Na+-driven flagellar motor resistant to phenamil, an amiloride analog, caused by mutations in putative channel components

Journal of Molecular Biology

Volumn 285, Issue 4, 1999, Pages 1537-1547

  Kojima, Seiji ^a   Asai, Yukako ^a   Atsumi, Tatsuo ^b   Kawagishi, Ikuro ^a   Homma, Michio ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b SUZUKA NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

Author keywords

Amiloride; Flagella; Motor; Phenamil; Sodium channel

Indexed keywords

AMILORIDE DERIVATIVE; MEMBRANE PROTEIN; PHENAMIL; SODIUM CHANNEL; SODIUM ION;

AMINO ACID SUBSTITUTION; ARTICLE; CELL MOTILITY; CONTROLLED STUDY; FLAGELLUM; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL;

EID: 0033613936     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1998.2377     Document Type: Article

References (60)

1. Asai Y., Kojima S., Kato H., Nishioka N., Kawagishi I., Homma M. Putative channel components for the fast-rotating sodium-driven flagellar motor of a marine bacterium. J. Bacteriol. 179:1997;5104-5110.
2. +-driven flagellar motors of alkalophilic Bacillus strains by the amiloride analog phenamil. J. Bacteriol. 172:1990;1634-1639.
3. Atsumi T., Maekawa Y., Yamada T., Kawagishi I., Imae Y., Homma M. Effect of viscosity on swimming by the lateral and polar flagella of Vibrio alginolitycus. J. Bacteriol. 178:1996;5024-5026.
4. Bartolomé B., Jubete Y., Martinez E., Cruz F. D. Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives. Gene. 102:1991;75-78.
5. Blair D. F. How bacteria sense and swim. Annu. Rev. Microbiol. 49:1995;489-522.
6. Blair D. F., Berg H. C. Restoration of torque in defective flagellar motors. Science. 242:1988;1678-1681.
7. Blair D. F., Berg H. C. The MotA protein of E. coli is a proton-conducting component of the flagellar motor. Cell. 60:1990;439-449.
8. Blair D. F., Berg H. C. Mutations in the MotA protein of Escherichia coli reveal domains critical for proton conduction. J. Mol. Biol. 221:1991;1433-1442.
9. Blair D. F., Kim D. Y., Berg H. C. Mutant MotB proteins in Escherichia coli. J. Bacteriol. 173:1991;4049-4055.
10. Block S. M., Berg H. C. Successive incorporation of force-generating units in the bacterial rotary motor. Nature. 309:1984;470-472.
11. Chun S. Y., Parkinson J. S. Bacterial motility: membrane topology of the Escherichia coli MotB protein. Science. 239:1988;276-278.
12. +exchanger gene (NHE1) and amplification of the mutated allele confer amiloride resistance upon chronic acidosis. Proc. Natl Acad. Sci. USA. 90:1993;4508-4512.
13. +exchanger-1. Biochemistry. 36:1997;2951-2959.
14. Cragoe E. J. Jr, Woltersdorf O. W., Bicking J. B., Kwong S. F., Jones J. H. Pyrazine diuretics. II. N-amidino-3-amino- 5-substituted 6 halopyazinecarboxamides. J. Med. Chem. 10:1967;66-75.
15. Cragoe E. J. Jr, Kleyman T. R., Simchowitz L. Amiloride and its Analogs: Unique Cation Transport Inhibitors. 1992;VCH Publishers, Inc. New York.
16. Dean G. D., Macnab R. M., Stader J., Matsumura P., Burks C. Gene sequence and predicted amino acid sequence of the MotA protein, a membrane-associated protein required for flagellar rotation in Escherichia coli. J. Bacteriol. 159:1984;991-999.
17. De Mot R., Vanderleyden J. The C-terminal sequence conservation between OmpA-related outer membrane proteins and MotB suggests a common function in both Gram-positive and Gram-negative bacteria, possibly in the interaction of these domains with peptidoglycan. Mol. Microbiol. 12:1994;333-334.
