A decade of protein engineering on ribonuclease T1 atomic dissection of the enzyme-substrate interactions

European Journal of Biochemistry

Volumn 247, Issue 1, 1997, Pages 1-11

  Steyaert, Jan ^a,b  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^b VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

Author keywords

Catalytic mechanism; Double mutant cycle; Functional cooperativity; Nucleotide binding; Protein engineering; Ribonuclease T1; Subsite interaction; Substrate specificity

Indexed keywords

RIBONUCLEASE;

BINDING SITE; CONFORMATIONAL TRANSITION; ENZYME BINDING; ENZYME CONFORMATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; GENETIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; REVIEW;

EID: 0030877826     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.t01-1-00001.x     Document Type: Review

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