Identification of amino acid residues in the α, β, and γ subunits of the epithelial sodium channel (ENaC) involved in amiloride block and ion permeation

Journal of General Physiology

Volumn 109, Issue 1, 1997, Pages 15-26

  Schild, Laurent ^a   Schneeberger, Estelle ^a   Gautschi, Ivan ^a   Firsov, Dmitri ^a  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

Author keywords

amiloride; channel pore; ENaC; epithelial sodium channel; ion permeation

Indexed keywords

AMILORIDE; CYSTEINE; GLYCINE; LITHIUM CHLORIDE; POTASSIUM CHLORIDE; SODIUM CHANNEL; SODIUM CHANNEL BLOCKING AGENT; ZINC;

ALPHA CHAIN; AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; BETA CHAIN; CONCENTRATION RESPONSE; CONTROLLED STUDY; EPITHELIUM; INHIBITION KINETICS; ION PERMEABILITY; NONHUMAN; OOCYTE; RAT; XENOPUS;

AMILORIDE; AMINO ACID SEQUENCE; AMINO ACIDS; ANIMALS; CATIONS, DIVALENT; EPITHELIUM; FEMALE; IONS; OOCYTES; PERMEABILITY; RATS; SODIUM CHANNELS; XENOPUS;

EID: 0031017054     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.109.1.15     Document Type: Article

References (26)

1. Canessa, C.M., A.-M. Merillat, and B.C. Rossier. 1994a. Membrane topology of the epithelial sodium channel in intact cells. Am. J. Physiol. 267:C1682-C1690.
2. + channel is made of three homologous subunits. Nature (Lond.). 367:463-467.
3. + channel revealed by cysteine mutagenesis. Neuron. 16:1037-1047.
4. Corey, D.P. and J. García-Añoveros. 1996. Mechanosensation and the DEG/ENaC ion channels. Science (Wash. DC). 273:323-324.
5. + exchanger gene (NHE1) and amplification of the mutated allele confer amiloride resistance upon chronic acidosis. Proc. Natl Acad. Sci. USA. 90:4508-4512.
6. Favre, I., E. Moczydlowski, and L. Schild. 1995. Specificity for block by saxitoxin and divalent cations at a residue which determines sensitivity of sodium channel subtypes to guanidinium toxins. J. Gen. Physiol. 106:203-229.
7. Firsov, D., L. Schild, I. Gautschi, A.-M. Merillat, E. Schneebeiger, and B.C. Rossier. 1996. Cell surface expression of the epithelial Na channel and a mutant causing Liddle's syndrome: a quantitative approach. Proc. Natl. Acad. Sci. USA. 93:In press.
8. Guy, H.R., and S.R. Durell. 1995. Structural model of ion selective regions of epithelial sodium channels. Biophys. J. 68:A243
9. Hille, B. 1992. Ionic Channel of Excitable Membranes. Sinauer Associates, Sunderland, MA.
10. Kieber-Emmons, T., C.M. Lin, K.V. Prammer, A. Villalobos, F. KoSkiri, and T.R. Kleyman. 1995. Defining topological similarities among ion transport proteins with anti-amiloride antibodies. Kidney Int. 48:956-964.
11. Li, J.H.-Y., E.J. Cragoe, Jr., and B. Lindemann. 1987. Structure-activity relationship of amiloride analogs as blockers of epithelial Na channels: II. Side-chain modifications. J. Membr. Biol. 95:171-185.
12. MacKinnon, R. 1995. Pore loops: an emerging theme in ion channel structure. Neuron. 14:889-892.
13. North, R.A. 1996. Families of ion channels with two hydrophobic segments. Curr. Opin. Cell Biol. 8:474-483.
14. Palmer, L.G. 1985. Interactions of amiloride and other blocking cations with the apical Na channel in the toad urinary bladder. J. Membr. Biol. 87:191-199.
15. Palmer, L.G. 1990. Epithelial Na channels: the nature of the conducting pore. Renal Physiol. Biochem. 13:51-58.
16. Palmer, L.G., and O.S. Andersen. 1989. Interactions of amiloride and small monovalent cations with the epithelial sodium channel. Inferences about the nature of the channel pore. Biophys. J. 55:779-787.
17. Palmer, L.G., and G. Frinclt. 1986. Amiloride-sensitive Na channels from the apical membrane of the rat cortical collecting tubule. Proc. Natl. Acad. Sci. USA. 83:2727-2770.
18. Palmer, L.G., and G. Frindt. 1996. Gating of Na channels in the rat cortical collecting tubule: effects of voltage and membrane stretch. J. Gen. Physiol. 107:35-45.
19. + channel. J. Biol. Chem. 269:12981-12986.
20. Rossier, B.C., C.M. Canessa, L. Schild, and J.-D. Horisberger. 1994. Epithelial sodium channels. Curr. Opin. Nephrol. Hypertens. 3:487-496.
21. Schild, L., C.M. Canessa, R.A. Shimkets, I. Gautschi, R.P. Lifton, and B.C. Rossier. 1995. A mutation in the epithelial sodium channel causing Liddle disease increases channel activity in the Xenopus laevis oocyte expression system. Proc. Natl. Acad. Sci. USA. 92:5699-5703.
22. Schild, L., Y. Lu, I. Gautschi, E. Schneeberger, R.P. Lifton, and B.C. Rossier. 1996. Identification of a PY motif in the epithelial Na channel subunits as a target sequence for mutations causing channel activation found in Liddle syndrome. EMBO (Eur. Mol. Biol. Organ.) J. 15:2381-2387.
23. Snyder, P.M., F.J. McDonald, J.B. Stokes, and M.J. Welsh. 1994. Membrane topology of the amiloride-sensitive epithelial sodium channel. J. Biol. Chem. 269:24379-24383.
24. + channel. J. Biol. Chem. 270:27411-27414.
25. + channel function. J. Biol. Chem. 270: 11735-11737.
26. Woodhull, A. 1973. Ionic blockage of sodium channels in nerve. J. Gen. Physiol. 61:687-708.