Targeted site-specific gas-phase cleavage of oligoribonucleotides. Application in mass spectrometry-based identification of ligand binding sites

Journal of the American Chemical Society

Volumn 121, Issue 2, 1999, Pages 474-475

  Griffey, Richard H ^a   Greig, Michael J ^a,b   An, Haoyun ^a   Sasmor, Henri ^a   Manalili, Sherilynn ^a  

^a ISIS PHARMACEUTICALS   (United States)

^b Alanex Corporation   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGORIBONUCLEOTIDE;

ARTICLE; BINDING SITE; CONFORMATIONAL TRANSITION; COVALENT BOND; DISSOCIATION; GENETIC TRANSCRIPTION; GROWTH INHIBITION; LIGAND BINDING; MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; RNA TRANSLATION; STRUCTURE ANALYSIS;

EID: 0033585581     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja983131x     Document Type: Article

References (22)

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15. 4OAc.
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18. D values are ∼0.2 μM: (a) Recht, M. I.; Fourmy, D.; Blanchard, S. C.; Dahlquist, K. D.; Puglisi, J. D.; J. Mol. Biol. 1996, 262, 421-436. (b) Wong, C. H.; Hendrix, M.; Priestley, E. S.; Greenberg, W. A.; Chem. Biol. 1998, 5, 397-406. (c) Wang, Y.; Hamasaki, Y.; Rando, R. R.; Biochemistry, 1997, 36, 768-779.
19. pp to minimize the intensity of the parent ion and to generate the highest abundance of fragment ions. A total of 128 scans were summed over m/z 700-2700 following trapping for 100 ms.
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