Role of disulfides and sulfhydryl groups in agonist and antagonist binding in serotonin(1A) receptors from bovine hippocampus

Cellular and Molecular Neurobiology

Volumn 20, Issue 6, 2000, Pages 665-681

  Harikumar, K G ^a   John, P Thomas ^a   Chattopadhyay, Amitabha ^a  

^a CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY   (India)

Author keywords

5 HT(1A) receptor; 8 OH DPAT; Bovine hippocampus; DTT; MPPF; NEM

Indexed keywords

DISULFIDE; G PROTEIN COUPLED RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATE; SEROTONIN 1A AGONIST; SEROTONIN 1A ANTAGONIST; SEROTONIN 1A RECEPTOR; THIOL GROUP;

ANIMAL TISSUE; ARTICLE; BINDING SITE; CATTLE; CHEMICAL MODIFICATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DISULFIDE BOND; DRUG RECEPTOR BINDING; GENE LOCATION; HIPPOCAMPUS; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DOMAIN;

8-HYDROXY-2-(DI-N-PROPYLAMINO)TETRALIN; AMINOPYRIDINES; ANIMALS; BINDING SITES; CATTLE; CELL MEMBRANE; DITHIOTHREITOL; ETHYLMALEIMIDE; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); HIPPOCAMPUS; KINETICS; LIGANDS; PIPERAZINES; RADIOLIGAND ASSAY; RECEPTORS, SEROTONIN; RECEPTORS, SEROTONIN, 5-HT1; SEROTONIN AGONISTS; SEROTONIN ANTAGONISTS; TRITIUM;

ANIMALIA; BOS TAURUS; BOVINAE; HIPPOCAMPUS HIPPOCAMPUS;

EID: 0033696443     PISSN: 02724340     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1007046707845     Document Type: Article

References (63)

1. Cloning, functional expression, and mRNA tissue distribution of the rat 5-hydroxytryptamine(1A) receptor gene
2. Acceleration of the effect of selected antidepressant drugs in major depression by 5-HT(1A) antagonists
3. Heterologous expression of the serotonin 5-HT(1A) receptor in neural and nonneural cell lines
4. A role for the serotonin system in the mechanism of action of antidepressant treatments: Preclinical evidence
5. Molecular biology of 5-HT receptors
6. A molecular mechanism for the cleavage of a disulfide bond as the primary function of agonist binding to G-protein-coupled receptors based on theoretical calculations supported by experiments
7. Modeling of the agonist binding site of serotonin human 5-HT(1A), 5-HT(1Dα) and 5-HT(1Dβ) receptors
8. A rapid filtration assay for soluble receptors using polyethylenimine-treated filters
9. A transient, neuron-wide form of CREB-mediated long-term facilitation can be stabilized at specific synapses by local protein synthesis
10. Identification of residues important for ligand binding to the human 5-hydroxytryptamine(1A) serotonin receptor
11. Cloning and differentiation-induced expression of a murine serotonin(1A) receptor in a septal cell line
12. Dependence of critical micelle concentration of a zwitterionic detergent on ionic strength: Implications in receptor solubilization
13. Photophysics of a neurotransmitter: Ionization and spectroscopic properties of serotonin
14. The G-protein nanomachine
15. 2 adrenergic receptor
16. Production of specific anti-rat 5-HT(1A) receptor antibodies in rabbits injected with a synthetic peptide
17. Physical evidence of the coupling of solubilized 5-HT(1A) binding sites with G regulatory proteins
18. 3H]8-hydroxy-2-(di-n-propylamino)tetralin in the rat hippocampus corresponds to an oxidized state of the 5-hydroxytryptamine(1A) receptor
19. The genomic clone G-21 which resembles a β-adrenergic receptor sequence encodes the 5-HT(1A) receptor
20. Identification in the μ-opioid receptor of cysteine residues responsible for inactivation of ligand binding by thiol alkylating and reducing agents
21. 3H-PAT
22. Metal ion and guanine nucleotide modulations of agonist interaction in G-protein-coupled serotonin(1A) receptors from bovine hippocampus
23. Modulation of agonist and antagonist interactions in serotonin(1A) receptors by alcohols
24. Differential discrimination of G-protein coupling of serotonin(1A) receptors from bovine hippocampus by an agonist and an antagonist
25. Effect of alcohols on G-protein coupling of serotonin(1A) receptors from bovine hippocampus
26. Elevated anxiety and antidepressant-like responses in serotonin 5-HT(1A) receptor mutant mice
27. The role of conserved aspartate and serine residues in ligand binding and in function of the 5-HT(1A) receptor: A site directed mutation study
28. Receptor binding studies, a brief outline
29. Structure and function of the brain serotonin system
30. 2 dopamine receptor is exposed in the binding-site crevice
31. G protein-coupled receptors I. Diversity of receptor-ligand interactions
32. Evidence for disulfide bonds in membrane-bound and solubilized opioid receptors
33. Cysteine residues 110 and 187 are essential for the formation of correct structure in bovine rhodopsin
34. 2-adrenoceptors from GTP-binding proteins by N-ethylmaleimide
35. Adrenergic receptors as model for G protein-coupled receptors
36. An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleofide regulatory proteins
37. Agonists and antagonists bind to different domains of the cloned kappa opioid receptor
38. Study of the interaction between aryloxypropanolamines and Asn386 in helix VII of the human 5-hydroxytryptamine(1A) receptor
39. A potential 5-HT(1A) receptor antagonist: p-MPPI
40. 4-(2'-methoxy)-phenyl)-1[2'-(n-2''-pyridinyl)-p-iodobenzamido]ethyl-piperazin e, ([(125)I]p-MPPI) as a new selective radioligand of serotonin-1A sites in rat brain: In vitro binding and autoradiographic studies
41. In vivo binding of [(123)I]4-(2'-methoxy)phenyl)-1-[2'-(N-2''-pyridinyl)-p-iodobenzamido]ethyl-p iper azine, p-MPPI, to 5-HT(1A) receptors in rat brain
42. A serotonin receptor gene (5HT1A) variant found in a Tourette's syndrome patient
43. 2 adrenergic receptor: Effect of DTT on receptor conformation monitored by circular dichroism and fluorescence spectroscopy
44. Chemical modfications of striatal A(2A) adenosine receptors: A possible role for tyrosine at the ligand binding sites
45. 3H]-OH-DPAT binding sites in rat cerebral cortex and hippocampus
46. Blockade of 5-HT(1A) receptors compensates loss of hippocampal cholinergic neurotransmission involved in working memory of rats
47. 2-adrenergic receptor: How structure elucidates function
48. Distribution of serotonin receptors
49. Increased anxiety of mice lacking the serotonin(1A) receptor
50. 5-Hydroxytryptamine receptors
51. Serotonin receptor 1A knockout: An animal model of anxiety-related disorder
52. Increased vulnerability to cocaine in mice lacking the serotonin-1B receptor
53. 7) activating cAMP formation
54. 1 muscarinic acetylcholine receptor: Role of conserved cysteines in receptor function
55. Measurement of protein using bicinchoninic acid
56. The family of G-protein-coupled receptors
57. Structure and function of G protein-coupled receptors
58. Molecular modelling of UH-301 and 5-HT(1A) receptor interactions
59. Effects of novel 5-HT(1A) receptor antagonists on measures of postsynaptic 5-HT(1A) receptor activation in vivo
60. 7 receptors: Current knowledge and future prospects
61. 2 receptor: Identification of amino acids necessary for ligand binding and receptor activation
62. 5-Hydroxytryptamine receptors