Torque generation in the flagellar motor of Escherichia coli: Evidence of a direct role for FliG but not for FliM or FliN

Journal of Bacteriology

Volumn 178, Issue 1, 1996, Pages 223-231

  Lloyd, Scott A ^a   Tang, Hua ^a   Wang, Xun ^a   Billings, Stephanie ^a   Blair, David F ^a  

^a UNIVERSITY OF UTAH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; ISOPROPYL THIOGALACTOSIDE; MUTANT PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ESCHERICHIA COLI; FLAGELLUM; MOVEMENT (PHYSIOLOGY); NONHUMAN; PARALYSIS; PHENOTYPE; PRIORITY JOURNAL; SALMONELLA TYPHIMURIUM; SITE DIRECTED MUTAGENESIS; TORQUE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0030066344     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.1.223-231.1996     Document Type: Article

References (40)

1. Blair, D. F. 1995 How bacteria sense and swim. Annu Rev Microbiol 49:489-522.
2. Blair, D. F. Unpublished data.
3. Blair, D. F., and H. C. Berg. 1990. The MotA protein of E. coli is a proton-conducting component of the flagellar motor. Cell 60:439-449.
4. Blair, D. F., and H. C. Berg. 1991. Mutations in the MotA protein of Escherichia coli reveal domains critical for proton conduction. J. Mol. Biol 221:1433-1442
5. Blair, D. F., D. Y. Kim, and H. C. Berg. 1991. Mutant MotB proteins in Escherichia coli J. Bacteriol. 173:4049-4055
6. Chun, S. Y., and J. S. Parkinson. 1988. Bacterial motility: membrane topology of the Escherichia coli MotB protein Science 239:276-278.
7. Doering, D. S. 1992. Functional and structural studies of a small F-actin binding domain. Ph.D. thesis Massachusetts Institute of Technology, Cambridge.
8. Enomoto, M. 1966. Genetic studies of paralyzed mutants in Salmonella II. Mapping of three mot loci by linkage analysis. Genetics 54:1069-1076.
9. Francis, N. R., V. M. Irikura, S. Yamaguchi, D. J. DeRosier, and R. M. Macnab. 1992. Localization of the Salmonella typhimurium flagellar switch protein FliG to the cyloplasmic M-ring face of the basal body. Proc Natl. Acad. Sci. USA 89:6304-6308
10. Francis, N. R., G. E. Sosinsky, D. Thomas, and D. J. DeRosier. 1994 Isolation, characterization and structure of bacterial flagellar motors containing the switch complex. J. Mol. Biol 235:1261-1270
11. Garza, A. G., L. W. Harris-Haller, R. A. Stoebner, and M. D. Manson. 1995. Motility protein interactions in the bacterial flagellar motor. Proc. Natl. Acad. Sci. USA 92:1970-1974.
12. Hamilton, C. M., M. Aldea, B. K. Washburn, P. Babitzke, and S. R. Kushner. 1989 New method for generating deletions and gene replacements in Escherichia coli. J. Bacteriol 171:4617-4622.
13. Heimbrook, M. E., W. L. L. Wang, and G. Campbell. 1986. Easily made flagella stains, abstr R-22, p 240. In Abstracts of the 86th Annual Meeting of the American Society for Microbiology 1986 American Society for Microbiology, Washington, D.C
14. Hilmen, M., and M. Simon. 1976. Motility and the structure of bacterial flagella, p 35-45. In R Goldman, T. Pollard, and J. Rosenbaum (ed.), Cell motility. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
15. +-driven flagellar motors of an alkalophilic Bacillus strain YN-1. J. Biol. Chem. 258:10577-10581.
16. Irikura, V. M., M. Kihara, S. Yamaguchi, H. Sockett, and R. M. Macnab. 1993. Salmonella typhimurium fliG and fliN mutations causing defects in assembly, rotation, and switching of the flagellar motor. J. Bacteriol. 175: 802-810.
17. Khan, I. H., T. S. Reese, and S. Khan. 1992 The cytoplasmic component of the bacterial flagellar motor. Proc Natl. Acad Sci. USA 89:5956-5960.
18. Khan, S., M. Dapice, and T. S. Reese. 1988. Effects of mot gene expression on the structure of the flagellar motor. J. Mol Biol 202:575-584.
