Dissecting histidine interactions of ribonuclease T1 with asparagine and glutamine replacements: Analysis of double mutant cycles at one position

Journal of Molecular Biology

Volumn 275, Issue 4, 1998, Pages 651-661

  De Vos, Stefan ^a   Doumen, Jan ^a   Langhorst, Ulrike ^a   Steyaert, Jan ^a  

^a VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

Author keywords

Double mutant cycles; Protein engineering; Protein stability; Ribonuclease T1

Indexed keywords

ASPARAGINE; GLUTAMINE; HISTIDINE; IMIDAZOLE; NITROGEN; PROTON; RIBONUCLEASE; UREA;

AMINO ACID SUBSTITUTION; ARTICLE; DENATURATION; ENZYME STABILITY; ENZYME STRUCTURE; GENETIC ENGINEERING; HYDROGEN BOND; PH; PRIORITY JOURNAL; PROTEIN CONFORMATION; X RAY CRYSTALLOGRAPHY;

EID: 0032579203     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1997.1480     Document Type: Article

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