Quantitation of plasma membrane expression of a fusion protein of Na/H exchanger NHE3 and green fluorescence protein (GFP) in living PS120 fibroblasts

Journal of Histochemistry and Cytochemistry

Volumn 48, Issue 11, 2000, Pages 1479-1491

  Janecki, A J ^a   Janecki, M ^a   Akhter, S ^a   Donowitz, M ^a  

^a UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

Author keywords

Confocal microscopy; Green fluorescent protein; Morphometric analysis; NHE3

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; SODIUM PROTON EXCHANGE PROTEIN 3; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BINDING KINETICS; CELLULAR DISTRIBUTION; COMPUTER GRAPHICS; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; DIAGNOSTIC VALUE; HAMSTER; IMMUNOHISTOCHEMISTRY; MORPHOMETRICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTON SODIUM EXCHANGE; QUANTITATIVE DIAGNOSIS; RABBIT; REPRODUCIBILITY; THREE DIMENSIONAL IMAGING;

ANIMALIA; CRICETINAE; ORYCTOLAGUS CUNICULUS;

EID: 0033761761     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540004801105     Document Type: Article

References (42)

1. C-Terminal domains of Na(+)/H(+) exchanger isoform 3 are involved in the basal and serum-stimulated membrane trafficking of the exchanger
2. Phorbol 12-myristate 13-acetate down-regulates Na,K-ATPase independent of its protein kinase C site: Decrease in basolateral cell surface area
3. Imaging exocytosis and endocytosis
4. Green fluorescent protein as a marker for gene expression
5. FACS-optimized mutants of the green fluorescent protein (GFP)
6. Red-shifted excitation mutants of the green fluorescent protein
7. Short-term regulation of NHE3 by growth factors/protein kinases involves vesicle trafficking in epithelial cells and fibroblasts
8. Molecular studies of members of the mammalian Na/H exchanger gene family
9. The epithelial sodium-hydrogen antiporter Na+/H+ exchanger 3 accumulates and is functional in recycling endosomes
10. Ultrastructural changes in oxyntic cells associated with secretory function: A membrane recycling hypothesis
11. Isolation and properties of fibroblast mutants overexpressing an altered Na+/H+ antiporter
12. Green fluorescent protein: Applications in cell biology
13. Biotinylation and assessment of membrane polarity: Caveats and methodological concerns
14. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer
15. Kinetic analysis of secretory protein traffic and characterization of Golgi to plasma membrane transport intermediates in living cells
16. Basic fibroblast growth factor stimulates surface expression and activity of Na+/H+ exchanger NHE3 via mechanism involving phosphatidylinositol 3-kinase
17. Subcellular redistribution is involved in acute regulation of the brush border Na+/H+ exchanger isoform 3 in human colon adenocarcinoma cell line Caco-2. Protein kinase C-mediated inhibition of the exchanger
18. Constitutive and regulated membrane expression of aquaporin 1 and aquaporin 2 water channels in stably transfected LLC-PK1 epithelial cells
19. Parathyroid hormone action on phosphate transporter mRNA and protein in rat renal proximal tubules
20. Green fluorescent protein as a noninvasive intracellular pH indicator
21. Low temperature-induced transport blocks as tools to manipulate membrane traffic
22. Endosomal recycling of the Na+/H+ exchanger NHE3 isoform is regulated by the phosphatidylinositol 3-kinase pathway
23. Arginine-427 in the Na+/glucose cotransporter (SGLT1) is involved in trafficking to the plasma membrane
24. Endocytosis and molecular sorting
25. Localization, trafficking, and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells
26. Crystal structure of the Aequorea victoria green fluorescent protein
27. Na+/H+ exchanger subtypes in the renal tubule: Function and regulation in physiology and disease
28. Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy
29. A specific mutation abolishing Na+/H+ antiport activity in hamster fibroblasts precludes growth at neutral and acidic pH
30. Trafficking of glut4-green fluorescent protein chimaeras in 3T3-L1 adipocytes suggests distinct internalization mechanisms regulating cell surface glut4 levels
31. Primary structure of the Aequorea victoria green-fluorescent protein
32. Rapid endocytosis of the cystic fibrosis transmembrane conductance regulator chloride channel
33. Solvent perturbations of Aequarea green fluorescent protein
34. pH-dependent fluorescence of a heterologously expressed Aequorea green fluorescent protein mutant: In situ spectral characteristics and applicability to intracellular pH estimation
35. Intracellular pH measurements in Ehrlich ascites tumor cells utilizing spectroscopic probes generated in situ
36. Spectral perturbations of the Aequorea green fluorescent protein
37. The molecular structure of green fluorescent protein
38. Comparison of GLUT4 and GLUT1 subcellular trafficking in basal and insulin-stimulated 3T3-L1 cells
39. Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein
40. Redistribution of Na+/H+ exchanger isoform NHE3 in proximal tubules induced by acute and chronic hypertension