Glucose-stimulated and self-limiting insulin production by glucose 6-phosphatase promoter driven insulin expression in hepatoma cells

Gene Therapy

Volumn 7, Issue 21, 2000, Pages 1802-1809

  Chen, R ^a   Meseck, M ^a   McEvoy, R C ^a   Woo, S L C ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

Glucose; Glucose 6 phosphatase promoter; Insulin; Regulated insulin gene expression; Type 1 diabetes

Indexed keywords

GLUCOSE; GLUCOSE 6 PHOSPHATASE; INSULIN; VIRUS VECTOR;

ADENOVIRUS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DIET RESTRICTION; EXPRESSION VECTOR; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE TARGETING; GENETIC TRANSDUCTION; GLUCOSE BLOOD LEVEL; HEPATOMA CELL; HORMONAL REGULATION; HUMAN; HUMAN CELL; INSULIN BLOOD LEVEL; INSULIN DEPENDENT DIABETES MELLITUS; INSULIN SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; RAT; TARGET ORGAN; TRANSCRIPTION REGULATION; VIRUS RECOMBINANT;

ADENOVIRIDAE; ANIMALIA;

EID: 0033693585     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301306     Document Type: Article

References (41)

1. The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus
2. Gene therapy for diabetes mellitus in rats by hepatic expression of insulin
3. Hepatic insulin gene expression as treatment for type 1 diabetes mellitus in rats
4. Reversal of diabetes in mice by implantation of human fibroblasts genetically engineered to release mature human insulin
5. Human proinsulin conversion in the regulated and the constitutive pathways of transfected AtT20 cells
6. Processing of mutated proinsulin with tetrabasic cleavage sites to mature insulin reflects the expression of furin in nonendocrine cell lines
7. Genetically engineered proinsulin constitutively processed and secreted as mature, active insulin
8. Synthesis and processing of genetically modified human proinsulin by rat myoblast primary cultures
9. Regulated expression of mature human insulin in the liver of transgenic mice
10. Insulin production by engineered muscle cells
11. Recent advances in hepatic glucose 6-phosphatase regulation and function
12. Effects of hyperglycemia on glucose production and utilization in humans. Measurement with [23H]-, [33H]-, and [614C] glucose
13. Mechanism by which hyperglycemia inhibits hepatic glucose production in conscious rats. Implications for the pathophysiology of fasting hyperglycemia in diabetes
14. A multicompohent insulin response sequence mediates a strong repression of mouse glucose-6-phosphatase gene transcription by insulin
15. Regulation of rat liver glucose-6-phosphatase gene expression in different nutritional and hormonal states: Gene structure and 5'-flanking sequence
16. Glucose regulates in vivo glucose-6-phosphatase gene expression in the liver of diabetic rats
17. Plasma insulin, C-peptide, and proinsulin concentrations in obese ans nonobese individuals with varying degrees of glucose tolerance
18. Transfection of AtT-20ins cells with GLUT-2 but not GLUT-1 confers glucose-stimulated insulin secretion. Relationship to glucose metabolism
19. Novel insulinoma cell lines produced by iterative engineering of GLUT2, glucokinase, and human insulin expression
20. Cellular characterization of pituitary adenoma cell line (AtT20 cell) transfected with insulin, glucose transporter type 2 (GLUT2) and glucokinase genes: Insulin secretion in response to physiological concentrations of glucose
21. Engineering a glucose-responsive human insulin-secreting cell line from islets of langerhans isolated from a patient with persistent hyperinsulinemic hypoglycemia of infancy
22. Regulation of insulin secretion from novel engineered insulinoma cell lines
23. Engineered cell lines for insulin replacement in diabetes: Current status and future prospects
24. Insulin-releasing pituitary cells as a model for somatic cell gene therapy in diabetes mellitus
25. Regulatable production of mature insulin from a hepatocyte cell line: Insulin production is up-regulated by cAMP and glucocorticoids, and down-regulated by insulin
26. Regulatable production of insulin from primary-cultured hepatocytes: Insulin production is up-regulated by glucagon and cAMP and down-regulated by insulin
27. The repression of hormone-activated PEPCK gene expression by glucose is insulin-independent but requires glucose metabolism
28. Glucose responsiveness of a reporter gene transduced into hepatocytic cells using a retroviral vector
29. Transcriptional regulation by glucose in the liver
30. Transcriptional glucose signaling through the glucose response element is mediated by the pentose phosphate pathway
31. Carbon flux via the pentose phosphate pathway regulates the hepatic expression of the glucose-6-phosphatase and phosphoenolpyruvate carboxykinase genes in conscious rats
32. Glucokinase expression in rat hepatoma cells induces glucose uptake and is rate limiting in glucose utilization
33. Stimulation of glucose-6-phosphatase gene expression by glucose and fructose-2,6-bisphosphate
34. Central role for phosphatidylinositide 3-kinase in the repression of glucose-6-phosphatase gene transcription by insulin
35. Hepatocyte nuclear factor-1 acts as an accessory factor to enhance the inhibitory action of insulin on mouse glucose-6-phosphatase gene transcription
36. Cis-regulation of the L-type pyruvate kinase gene promoter by glucose, insulin and cyclic AMP
37. Two CACGTG motifs with proper spacing dictate the carbohydrate regulation of hepatic gene transcription
38. Identification of a glucose response element in the promoter of the rat glucagon receptor gene
39. Regulation of protein secretion through controlled aggregation in the endoplasmic reticulum
40. Methods for construction of adenovirus vectors
41. A simple phase-extraction assay for chloramphenicol acyltransferase activity