Kinase-deficient Pak1 mutants inhibit Ras transformation of Rat-1 fibroblasts

Molecular and Cellular Biology

Volumn 17, Issue 8, 1997, Pages 4454-4464

  Tang, Y I ^a   Chen, Zunxuan ^a,b   Ambrose, Diane ^c   Liu, Jianhua ^a   Gibbs, Jackson B ^b   Chernoff, Jonathan ^c   Field, Jeffrey ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b FOX CHASE CANCER CENTER   (United States)

^c MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN SERINE THREONINE KINASE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL TRANSFORMATION; ENZYME DEFICIENCY; ENZYME MECHANISM; FIBROBLAST; GENE MUTATION; INHIBITION KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

ANIMALIA;

EID: 0030811528     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.17.8.4454     Document Type: Article

References (58)

1. Al-Alawi, N., D. W. Rose, C. Buckmaster, N. Ahn, U. Rapp, J. Meinkoth, and J. R. Feramisco. 1995. Thyrotropin-induced mitogenesis is Ras dependent hut appears to bypass the Raf-dependent cytoplasmic kinase cascade. Mol. Cell. Biol. 15:1162-1168.
2. Bagrodia, S., B. Derijard, R. J. Davis, and R. A. Cerione. 1995. Cdc42 and Pak-mediated signaling leads to Jun kinase and p38 mitogen-activated protein kinase activation. J. Biol. Chem. 270:27995-27998.
3. Cdc42/Rac activated kinase. J. Biol. Chem. 270: 22731-22737.
4. Barbacid, M. 1987. ras genes. Annu. Rev. Bioehem. 56:779-827.
5. Bar-Sagi, D., and J. R. Feramisco. 1986. Induction of membrane ruffling and fluid-phase pinocytosis in quiescent fibroblasts by ras proteins. Science 233: 1061-1068.
6. Boguski, M. S., and F. McCormick. 1993. Proteins regulating Ras and its relatives. Nature 366:64.3-654.
7. Brtva, T. R., J. K. Drugan, S. Ghosh, R. S. Terrell, S. Campbell-Burk, R. M. Bell, and C. J. Der. 1995. Two distinct Raf domains mediate interaction with Ras. J. Biol. Chem. 270:9809-9812.
8. Burbelo, P. D., D. Drechsel, and A. Hall. 1995. A conserved binding motif defines numerous candidate target proteins for both Cdc42 and Rac GTPases. J. Biol. Chem. 270:29071-29074.
9. Clark, G. J., J. K. Westwick, and C. J. Der. 1997. p 120 GAP modulates Ras activation of Jun kinases and transformation. J. Biol. Chem. 272:1677-1681.
10. Coso, O. A., M. Chiariello, J.-C. Yu, H. Teramoto, P. Crespo, N. Xu, T. Miki, and J. S. Gutkind. 1995. The small GTP-binding proteins Rac1 and Cdc42 regulate the activity of the JNK/SAPK signaling pathway. Cell 81:1137-1146.
11. Cox, A. D., and C. J. Der. 1994. Biological assays for cellular transformation. Methods Enzymol. 238:277-294.
12. Deng, W. P., and J. A. Nickoloff. 1992. Site-directed mutagcnesis of virtually any plasmid by eliminating a unique site. Anal. Biochem. 200:81-88.
13. Diekmann, D., C. D. Notes, P. D. Burbelo, A. Abo, and A. Hall. 1995. Rac GTPase interacts with GAPs and target proteins through multiple effector sites. EMBO J. 14:5297-5305.
14. Drugan, J. K., R. Khosravi-Far, M. A. White, C. J. Der, Y.-J. Sung, Y.-W. Hwang, and S. L. Campbell. 1996. Ras interaction with two distinct binding domains in Raf-1 may be required for Ras transformation. J. Biol. Chem. 271:233-237.
15. Egan, S. E., and R. A. Weinberg. 1993. The pathway to signal achievement. Nature 365:781-783.
16. Frost, J. A., S. Xu, M. R. Hutchison, S. Marcus, and M. H. Cobb. 1996. Actions of Rho family small G proteins and p21 activated protein kinases on mitogen-activated protein kinase family members. Mol. Cell. Biol. 16:3707-3713
17. Galisteo, M. L., J. Chernoff, Y.-C. Su, E. Y. Skolnik, and J. Schlessinger. 1996. The adaptor protein Nck links receptor tyrosine kinases with the serine-threonine kinase Pakl. J. Biol. Chem. 271:20997-21000.
18. Ginty, D. D., A. Bonni, and M. E. Greenherg. 1994. Nerve growth factor activates a Ras-dependent protein kinase that stimulates c-fos transcription via phosphorylalion of CREB. Cell 77:713-725.
