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10244274340
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note
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III site of pCK7. An Spel site was engineered between L1466 and Q2891 so that the DNA sequence at the fusion is CTGACTAGTCAG.
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11
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10244266616
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13C NMR coupling patterns consistent with the derivation of 5 from four intact propionate units.
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14
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10244267786
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note
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A 10% NOE interaction was observed between H-2 and H-5.
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17
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0001924338
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10244260680
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note
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12 gave 6 labeled at C-3, C-5, and C-7. The presence of only three enriched carbons in 6 is consistent with its formation by decarboxylation of the full-length acyclic tetraketide, followed by hemiketal formation between the C(7)-OH and C-3.
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-
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20
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10244233155
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note
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+ calcd 184.1463, found 184.1464.
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21
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0023885132
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