19. Khan, S., I. H. Khan, and T. S. Reese. 1991. New structural features of the flagellar base in Salmonella typhimurium revealed by rapid-freeze electron microscopy J. Bacteriol. 173:2888-2896.
20. Larsen, S. H., J. Adler, J. J. Gargus, and R. W. Hogg. 1974. Chemomechanical coupling without ATP: the source of energy for motility and chemotaxis in bacteria. Proc. Natl Acad. Sci. USA 71:1239-1243.
21. Lloyd, S. A., and D. F. Blair. Unpublished data.
22. Macnab, R. 1992. Genetics and biogenesis of bacterial flagella. Annu Rev. Genet. 26:129-156.
23. Manson, M. D., P. Tedesco, H. C. Berg, F. M. Harold, and C. van der Drift. 1977. A protonmotive force drives bacterial flagella. Proc. Natl. Acad. Sci. USA 74:3060-3064.
24. Mitchell, S., S. Slechta, and D. F. Blair. Unpublished data.
25. Morrison, T. B., and J. S. Parkinson. 1994. Liberation of an interaction domain from the phosphotransfer region of CheA. a signaling kinase of Escherichia coli. Proc. Natl. Acad. Sci USA 91:5485-5489.
26. Oosawa, K., T. Ueno, and S.-I. Aizawa. 1994. Overproduction of the bacterial flagellar switch proteins and their interactions with the MS ring complex in vitro. J. Bacteriol. 176:3683-3691.
27. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989 Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
28. Schuster, S. C., and S. Khan. 1994. The bacterial flagellar motor. Annu Rev. Biophys. Biomol. Struct 23:509-539.
29. Sockett, H., S. Yamaguchi, M. Kihara, V. M. Irikura, and R. M. Macnab. 1992 Molecular analysis of the flagellar switch protein FliM of Salmonella typhimurium. J. Bacteriol. 174:793-806.
30. Stolz, B., and H. C. Berg. 1991 Evidence for interactions between MotA and MotB, torque-generating elements of the flagellar motor of Escherichia coli. J. Bacteriol. 173:7033-7037
31. Studier, F. W., and B. A. Moffatt. 1986 Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes J. Mol. Biol 189:113-130.
32. Tang, H., S. Billings, X. Wang, L. Sharp, and D. F. Blair. 1995. Regulated underexpression and overexpression of the FliN protein of Escherichia coli and evidence for an interaction between FliN and FliM in the flagellar motor. J. Bacteriol. 177:3496-3503.
33. Tang, H., and D. F. Blair. 1995. Regulated underexpression of the FliM protein in Escherichia coli and evidence for a location in the flagellar motor distinct from the MotA/MotB torque generators J. Bacteriol. 177:3485-3495.
34. Tang, H., and D. F. Blair. Unpublished data.
35. Ueno, T., K. Oosawa, and S.-I. Aizawa. 1994. Domain structures of the MS ring component protein (FliF) of the flagellar basal body of Salmonella typhimurium. J. Mol. Biol. 236:546-555.
36. Welch, M., K. Oosawa, S.-I. Aizawa, and M. Eisenbach. 1993. Phosphorylation-dependent binding of a signal molecule to the flagellar switch of bacteria. Proc Natl. Acad Sci. USA 90:8787-8791.
37. Wilson, M. L., and R. M. Macnab. 1990 Co-overproduction and localization of the Escherichia coli motility proteins MotA and MotB. J. Bacteriol. 172: 3932-3939.
38. Yamaguchi, S., S.-I. Aizawa, M. Kihara, M. Isomura, C. J. Jones, and R. M. Macnab. 1986. Genetic evidence for a switching and energy-transducing complex in the flagellar motor of Salmonella typhimurium. J. Bacteriol 168:1172-1179.
39. Yamaguchi, S., H. Fujita, A. Ishihara, S.-I. Aizawa, and R. M. Macnab. 1986 Subdivision of flagellar genes of Salmonella typhimurium into regions responsible for assembly, rotation, and switching. J. Bacteriol. 166:187-193.
40. Zhao, R., S. C. Schuster, and S. Khan. 1995. Structural effects of mutations in Salmonella typhimurium flagellar switch complex. J. Mol. Biol 251:400-412.