An engineered ribonuclease preferring phosphorothioate RNA

Nature Structural Biology

Volumn 5, Issue 5, 1998, Pages 365-368

  Loverix, Stefan ^a   Winquist, Anna ^b   Strömberg, Roger ^c   Steyaert, Jan ^a  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^b STOCKHOLM UNIVERSITY   (Sweden)

^c KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIC GMP; DRUG DERIVATIVE; GUANOSINE 2',3' CYCLOPHOSPHOROTHIOATE; GUANOSINE 2',3'-CYCLOPHOSPHOROTHIOATE; NUCLEOTIDE; RIBONUCLEASE; RNA;

AMINO ACID SUBSTITUTION; CATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME SPECIFICITY; GENETICS; LETTER; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; CATALYSIS; CYCLIC GMP; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; RIBONUCLEASES; RNA; SUBSTRATE SPECIFICITY; THIONUCLEOTIDES;

EID: 0032066341     PISSN: 10728368     EISSN: None     Source Type: Journal    
DOI: 10.1038/nsb0598-365     Document Type: Letter

References (32)

1. Fersht, A. R. Enzyme Structure and Mechanism (W.H. Freeman and Company, New York; 1985).
2. Eckstein, F. Ann. Rev. Biochem. 54, 367-402 (1985).
3. Milligan, J. F. & Uhlenbeck, O. C. Biochemistry 28, 2849-2855 (1989).
4. Dahm, S. C. & Uhlenbeck, O. C. Biochemistry 30, 9464-9469 (1991).
5. Eckstein, F., Schulz, H. H., Ruterjans, H., Haar, W. & Maurer, W. Biochemistry 11, 3507-3512 (1972).
6. Heinemann, U. & Saenger, W. Nature 299, 27-31 (1982).
7. Walz, F. G., Osterman, H. L. & Libertin, C. Arch. Biochem. Biophys. 195, 95-102 (1979).
8. Steyaert, J. Eur. J. Biochem. 247, 1-11 (1997).
9. Heinemann, U. & Hahn, U. In Protein-Nucleic Acid Interactions, 111-141 (MacMillan, London; 1989).
10. Zegers, I. et al. Nature Struct. Biol. 5, 280-283 (1998).
11. Steyaert, J., Hallenga, K., Wyns, L. & Stanssens, P., Biochemistry 29, 9064-9072.
12. Heydenreich, A. et al. Eur. J. Biochem. 218, 1005-1012 (1993).
13. Steyaert, J. & Wyns, L. J. Mol. Biol. 229, 770-781 (1993).
14. Steyaert, J., Haikal, A. F., Wyns, L. & Stanssens, P. Biochemistry 30, 8666-8670.
15. Doumen, J. et al. Protein Sci. 5, 1523-1530 (1996).
16. Steyaert, J., Wyns, L. & Stanssens, P. Biochemistry 30, 8661-8665 (1991).
17. Steyaert, J., Opsomer, C., Wyns, L. & Stanssens, P. Biochemistry 30, 494-499 (1991).
18. Horovitz, A. Folding & Design 1, 121-126 (1996).
19. Lienhard, G. E. Science 180, 149-154 (1973).
20. Herschlag, D., Piccirilli, J. A. & Cech, T. R. Biochemistry 30, 4844-4854 (1991).
21. Fersht, A. R. Biochemistry 27, 1577-1580 (1988).
22. Saenger, W. Principles of Nucleic Acid Structure (Springer-Verlag, New York; 1984).
23. Fersht, A. R. et al. Nature 314, 235-238 (1985).
24. Wilkinson, A. J., Fersht, A. R., Blow, D. M. & Winter, G. Biochemistry 22, 3581-3586 (1983).
25. Gregoret, L. M., Rader, S. D., Fletterick, R. J. & Cohen, F. E. Protein: Struct. Fund. Genet, 9, 99-107 (1991).
26. Herschlag, D. & Jencks, W. P. J. Am. Chem. Soc. 111, 7587-7596 (1989).
27. Hengge, A. C. & Cleland, W. W. J. Am. Chem. Soc. 112, 7421-7422 (1990).
28. Burgers, P. M. J. & Eckstein, F. Biochemistry 18, 592-596 (1979).
29. Aimer, H. & Stromberg, R. Tetrahedron Lett. 294, 3723-3726 (1991).
30. Oivanen, M., Ora, M., Aimer, H., Stromberg, R. & Lonnberg, H. J. Org. Chem. 60, 5620-5627 (1995).
31. Almer, H. & Stromberg, R. J. Am. Chem. Soc. 118, 7921-7928 (1996).
32. Pace, C. N., Vajdos, F., Fee, L., Grimsley, G., & Gray, T. Prot. Sci. 4, 2411-2423 (1995).