18. Francis N. R., Sosinsky G. E., Thomas D., DeRosier D. J. Isolation, characterization and structure of bacterial flagellar motors containing the switch complex. J. Mol. Biol. 235:1994;1261-1270.
19. Fuller C. M., Berdiev B. K., Shlyonsky V. G., Ismailov I. I., Benos D. J. Point mutations in αbENaC regulate channel gating, ion selectivity, and sensitivity to amiloride. Biophys. J. 72:1997;1622-1632.
20. Garza A. G., Harris H. L., Stoebner R. A., Manson M. D. Motility protein interactions in the bacterial flagellar motor. Proc. Natl Acad. Sci. USA. 92:1995;1970-1974.
21. Garza A. G., Biran R., Wohlschlegel J. A., Manson M. D. Mutations in motB suppressible by changes in stator or rotor components of the bacterial flagellar motor. J. Mol. Biol. 258:1996a;270-285.
22. Garza A. G., Bronstein P. A., Valdez P. A., Harris H. L., Manson M. D. Extragenic suppression of motA missense mutations of Escherichia coli. J. Bacteriol. 178:1996b;6116-6122.
23. Grant S. G., Jessee J., Bloom F. R., Hanahan D. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl Acad. Sci. USA. 87:1990;4645-4649.
24. Homma M., Oota H., Kojima S., Kawagishi I., Imae Y. Chemotactic responses to an attractant and a repellent by the polar and lateral flagellar systems of Vibrio alginolyticus. Microbiology. 142:1996;2777-2783.
25. +-driven bacterial flagellar motors. J. Bioenerg. Biomembr. 21:1989;705-716.
26. Irikura V. M., Kihara M., Yamaguchi S., Sockett H., Macnab R. M. Salmonella typhimurium fliG and fliN mutations causing defects in assembly, rotation, and switching of the flagellar motor. J. Bacteriol. 175:1993;802-810.
27. +channels determines apparent channel conductance, ion selectivity, and amiloride sensitivity. Biophys. J. 69:1995;1789-1800.
28. +channel. J. Biol. Chem. 272:1997;21075-21083.
29. Kawagishi I., Okunishi I., Homma M., Imae Y. Removal of the periplasmic DNase before electroporation enhances efficiency of transformation in a marine bacteriumVibrio alginolyticus. Microbiology. 140:1994;2355-2361.
30. Kojima S., Atsumi T., Muramoto K., Kudo S., Kawagishi I., Homma M. Vibrio alginolyticus mutants resistant to phenamil, a specific inhibitor of the sodium-driven flagellar motor. J. Mol. Biol. 265:1997;310-318.
31. Kudo S., Magariyama Y., Aizawa S.-I. Abrupt changes in flagellar rotation observed by laser dark-field microscopy. Nature. 346:1990;677-680.
32. +antiporter fromVibrio parahaemolyticus. J. Bacteriol. 179:1997;7600-7602.
33. Lin C. M., Kieberemmons T., Villalobos A. P., Foster M. H., Wahlgren C., Kleyman T. R. Topology of an amiloride-binding protein. J. Biol. Chem. 269:1994;2805-2813.
34. Lloyd S. A., Blair D. F. Charged residues of the rotor protein FliG essential for torque generation in the flagellar motor of Escherichia coli. J. Mol. Biol. 266:1997;733-744.
35. Lloyd S. A., Tang H., Wang X., Billings S., Blair D. F. Torque generation in the flagellar motor of Escherichia coli: evidence of a direct role for FliG but not for FliM or FliN. J. Bacteriol. 178:1996;223-231.
36. Magariyama Y., Sugiyama S., Muramoto K., Maekawa Y., Kawagishi I., Imae Y., Kudo S. Very fast flagellar rotation. Nature. 371:1994;752.
37. McCarter L. L. MotX, the channel component of the sodium-type flagellar motor. J. Bacteriol. 176:1994a;5988-5998.
38. McCarter L. L. MotY, a component of the sodium-type flagellar motor. J. Bacteriol. 176:1994b;4219-4225.
39. Morales B. M., Backman A., Bagdasarian M. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene. 97:1991;39-47.
