Production of mutants in amino acid biosynthesis genes of Mycobacterium tuberculosis by homologous recombination

Microbiology

Volumn 145, Issue 12, 1999, Pages 3497-3503

  Parish, Tanya ^a   Gordhan, Bhavna G ^b   McAdam, Ruth A ^c   Duncan, Ken ^c   Mizrahi, Valerie ^b,d   Stoker, Neil G ^a  

^a LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

^b NATIONAL HEALTH LABORATORY SERVICE   (South Africa)

^c GLAXOSMITHKLINE   (United Kingdom)

^d UNIVERSITY OF THE WITWATERSRAND   (South Africa)

Author keywords

Auxotrophs; Casamino acids; Gene replacement; lacZ; Pre treated DNA

Indexed keywords

BACTERIAL DNA; HYGROMYCIN;

ARTICLE; AUXOTROPHIC MUTANT; BACTERIAL GENE; BACTERIUM MUTANT; CROSSING OVER; DENATURATION; ELECTROPORATION; GENETIC RECOMBINATION; MOLECULAR CLONING; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; ULTRAVIOLET RADIATION;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; PROKARYOTA; UNCULTURED ACTINOMYCETE;

EID: 0033401316     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-145-12-3497     Document Type: Article

References (19)

1. Aldovini, A., Husson, R. N. & Young, R. A. (1993). The uraA locus and homologous recombination in Mycobacterium bovis BCG. J Bacteriol 175, 7282-7289.
2. Balasubramanian, V., Pavelka, M. S., Jr, Bardarov, S. S., Martin, J., Weisbrod, T. R., McAdam, R. A., Bloom, B. R. & Jacobs, W. R., Jr (1996). Allelic exchange in Mycobacterium tuberculosis with long linear recombination substrates. J Bacteriol 178, 273-279.
3. Belisle, J. T. & Sonnenberg, M. G. (1998). Isolation of genomic DNA from mycobacteria. In Mycobacteria Protocols, pp. 31-44. Edited by T. Parish & N. G. Stoker. Totowa, NJ: Humana Press.
4. Cersini, A., Salvia, A. M. & Bernardini, M. L. (1998). Intracellular multiplication and virulence of Shigella flexneri auxotrophic mutants. Infect Immun 66, 549-557.
5. Cole, S. T., Brosch, R., Parkhill, J. & 39 other authors (1998). Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393, 537-544.
6. Guleria, I., Teitelbaum, R., McAdam, R. A., Kalpana, G., Jacobs, W. R., Jr & Bloom, B. R. (1996). Auxotrophic vaccines for tuberculosis. Nature Med 2, 334-337.
7. Hinds, J., Mahenthiralingam, E., Kempsell, K. E., Duncan, K., Stokes, R. W., Parish, T. & Stoker, N. G. (1999). Enhanced gene replacement in mycobacteria. Microbiology 145, 519-527.
8. Humayun, M. Z. (1998). SOS and Mayday: multiple inducible mutagenic pathways in Eschericbia coli. Mol Microbiol 30, 905-910.
9. Kalpana, G. V., Bloom, B. R. & Jacobs, W. R., Jr (1991). Insertional mutagenesis and illegitimate recombination in mycobacteria. Proc Natl Acad Sci USA 88, 5433-5437.
10. Mizrahi, V. & Andersen, S. J. (1998). DNA repair in Mycobacterium tuberculosis: what have we learnt from the genome sequence? Mol Microbiol 29, 1331-1339.
11. Moral, C. H., del Castillo, E. F., Fierro, P. L., Cortes, A. V., Castillo, J. A., Soriano, A. C., Salazar, M. S., Peralta, B. R. & Carrasco, G. N. (1998). Molecular characterization of the Aeromonas hydrophila aroA gene and potential use of an auxotrophic aroA mutant as a live attenuated vaccine. Infect Immun 66, 1813-1821.
12. O'Callaghan, D., Maskell, D., Liew, F. Y., Easmon, C. S. & Dougan, G. (1988). Characterization of aromatic- and purine-dependent Salmonella typhimurium: attention, persistence, and ability to induce protective immunity in BALB/c mice. Infect Immun 56, 419-423.
13. Pavelka, M. S., Jr & Jacobs, W. R., Jr (1999). Comparison of the construction of unmarked deletion mutations in Mycobacterium smegmatis, Mycobacterium bovis Bacillus Calmette-Guerin, and Mycobacterium tuberculosis H37Rv by allelic exchange. J Bacteriol 181, 478-4789.
14. Pelicic, V., Reyrat, J. M. & Gicquel, B. (1996). Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria. J Bacteriol 178, 1197-1199.
15. Pelicic, V., Jackson, M., Reyrat, J. M., Jacobs, W. R., Jr, Gicquel, B. & Guilhot, C. (1997). Efficient allelic exchange and transposon mutagenesis in Mycobacterium tuberculosis. Proc Natl Acad Sci USA 94, 10955-10960.
16. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
17. Santos, A. R., Demiranda, A. B., Lima, L. M., Suffys, P. N. & Degrave, W. M. (1992). Method for high yield preparation in large and small scale of nucleic acids from mycobacteria. J Microbiol Methods 15, 83-94.
18. Valentine, P. J., Devore, B. P. & Heffron, F. (1998). Identification of three highly attenuated Salmonella typhimurium mutants that are more immunogenic and protective in mice than a prototypical aroA mutant. Infect Immun 66, 3378-3383.
19. Wilson, T., Wards, B. J., White, S. J., Skou, B., de Lisle, G. W. & Collins, D. M. (1997). Production of avirulent Mycobacterium bovis strains by illegitimate recombination with deoxyribonucleic acid fragments containing an interrupted ahpC gene. Tuber Lung Dis 78, 229-235.