Selection of phage-displayed Fab antibodies on the active conformation of Ras yields a high affinity conformation-specific antibody preventing the binding of c-Raf kinase to Ras

FEBS Letters

Volumn 463, Issue 1-2, 1999, Pages 115-120

  Horn, Ivo R ^a   Wittinghofer, Alfred ^b   De Bruïne, Adriaan P ^a,c   Hoogenboom, Hennie R ^c  

^a MAASTRICHT UNIVERSITY MEDICAL CENTER   (Netherlands)

^b MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^c MAASTRICHT UNIVERSITY   (Netherlands)

Author keywords

Anti GTP Ras antibody; Antibody selection; c Raf kinase; GTPase activity; Phage display; Ras conformation; Surface plasmon resonance analysis

Indexed keywords

GUANOSINE TRIPHOSPHATASE; IMMUNOGLOBULIN F(AB) FRAGMENT; PROTEIN KINASE; RAF PROTEIN; RAS PROTEIN;

ANTIBODY SPECIFICITY; ARTICLE; BINDING SITE; CANCER THERAPY; CELL GROWTH; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BINDING; GENE MUTATION; GENE THERAPY; PRIORITY JOURNAL; PROTEIN CONFORMATION; SIGNAL TRANSDUCTION; SURFACE PLASMON RESONANCE;

ANTIBODIES; ANTIBODY SPECIFICITY; BINDING, COMPETITIVE; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; GTP PHOSPHOHYDROLASES; GUANOSINE DIPHOSPHATE; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; PEPTIDE LIBRARY; PROTEIN BINDING; PROTEIN CONFORMATION; PROTO-ONCOGENE PROTEINS C-RAF; RAS PROTEINS; SURFACE PLASMON RESONANCE; TIME FACTORS;

EID: 0033406045     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(99)01617-8     Document Type: Article

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