40. Muramoto K., Sugiyama S., Cragoe E. J. Jr, Imae Y. Successive inactivation of the force-generating units of sodium-driven bacterial flagellar motors by a photoreactive amiloride analog. J. Biol. Chem. 269:1994;3374-3380.
41. Muramoto K., Kawagishi I., Kudo S., Magariyama Y., Imae Y., Homma M. High-speed rotation and speed stability of the sodium-driven flagellar motor in Vibrio alginolyticus. J. Mol. Biol. 251:1995;50-58.
42. Muramoto K., Magariyama Y., Homma M., Kawagishi I., Sugiyama S., Imae Y., Kudo S. Rotational fluctuation of the sodium-driven flagellar motor of Vibrio alginolyticus induced by binding of inhibitors. J. Mol. Biol. 259:1996;687-695.
43. Okunishi I., Kawagishi I., Homma M. Cloning and characterization of motY, a gene coding for a component of the sodium-driven flagellar motor inVibrio alginolyticus. J. Bacteriol. 178:1996;2409-2415.
44. +exchanger that confer sensitivity to pharmacological antagonists. J. Biol. Chem. 271:1996;19922-19927.
45. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Habor Laboratory Press, Plainview, New York.
46. Schild L., Schneeberger E., Gautschi I., Firsov D. Identification of amino acid residues in the α, β, and γ subunits of the epithelial sodium channel (ENaC) involved in amiloride block and ion permeation. J. Gen. Physiol. 109:1997;15-26.
47. Sharp L. L., Zhou J., Blair D. F. Features of MotA proton channel structure revealed by tryptophan-scanning mutagenesis. Proc. Natl Acad. Sci. USA. 92:1995a;7946-7950.
48. Sharp L. L., Zhou J., Blair D. F. Tryptophan-scanning mutagenesis of MotB, an integral membrane protein essential for flagellar rotation inEscherichia coli. Biochemistry. 34:1995b;9166-9171.
49. Sockett H., Yamaguchi S., Kihara M., Irikura V., Macnab R. M. Molecular analysis of the flagellar switch protein FliM of Salmonella typhimurium. J. Bacteriol. 174:1992;793-806.
50. Stader J., Matsumura P., Vacante D., Dean G. E., Macnab R. M. Nucleotide sequence of the Escherichia coli MotB gene and site-limited incorporation of its product into the cytoplasmic membrane. J. Bacteriol. 166:1986;244-252.
51. Stolz B., Berg H. C. Evidence for interactions between MotA and MotB, torque-generating elements of the flagellar motor of Escherichia coli. J. Bacteriol. 173:1991;7033-7037.
52. +-driven flagellar motors of alkalophilicBacillus. J. Biol. Chem. 263:1988;8215-8219.
53. Tang H., Braun T. F., Blair D. F. Motility protein complexes in the bacterial flagellar motor. J. Mol. Biol. 261:1996;209-221.
54. +channel function. J. Biol. Chem. 270:1995;11735-11737.
55. White B. A. PCR protocols. Methods in Molecular Biology. 1993;Humana Press, Inc. New Jersey.
56. +-driven flagellar motors of Vibrio alginolyticus. J. Biol. Chem. 265:1990;20346-20350.
57. Zhou J., Blair D. F. Residues of the cytoplasmic domain of MotA essential for torque generation in the bacterial flagellar motor. J. Mol. Biol. 273:1997;428-439.
58. Zhou J., Fazzio R. T., Blair D. F. Membrane topology of the MotA protein of Escherichia coli. J. Mol. Biol. 251:1995;237-242.
59. Zhou J., Lloyd S. A., Blair D. F. Electrostatic interactions between rotor and stator in the bacterial flagellar motor. Proc. Natl Acad. Sci. USA. 95:1998a;6436-6441.
60. Zhou J., Sharp L. L., Tang H. L., Lloyd S. A., Billings S., Braun T. F., Blair D. F. Function of protonatable residues in the flagellar motor of Escherichia coli: a critical role for Asp32 of MotB. J. Bacteriol. 180:1998b;2729-2735.