Ligand stabilization of the β2 adrenergic receptor: Effect of DTT on receptor conformation monitored by circular dichroism and fluorescence spectroscopy

Biochemistry

Volumn 35, Issue 46, 1996, Pages

  Lin, Sansan ^a   Gether, Ulrik ^a   Kobilka, Brian K ^a,b  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADRENERGIC RECEPTOR BLOCKING AGENT; BETA 2 ADRENERGIC RECEPTOR; DISULFIDE; DITHIOTHREITOL;

ARTICLE; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; FLUORESCENCE SPECTROSCOPY; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE;

AMINO ACID SEQUENCE; CIRCULAR DICHROISM; DITHIOTHREITOL; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, ADRENERGIC, BETA-2; SPECTROMETRY, FLUORESCENCE; SULFHYDRYL REAGENTS;

EID: 0030448201     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi961619+     Document Type: Article

References (25)

1. Chen, Y. H., Yang, J. T., & Chau, K. H. (1974) Biochemistry 13, 3350-3359.
2. Dohlman, H. G., Caron, M. G., DeBlasi, A., Frielle, T., & Lefkowitz, R. J. (1990) Biochemistry 29, 2335-2342.
3. Fraser, C. M. (1989) J. Biol. Chem. 264, 9266-9270.
4. Gether, U., Lin, S., & Kobilka, B. K. (1995) J. Biol. Chem. 270, 28268-28275.
5. Guan, X. M., Kobilka, T. S., & Kobilka, B. K. (1992) J. Biol. Chem. 267, 21995-21998.
6. Hein, L., & Kobilka, B. K. (1995) Neuropharmacology 34, 357-366.
7. Karnik, S. S., & Khorana, H. G. (1990) J. Biol. Chem. 265, 17520-17524.
8. Karnik, S. S., Sakmar, T. P., Chen, H. B., & Khorana, H. G. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 8459-8463.
9. Kobilka, B. K. (1992) Annu. Rev. Neurosci. 15, 87-114.
10. Kobilka, B. K. (1995) Anal. Biochem. 231, 269-271.
11. Kurtenbach, E., Curtis, C. A., Pedder, E. K., Aitken, A., Harris, A. C., & Hulme, E. C. (1990) J. Biol. Chem. 265, 13702-13708.
12. Lakowicz, J. R. (1983) in Principles of Fluorescence Spectroscopy, pp 344-363, Plenum Press, New York.
13. Noda, K., Saad, Y., Graham, R. M., & Karnik, S. S. (1994) J. Biol. Chem. 269, 6743-6752.
14. Park, K., Perczel, A., & Fasman, G. D. (1992) Protein Sci. 1, 1032-1049.
15. Peterson, G. L., Toumadje, A., Johnson, W. J., & Schimerlik, M. I. (1995) J. Biol. Chem. 270, 17808-17814.
16. Savarese, T. M., & Fraser, C. M. (1992) Biochem. J. 1, 1-19.
17. Savarese, T. M., Wang, C. D., & Fraser, C. M. (1992) J. Biol. Chem. 267, 11439-11448.
18. Scholtz, J. M., Qian, H., York, E. J., Stewart, J. M., & Baldwin, R. L. (1991) Biopolymers 31, 1463-1470.
19. Strader, C. D., Candelore, M. R., Hill, W. S., Sigal, I. S., & Dixon, R. A. (1989) J. Biol. Chem. 264, 13572-13578.
20. Strader, C. D., Gaffney, T., Sugg, E. E., Candelore, M. R., Keys, R., Patchett, A. A., & Dixon, R. A. (1991) J. Biol. Chem. 266, 5-8.
21. Strader, C. D., Fong, T. M., Tota, M. R., Underwood, D., & Dixon, R. A. (1994) Annu. Rev. Biochem. 63, 101-132.
22. Suryanarayana, S., & Kobilka, B. K. (1993) Mol. Pharmacol. 44, 111-114.
23. Tota, M. R., & Strader, C. D. (1990) J. Biol. Chem. 265, 16891-16897.
24. Traxler, K. W., & Dewey, T. G. (1994) Biochemistry 33, 1718-1723.
25. Wright, M., & Drummond, G. I. (1983) Biochem. Pharmacol. 32, 509-515.