streptomyces coelicolor mini-circle
20. Activation of pyrazinamide and nicotinamide in acidic environment in vitro
21. Mycobacteria as immunogens: Development of expression vectors for use in multiple mycobacterial species
22. Production of mutants in amino acid biosynthetic genes of Mycobacterium tuberculosis by homologous recombination
23. Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update
24. Extracellular enzyme activities potentially involved in the pathogenicity of Mycobacterium tuberculosis
25. Mechanisms of pyrazinamide resistance in mycobacteria: Importance of lack of uptake in addition to lack of pyrazinamidase activity
26. Perturbation of anion balance during inhibition of growth of Escherichia coli by weak acids
27. Determination of pyrazinamide MICs for Mycobacterium tuberculosis at different pHs by the radiometric assay
28. The effect of food preservatives on pH homeostasis in Escherichia coli
29. Mutations in pncA, a gene encoding pyrazinamidase/nicotinamidase, cause resistance to the antituberculous drug, pyrazinamide in tubercle bacillus
30. pH-regulated genes and survival at extreme pH
31. Isolation and characterization of high efficiency plasmid transformation mutants of Mycobacterium smegmatis
32. Determination of drug susceptibility of mycobacteria to pyrazinamide in 7H10 agar
33. The pncA gene from naturally pyrazinamide-resistant Mycobacterium avium encodes pyrazinamidase and confers pyrazinamide susceptibility to resistant M. tuberculosis complex organisms
34. Reduced pyrazinamidase activity and the natural resistance of Mycobacterium kansasii to the antituberculosis drug pyrazinamide
35. Purification and characterization of nicotinamide deamidase from yeast
36. Secretion of ATP-utilizing enzymes, nucleoside diphosphate kinase and ATPase, by Mycobacterium bovis BCG: Sequestration of ATP from macrophage P2Z receptors?
37. Role of acid pH and deficient efflux of pyrazinoic acid in unique susceptibility of Mycobacterium tuberculosis to pyrazinamide