19. Hall, A. 1994. Small GTP-binding proteins and the regulation of the actin cvtoskeleton. Annu. Rev. Cell Biol. 10:31-54.
20. Han, L., and J. Colicelli. 1995. A human protein selected for interference with Ras function interacts directly with Ras and competes with Raf1. Mol. Cell. Biol. 15:1318-1323.
21. Hibi, M., A. Lin, T. Smeal, A. Minden, and M. Karin. 1993. Identification of an oncoprotein- and UV-responsive protein kinase that binds and potentiates the c-Jun activation domain. Genes Dev. 7:2135-2148.
22. Hill C. S., and R. Triesman. 1995. Transcriptional regulation by extracellular signals: mechanisms and specificity. Cell 80:199-211.
23. Hofer, F., S. Fields, C. Schneider, and G. S. Martin. 1994. Activated Ras interacts with the Ral guanine nucleotide dissociation stimulator. Proc. Natl. Acad. Sci. USA 91:11089-11093.
24. Hu, C.-D., K.-I. Kariya, M. Tamada, K. Akasaka, M. Shirouzu, S. Yokoyama, and T. Kataoka. 1995. Cysteine-rich region of Raf-l interacts with activator domain of post-translationally modified Ha-Ras. J. Biol. Chem. 270:30274-30277.
25. Joneson, T., M. McDonough, D. Bar-Sagi, and L. Van Aelst. 1996. RAC regulation of actin polymerization and proliferation by a pathway distict from Jun kinase. Science 274:1374-1376.
26. 2h. Joneson, T., M. A. White, M. H. Wigler, and D. Bar-Sagi. 1996. Stimulation of membrane ruffling and MAP kinase activation by distinct effectors of RAS. Science 271:810-812.
27. Khosravi-Kar, R., M. A. White, J. K. Westwick, P. A. Solski, M. Chrzanowska-Wodnicka, L. Van Aelst, M. H. Wigler, and C. J. Der. 1996. Oncogenic Ras activation of Raf mitogen-activated protein kinase-independent pathways is sufficient to cause tumorigenic transformation. Mol. Cell. Biol. 16:3923-3933.
28. Kohl, N. E., S. U. Mosser, S. J. deSolms, E. A. Giuliani, D. L. Pompliano, S. L. Graham, R. L. Smith, E. M. Scolnick, A. Oliff, and J. B. Gibbs. 1993. Selective inhibition of ras-dependent transformation by n farnesyltransferase inhibitor. Science 260:1934-1937.
29. PAK and the JNK/SAPK MAP kinase cascade. Cell 67:519-529.
30. Leberer, E., D. Dignard, D. Harcus, D. V. Thomas, and M. Whiteway. 1992. The protein kinase homologue Ste20p is required to link the yeast phermone response G-protein Bγ subunits to downstream signaling components. EMBO 11:4815-4824.
31. Leeuw, T., A. Fourest-Lieuvin, C. Wu, J. Chenevert, K. Clark, M. Whiteway, D. Y. Thomas, and E. Leberer. 1995. Pheromone response in yeast: association of Bem 1p with proteins of the MAP kinase cascade and actin. Science 270:1210-1212.
32. Lim, L., E. Manser, T. Leung, and C. Hall. 1996. Regulation of phosphorylation by p21 GTPases. The p21 Ras-related Rho subfamily and its role in phosphorylation signalling pathways. Eur. J. Biochem. 242:171-185.
33. Lowy, D. R., and B. R. Willumsen. 1993. Function and regulation of ras. Annu. Rev. Biochem. 62:851-891.
34. Lu, W., S. Katz, R. Gupta, and B. J. Mayer. 1997. Activation of Pak by membrane localization mediated by an SH3 domain from the adaptor protein Nck. Curr. Biol. 7:85-94.
35. Manser, E., T. Leung, H. Salihuddin, Z.-S. Zhao, and L. Lim. 1994. A brain serine/threonine protein kinase activated hy Cdc42 and Rac1. Nature 367: 40-46.
36. PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe. Proc. Natl. Acad. Sci. USA 92:6180-6184.
37. Marshall, C. J. 1995. Specificity of receptor tyrosine kinase signaling: transient versus sustained extracellular signal-regulated kinase activation. Cell 80:179-185.
38. Marshall, M. S. 1995. Ras target proteins in eukaryotic cells. FASEB J. 9:1311-1318.
39. Minden, A., A. Lin, F.-X. Claret, A. Abo, and M. Karin. 1995. Selective activation of the JNK signaling cascade and c-Jun transcriptional activity by the small GTPases Rac and Cdc42Hs. Cell 81:1147-1157.
