Regulatable production of insulin from primary-cultured hepatocytes: Insulin production is up-regulated by glucagon and cAMP and down-regulated by insulin

Gene Therapy

Volumn 5, Issue 7, 1998, Pages 888-895

  Lu, D ^a   Tamemoto, H ^a   Shibata, H ^a   Saito, I ^b   Takeuchi, T ^a  

^a GUNMA UNIVERSITY   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

Gene therapy; Hepatocytes; Insulin; PEPCK; Regulatable expression

Indexed keywords

CYCLIC AMP; DNA; GLUCAGON; INSULIN; ISOBUTYLMETHYLXANTHINE; MESSENGER RNA; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP); PROINSULIN; WORTMANNIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; GENE EXPRESSION REGULATION; INSULIN RELEASE; LIVER CELL CULTURE; MALE; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROMOTER REGION; RAT; RECOMBINANT GENE;

ANIMALIA;

EID: 0031828799     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3300677     Document Type: Article

References (49)

1. Rosenzweig JL. Principles of insulin therapy. In: Kahn CR, Weir, GC (eds). Joslin's Diabetes Mellitus. Lea & Febiger: Philadelphia, 1994, pp 460-488.
2. Newgard CB. Cellular engineering for the treatment of metabolic disorders: prospects for therapy in diabetes. Biotechnology 1992; 10: 1112-1120.
3. Hughes HD, Johnson JH, Quaade C, Newgard CB. Engineering of glucose-stimulated insulin secretion and biosynthesis in nonislet cells. Proc Natl Acad Sci USA 1992; 89: 688-692.
4. ins cells with GLUT-2 but not GLUT-1 confers glucose-stimulated insulin secretion. J Biol Chew 1993; 268: 15205-15212.
5. Newgard CB Cellular engineering and gene therapy strategies for insulin replacement in diabetes. Diabetes 1994; 43: 341-350.
6. Clark SA et al. Novel insulinoma cell lines produced by iterative engineering of GLUT2, glucokinase, and human insulin expression. Diabetes 1997; 46: 958-967.
7. Hohmeier HE et al. Regulation of insulin secretion from novel engineered insulinoma cell lines. Diabetes 1997; 46: 968-977.
8. Lipes MA et al. Insulin-secreting non-islet cells are resistant to autoimmune destruction. Proc Natl Acad Sci USA 1996; 93: 8595-8600.
9. Yanagita M, Nakayama K, Takeuchi T. Processing of mutated promsulin with tetrabasic cleavage sites to bioactive insulin in the nonendocrine cell line, COS-7. FEBS Lett 1992; 311: 55-59.
10. Yanagita M, Hoshino H, Nakayama K, Takeuchi T. Processing of mutated proinsulin with tetrabasic cleavage sites to mature insulin reflects the expression of furin in nonendocrine cell lines. Endocrinology 1993; 133: 639-644.
11. Nishigori T, Yanagita M, Takeuchi T. Proinsulin cleaved by furin is processed to chromatographically mature insulin by carboxypeptidases in non-neuroendocrine cells. Peptides 1996; 17: 789-796.
12. Groskreutz DJ, Sliwkowski MX, Gorman CM. Genetically engineered proinsulin constitutively processed and secreted as mature, active insulin. J Biol Chem 1994; 269: 6241-6245.
13. Simonson GD, Groskreutz DJ, Gorman CM, Macdonald MJSynthesis and processing of genetically modified human proinsulin by rat myoblast primary cultures. Hum Gene Ther 1996; 7: 71-78.
14. Docherty H et al. Engineering insulin secretion in non-neuroendocrine cells for use in the treatment of diabetes mellitus. Exp Clin Endocrinol Diabetes 1997; 105: A50 (Abstr.).
15. Lu D, Hoshino H, Takeuchi T. Regulatable production of mature insulin from a hepatocyte cell line: insulin production is upregulated by cAMP and glucocorticoids, and down-regulated by insulin. FEBS Lett 1996; 399- 37-42.
16. Granner D, Pilkis S. The genes of hepatic glucose metabolism. J Biol Chem 1990; 265: 10173-10176.
17. Clarke SD, Abraham S. Gene expression: nutrient control of preand posttranscriptional events. FASEB J 1992; 6: 3146-3152.
18. Tanaka M et al. Molecular cloning of a group of mouse pancreatic islet β cell-related genes by random cDNA sequencing. Diabetolgia 1995; 38: 381-386.
19. 14 gene. J Biol Chem 1992; 267: 13222-13228.
20. Lm Z, Thompson KS, Towle HC. Carbohydrate regulation of the rat L-type kinase gene requires two nuclear factors: LF-A1 and a member of the c-myc family. J Biol Chem 1993; 268: 12787-12795.
21. Sasaki K et al, Multihormonal regulation of phosphoenolpyruvate carboxy-kinase gene transcription The dominant role of insulin. J Biol Chem 1984; 259: 15242-15251.
22. Petersen DD, Magnuson MA, Granner DK. Location and characterization of two widely separated glucocorticoid response elements in the phosphoenolpyruvate carboxykinase gene. Mol Cell Biol 1988; 8: 96-104.
