Vibrio alginolyticus mutants resistant to phenamil, a specific inhibitor of the sodium-driven flagellar motor

Journal of Molecular Biology

Volumn 265, Issue 3, 1997, Pages 310-318

  Kojima, Seiji ^a   Atsumi, Tatsuo ^b   Muramoto, Kazumasa ^a,d   Kudo, Seishi ^c   Kawagishi, Ikuro ^a   Homma, Michio ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b SUZUKA NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

^c YASKAWA ELECTRIC CORPORATION   (Japan)

^d YALE UNIVERSITY   (United States)

Author keywords

Amiloride; Flagella; Motor; Sodium motive force; Vibrio

Indexed keywords

AMILORIDE DERIVATIVE; BENZAMIL; PHENAMIL; SODIUM CHANNEL; SODIUM ION;

ARTICLE; CONTROLLED STUDY; DISSOCIATION CONSTANT; DRUG RESISTANCE; FLAGELLUM; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; VIBRIO ALGINOLYTICUS;

VIBRIO; VIBRIO ALGINOLYTICUS;

EID: 0031053516     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0732     Document Type: Article

References (33)

1. Allen R. D., Baumann P. Structure and arrangement of flagella in species of the genus Beneckea and Photobacterium fischeri. J. Bacteriol. 107:1971;295-302.
2. +-driven flagellar motors of alkalophilic Bacillus strains by the amiloride analog phenamil. J. Bacteriol. 172:1990;1634-1639.
3. Atsumi T., McCarter L., Imae Y. Polar and lateral flagellar motors of marine Vibrio are driven by different ion-motive forces. Nature. 355:1992a;182-184.
4. +-driven flagellar motors of Vibrio alginolyticus but allows the cell growth. FEBS Letters. 314:1992b;114-116.
5. Atsumi T., Maekawa Y., Yamada T., Kawagishi I., Imae Y., Homma M. Effect of viscosity on swimming by the lateral and polar flagella of Vibrio alginolyticus. J. Bacteriol. 178:1996;5024-5026.
6. Blair D. F. How bacteria sense and swim. Annu. Rev. Microbiol. 49:1995;489-522.
7. Blair D. F., Berg H. C. Restoration of torque in defective flagellar motors. Science. 242:1988;1678-1681.
8. Block S. M., Berg H. C. Successive incorporation of force-generating units in the bacterial rotary motor. Nature. 309:1984;470-472.
9. +exchanger gene (NHE1) and amplification of the mutated allele confer amiloride resistance upon chronic acidosis. Proc. Natl Acad. Sci. USA. 90:1993;4508-4512.
10. Cragoe E. J. Jr, Woltersdorf O. W. Jr., Bicking J. B., Kwong S. F., Jones J. H. Pyrazine diuretics. II. N-amidino-3-amino-5-substituted 6-halopyrazinecarboxamides. J. Med. Chem. 10:1967;66-75.
11. Cragoe E. J. Jr, Kleyman T. R., Simchowitz L. Amiloride and its Analogs: 1992;VCH Publishers, Inc. New York.
12. Follett E. A. C., Gordon J. An electron microscope study of Vibrio flagella. J. Gen. Microbiol. 32:1963;235-239.
13. Homma M., Oota H., Kojima S., Kawagishi I., Imae Y. Chemotactic responses to an attractant and a repellent in the flagellar systems of Vibrio alginolyticus. Microbiology. 146:1996;2777-2783.
14. +in energy transduction. New Era of Bioenergetics. 1991;Academic Press, Inc. Tokyo.
15. +-driven bacterial flagellar motors. J. Bioenerg. Biomemb. 21:1989;705-716.
16. Kami-ike N., Kudo S., Hotani H. Rapid changes in flagellar rotation induced by external electric pulses. Biophys. J. 60:1991;1350-1355.
17. Kara-Ivanov M., Eisenbach M., Caplan S. R. Fluctuations in rotation rate of the flagellar motor of Escherichia coli. Biophys. J. 69:1995;250-263.
18. Kawagishi I., Maekawa Y., Atsumi T., Homma M., Imae Y. Isolation of the polar and lateral flagellum-defective mutants in Vibrio alginolyticus and identification of their flagellar driving energy sources. J. Bacteriol. 177:1995;5158-5160.
19. Kawagishi I., Imagawa M., Imae Y., McCarter L., Homma M. The sodium-driven polar flagellar motor of marine Vibrio as the mechanosensor that regulates lateral flagellar expression. Mol. Microbiol. 20:1996;693-699.
20. Kudo S., Magariyama Y., Aizawa S.-I. Abrupt changes in flagellar rotation observed by laser dark-field microscopy. Nature. 346:1990;677-680.
21. Li X. J., Xu R. H., Guggino W. B., Snyder S. H. Alternatively spliced forms of the α subunit of the epithelial sodium channel: distinct sites for amiloride binding and channel pore. Mol. Pharmacol. 47:1995;1133-1140.
22. Lin C., Kieber-Emmons T., Villalobos A. P., Foster M. H., Wahlgren C., Kleyman T. R. Topology of an amiloride-binding protein. J. Biol. Chem. 269:1994;2805-2813.
23. Magariyama Y., Sugiyama S., Muramoto K., Maekawa Y., Kawagishi I., Imae Y., Kudo S. Very fast flagellar rotation. Nature. 371:1994;752.
24. Magariyama Y., Sugiyama S., Muramoto K., Kawagishi I., Imae Y., Kudo S. Simultaneous measurement of bacterial flagellar rotation rate and swimming speed. Biophys. J. 69:1995;2154-2162.
25. McCarter L. L. MotY, a component of the sodium-type flagellar motor. J. Bacteriol. 176:1994a;4219-4225.
26. McCarter L. L. MotX, the channel component of the sodium-type flagellar motor. J. Bacteriol. 176:1994b;5988-5998.
27. McCarter L., Hilmen M., Silverman M. Flagellar dynamometer controls swarmet cell differentiation of V. parahaemolyticus. Cells. 54:1988;343-351.
28. Muramoto K., Sugiyama S., Cragoe E. J. Jr, Imae Y. Successive inactivation of the force-generating units of sodium-driven bacterial flagellar motors by a photoreactive amiloride analog. J. Biol. Chem. 269:1994;3374-3380.
29. Muramoto K., Kawagishi I., Kudo S., Magariyama Y., Imae Y., Homma M. High speed rota-tion and speed stability of the sodium-driven flagellar motor in Vibrio alginolyticus. J. Mol. Biol. 251:1995;50-58.
30. Muramoto K., Magariyama Y., Homma M., Kawagishi I., Sugiyama S., Imae Y., Kudo S. Rotational fluctuation of the sodium-driven flagellar motor of Vibrio alginolyticus induced by binding of inhibitors. J. Mol. Biol. 259:1996;687-695.
31. Okunishi I., Kawagishi I., Homma M. Cloning and characterization of motY, a gene coding for a component of the sodium-driven flagellar motor inVibrio alginolyticus. J. Bacteriol. 178:1996;2409-2415.
32. +-driven flagellar motors of alkalophilicBacillus. J. Biol. Chem. 263:1988;8215-8219.
33. +pump in bioenergetics of Vibrio alginolyticus. J. Biochem. 103:1988;650-655.