Mechanism of RNase T1: Concerted triester-like phosphoryl transfer via a catalytic three-centered hydrogen bond

Chemistry and Biology

Volumn 7, Issue 8, 2000, Pages 651-658

  Loverix, Stefan ^a   Winqvist, Anna ^b   Strömberg, Roger ^c   Steyaert, Jan ^a  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^b STOCKHOLM UNIVERSITY   (Sweden)

^c KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Catalysis; Mechanism; Ribonuclease; Thio effect

Indexed keywords

ENZYME ACTIVE SITE; HYDROGEN BOND; MOLECULAR INTERACTION; PHOSPHOROTHIOIC ACID; RIBONUCLEASE; STEREOSPECIFICITY; TARGETED MUTAGENESIS; WILD RELATIVE;

PHOSPHOTRANSFERASE; PROTON; RIBONUCLEASE T1; RNA;

ARTICLE; BINDING SITE; CATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; CONFORMATION; GENETICS; HYDROGEN BOND; KINETICS; METABOLISM; SITE DIRECTED MUTAGENESIS; STEREOISOMERISM;

BINDING SITES; CATALYSIS; HYDROGEN BONDING; KINETICS; MODELS, MOLECULAR; MOLECULAR CONFORMATION; MUTAGENESIS, SITE-DIRECTED; PHOSPHOTRANSFERASES; PROTONS; RIBONUCLEASE T1; RNA; STEREOISOMERISM;

EID: 0033626550     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(00)00005-3     Document Type: Article

References (44)

1. Limits to Catalysis by Ribonuclease A
2. Reconsidering the energetics of ribonuclease catalysed RNA hydrolysis
3. Stereochemistry of the transesterification step of pancreatic ribonuclease
4. The active site and mechanism of action of bovine pancreatic ribonuclease
5. Value of general acid-base catalysis to ribonuclease A
6. Ribonuclease revisited: Catalysis via the classical general acid-base mechanism or a triester-like mechanism?
7. On the mechanism of action of ribonuclease A: Relevance of enzymatic studies with a p-nitrophenylphosphate ester and a thiophosphate ester
8. Energetics of the catalytic reaction of ribonuclease A: A computational study of alternative mechanisms
9. Structure (1.3 Å) and charge states of a ribonuclease A-uridine vanadate complex: Implications for the phosphate ester hydrolysis mechanism
10. Transphosphorylation catalyzed by ribonuclease A: Computational study using ab initio effective fragment potentials
11. Ribonuclease A
12. Introduction: The ribonuclease A superfamily
13. Molecular structure of a new family of ribonucleases
14. The structural and sequence homology of a family of microbial ribonucleases
15. 1-atomic dissection of the enzyme-substrate interactions
16. 1
17. 1 acts as base catalyst when the true catalytic base, glutamic acid-58, is replaced by alanine
18. 1 with asparagine and glutamine replacements: Analysis of double mutant cycles at one position
19. 1 and 3'GMP suggests geometry of enzymic reaction path. An X-ray study
20. 1 as studied by double and triple mutants
21. Phosphorylations: Transfers of phosphate esters to water or other acceptors
22. Nucleoside phosphorothioates
23. Determination of RNA-protein contacts using thiophosphate substitutions
24. Role of metal ions in the hydrolysis reaction catalyzed by RNase P RNA from Bacillus subtilis
25. 1
26. An engineered ribonuclease preferring phosphorethioate RNA
27. 1: Viscosity effects indicate that the rate-limiting step of GpN transesterification depends on the nature of N
28. Hydrolytic reactions of the diastereomeric phosphoromonothioate analogs of uridylyl(3',5')uddine: Kinetics and mechanisms for desulfurization, phosphoester hydrolysis, and transesterification to the 2',5'-isomers
29. Mechanism of phosphatidylinositol-phospholipase c. 2. reversal of a thio effect by site-directed mutagenesis
30. Nucleophilic activation by positioning in phosphoryl transfer catalyzed by nucleoside diphosphate kinase
31. Impaired transition state complementarity in the hydrolysis of O-arylphosphorothioates by pretoin-tyrosine phosphatases
32. Diastereomers of 5'-O-adenosyl 3'-O-uddyl phosphorethioate: Chemical synthesis and enzymatic properties
33. Double-mutant cycles: A powerful tool for analyzing protein structure and function
34. 1 analyzed by time-resolved crystallography
35. Models for enzyme-catalysed phosphate transfer: Comparisons of reactivity towards a neighbouring hydroxy group for phosphodiester anions and acids. General base catalysis of the cyslisation of a hydroxyalkyl phosphate triester
36. 1
37. 1x2'-guanosine monophosphate at 1.9-Å resolution
38. 1
39. Synthesis of diribonucleoside phosphorothioates via stereospecific sulfurization of H-phosphonate diesters
40. Relationships between apparent binding energies measured in site-directed mutagenesis experiments and energetics of binding and catalysis