23. O'Brien RM et al. Identification of a sequence in the PEPCK gene that mediates a negative effect of insulin on transcription. Science 1990; 249: 533-537.
24. O'Brien RM et al. Potential convergence of insulin and cAMP signal transduction systems at the phosphoenolpyruvate carboxykinase (PEPCK) gene promoter through CCAAT/Enhancer binding protein (C/EBP). J Biol Chem 1994; 269: 30419-30428.
25. Sharp DVV et al. Insulin independence after islet transplantation into type I diabetic patient. Diabetes 1990; 39: 515-518.
26. Weir GC, Bonner-Weir S. Scientific and political impediments to successful islet transplantation. Diabetes 1997: 46: 1247-1256.
27. Carroll PB et al. Long-term (>3-year) insulin independence in a patient with pancreatic islet cell transplantation following upper abdominal exenteration and liver replacement for fibrolamellar hepatocellular carcinoma. Transplantation 1995; 59: 875-879.
28. Porksen N et al. Coordinate pulsatile insulin secretion by chronic intraportally transplanted islets in the isolated perfused rat liver. J Clin Invest 1994; 94: 219-227.
29. Ginot F et al. Transfection of hepatic genes into adult rat hepatocytes in primary culture and their tissue-specific expression. Eur J Biochem 1989;180: 289-294.
30. Miller AD, Rosman GJ. Improved retroviral vectors for gene transfer and expression. BioTechniques 1989; 7: 980-990.
31. Rettinger SD et al. Liver-directed gene therapy: quantitative evaluation of promoter elements by using in vivo retroviral transduction. Proc Natl Acad Sci USA 1994; 91: 1460-1464
32. Kaneda Y, Iwai K, Uchida T. Introduction and expression of the human insulin gene in adult rat liver. J Biol Chem 1989; 264: 12126-12129.
33. Cristiano RJ et al Hepatic gene therapy: efficient gene delivery and expression in primary hepatocytes utilizing a conjugated adenovirus - DNA complex. Proc Natl Acad Sci USA 1993; 90: 11548-11552.
34. Tripathy SK, Black HB, Goldwasser E, Leiden JM. Immune responses to transgene-encoded proteins limit the stability of gene expression after injection of replication-defective adenovirus vectors. Nature Med 1996; 2: 545-550.
35. Kay MA et al. Long-term hepatic adenovirus-mediated gene expression in mice following CTLA4Ig administration. Nat Genet 1995; 11: 191-197.
36. Miyake M et al. Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc Natl Acad Sci USA 1996; 93: 1320-1324.
37. Liu G et al. Efficient adenovirus-mediated ectopic gene expression of human lipoprotein lipase in human hepatic (HepG2) cells. Hum Gene Ther 1997; 8: 205-214.
38. Ui M, Okada T, Hazeki K, Hazeki O. Wortmannin as a unique probe for an intracellular signalling protein, phosphoinositide 3-kinase. Trends Biol Sci 1995; 20: 303-307.
39. Kolodka TM, Finegold M, Moss L, Woo SLC. Gene therapy for diabetes mellitus in rats by hepatic expression of insulin. Proc Natl Acad Sci USA 1995; 92: 3293-3297.
40. Simpson AM, Tuch BE, Swan MA, Tu J, Marshall GM. Functional expression of the human insulin gene in a human hepatoma cell line (HEP G2). Gene Therapy 1995; 2: 223-231.
41. Kusunoki M, Cooney GJ, Hara T, Storlien LH. Amelioration of high-fat feeding-induced insulin resistance in skeletal muscle with the antiglucocorticoid RU486. Diabetes 1995; 44: 718-720.
42. Gremlich S, Roduit R, Thorens B. Dexamethasone induces posttranslational degradation of GLUT2 and inhibition of insulin secretion in isolated pancreatic β cells. J Biol Chem 1997; 272: 3216-3222.
43. Unger RH, Orel L. Glucagon and the A cell New Engl J Med 1981; 304: 1575-1580.
44. Precious B, Russel WC. Growth, purification and titration of adenoviruses. In: Mahy BWJ (ed). Virology. A Practical Approach. IRI Press: Oxford, 1985, pp 193-205.
45. Li D, Schuetz EG, Guzelian PS. Hepatocyte culture in study of P450 regulation. In: Waterman MR, Johnson EF (eds). Methods Enzymol. Academic Press: San Diego, 1991, pp 335-344.
46. Hoshino H, Konda Y, Takeuchi T. Co-expression of proproteinprocessing endoprotease furin and its substrate transforming growth factor βl and the differentiation of rat hepatocytes. FEBS Lett 1997; 419: 9-12
47. Mashima H et al. Betacellulin and activin A coordinately convert amylase-secreting pancreatic AR42J cells into insulin-secreting cells J Clin Invest 1996; 97: 1647-1654.
48. Coalson RE. Methods for staining glycogen. In: Clark G (ed). Staining Procedures, 4th edn. Williams & Wilkins- Baltimore, 1981, pp 195-196.
49. Taylor NA, Docherty K. Sequence requirements for processing of proinsulin in transfected mouse pituitary AtT20 cells. Biochem J 1992; 286: 619-622.