40. Minden, A., A. Lin, M. McMahon, C. Lange-Carter, B. Derijard, R. J. Davis, G. L. Johnson, and M. Karin. 1994. Differential activation of ERK and JNK mitogen-activated protein kinases by Raf-1 and MEKK. Science 266:1719-1723.
41. Nobes, C. D., and A. Hall. 1995. Rho, Rac, and Cdc42 GTPases regulate the assembly of nultimolecular focal complexes associated with actin stress fibers, lamellipodia. and filopodia. Cell 81:53-62.
42. Oldham, S. M., G. J. Clark, L. M. Gangarosa, R. J. Coffey, and C. J. Der. 1996. Activation of the Raf-1/Map kinase cascade is not sufficient for Ras transformation of RIE-1 epithelial cells. Proc. Natl. Acad. Sci. USA 93:6924-6928.
43. + encodes a protein kinase that interacts with Cdc42p and is involved in the control of cell polarity and mating. EMBO J. 14:5908-5919.
44. Peden, K. W. C., C. Charles, L. Sanders, and G. I. Tennekoon. 1989. Isolation of rat Schwann cell lines: use of SV40 T antigen gene regulated by synthetic matallothionein promoters. Exp. Cell Res. 185:60-72.
45. Polverino, A., J. Frost, P. Yang, M. Hutchison, A. M. Neiman, M. H. Cobb, and S. Marcus. 1995. Activation of mitogen-activated protein kinase cascades by p21-activated protein kinases in cell-free extracts of Xenopus oo-cytes. J. Biol. Chem. 270:26067-26070.
46. Prendergast, G. C., R. Khosravi-Kar, P. A. Solski, H. Kurzawa, P. F. Lebowitz, and C. J. Der. 1995. Critical role of Rho in cell transformation by oncogenic Ras. Oncogene 10:2289-2296.
47. Qiu, R.-G., J. Chen, D. Kirn, F. McCorniick, and M. Symons. 1995 An essential role for Rac in Ras transformation. Nature 374:457-459.
48. Quilliam, L. A., R. Khosravi-Far, S. Y. Huff, and C. J. Der. 1995. Guanine nucleotide exchange factors; activators of the Ras superfamily of proteins. BioEssays 17:395-404.
49. Ramer, S. W., and R. W. Davis. 1993. A dominant truncation allele identifies a gene. STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 90:452-456.
50. Rodriguez-Viciana, P., P. H. Warne, R. Dhand, B. Vanhaesebroeck, I. Gout, M. J. Fry, M. D. Waterfield, and J. Downward. 1994. Phoshatidylinositol-3-OH kinase as a direct target of Ras. Nature 370:527-532.
51. Sells, M. A., and J. Chernoff. 1997. Emerging from the Pak: p21-activated protein kinases. Trends Cell Biol. 7:162-167.
52. Sells, M. A., U. G. Knaus, S. Bagrodia, D. M. Ambrose, G. M. Bokoch, and J. Chernoff. 1997. Human p21-activated kinase (Pak 1) regulates actin organization in mammalian cells. Curr. Biol. 7:202-210.
53. Settleman, J., V. Narasimhan, L. C. Foster, and R. A. Weinberg. 1992. Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from Ras to the nucleus. Cell 69:539-549.
54. Van Aelst, L., M. Barr, S. Marcus, A. Polverino, and M. H. Wigler. 1993. Complex formation between RAS and RAF and other protein kinases. Proc. Natl. Acad. Sci. USA 90:6213-6217.
55. Westwick, J. K., Q. T. Lambert, G. J. Clark, M. Symons, L. Van Aelst, R. G. Pestell, and C. J. Der. 1997. Rac regulation of transformation, gene expression, and actin organization by multiple, PAK-independent pathways. Mol Cell. Biol. 17:1324-1335.
56. Van, M., T. Dal, J. C. Deak, J. M. Kyriakis, L. I. Zon, J. R. Woodgett, and D. J. Templeton. 1994. Activation of stress-activated protein kinase by MEKK1 phosphorylation of its activator SEK1. Nature 372:798-800.
57. Zhang, S., J. Han, M. A. Sells, J. Chernoff, U. G. Knaus, R. J. Ulevitch, and G. M. Bokoch. 1995. Rho family GTPases regulate p38 mitogen-activated protein kinase through the downstream mediator Pak1. J. Biol. Chem. 270: 23934-23936.
58. Zhang, X.-F., J. Settleman, J. M. Kyriakis, E. Takeuchi-Suzuki, S. J. Elledge, M. S. Marshall, J. T. Bruder, U. R. Rapp, and J. Avruch. 1993. Normal and oncogenic p21ras proteins bind to the amino-terminal regulatory domain of c-Raf-1. Nature 364:308-313.