메뉴 건너뛰기




Volumn 16, Issue 1, 1999, Pages 190-201

High-level expression and purification of the recombinant diphtheria fusion toxin DTGM for PHASE I clinical trials

Author keywords

[No Author keywords available]

Indexed keywords

ABACA; AFFINITY CHROMATOGRAPHY; BLOOD CELL; CLINICAL TRIAL; DIPHTHERIA TOXIN; GEL PERMEATION CHROMATOGRAPHY; GENETIC ENGINEERING; HISTOCHEMISTRY; HYBRID PROTEIN; IMMUNOANALYSIS; ION EXCHANGE CHROMATOGRAPHY; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; RECOMBINANT PROTEIN;

EID: 0033152021     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1999.1071     Document Type: Article
Times cited : (36)

References (39)
  • 2
    • 0030722602 scopus 로고    scopus 로고
    • Long-term survival in acute myeloid leukemia: The Eastern Cooperative Oncology Group experience
    • Bennett, J. M., Young, M. L., Andersen, J. W., Cassileth, P. A., Tallman, M. S., Paietta, E., Wiernik, P. H., and Rowe, J. M. (1997) Long-term survival in acute myeloid leukemia: The Eastern Cooperative Oncology Group experience. Cancer 80(Suppl 11), 2205-2209.
    • (1997) Cancer , vol.80 , Issue.SUPPL. 11 , pp. 2205-2209
    • Bennett, J.M.1    Young, M.L.2    Andersen, J.W.3    Cassileth, P.A.4    Tallman, M.S.5    Paietta, E.6    Wiernik, P.H.7    Rowe, J.M.8
  • 4
    • 0030058445 scopus 로고    scopus 로고
    • Role of multidrug resistance and its pharmacological modulation in acute myeloid leukemia
    • List, A. (1996) Role of multidrug resistance and its pharmacological modulation in acute myeloid leukemia. Leukemia 10, 937-942.
    • (1996) Leukemia , vol.10 , pp. 937-942
    • List, A.1
  • 5
    • 0029867233 scopus 로고    scopus 로고
    • New chemotherapeutic agents in acute myeloid leukemia
    • Kantarjian, H. M., Estey, E. H., and Keating, M. A. (1996) New chemotherapeutic agents in acute myeloid leukemia. Leukemia 10(Suppl 1), S4-S6.
    • (1996) Leukemia , vol.10 , Issue.SUPPL. 1
    • Kantarjian, H.M.1    Estey, E.H.2    Keating, M.A.3
  • 7
    • 0025177334 scopus 로고
    • Regulation of surface expression of the granulocyte/macrophage colony-stimulating factor in normal human myeloid cells
    • Cannistra, S. A., Groshek, P., Garlick, R., Miller, J., and Griffin, J. D. (1990) Regulation of surface expression of the granulocyte/macrophage colony-stimulating factor in normal human myeloid cells. Proc. Natl. Acad. Sci. USA 87, 93-97.
    • (1990) Proc. Natl. Acad. Sci. USA , vol.87 , pp. 93-97
    • Cannistra, S.A.1    Groshek, P.2    Garlick, R.3    Miller, J.4    Griffin, J.D.5
  • 8
    • 0026409127 scopus 로고
    • Resistance of myeloid leukaemia cell lines to ricin A-chain immunotoxins
    • Engert, A., Brown, A., and Thorpe, P. (1991) Resistance of myeloid leukaemia cell lines to ricin A-chain immunotoxins. Leukemia Res. 15, 1079-1086.
    • (1991) Leukemia Res. , vol.15 , pp. 1079-1086
    • Engert, A.1    Brown, A.2    Thorpe, P.3
  • 10
    • 0026747170 scopus 로고
    • Expression cloning of a diphtheria toxin receptor: Identity with a heparin-binding EGF-like growth factor precursor
    • Naglich, J., Metherall, J., Russell, D., and Eidels, L. (1992) Expression cloning of a diphtheria toxin receptor: Identity with a heparin-binding EGF-like growth factor precursor. Cell 69, 1051-1061.
    • (1992) Cell , vol.69 , pp. 1051-1061
    • Naglich, J.1    Metherall, J.2    Russell, D.3    Eidels, L.4
  • 11
    • 0028872463 scopus 로고
    • Proteolytic activation of bacterial toxins by eukaryotic cells is performed by furin and by additional cellular proteases
    • Gordon, V., Klimpel, K., Arora, N., Henderson, M., and Leppla, S. (1995) Proteolytic activation of bacterial toxins by eukaryotic cells is performed by furin and by additional cellular proteases. Infect Immun. 63, 82-87.
    • (1995) Infect Immun. , vol.63 , pp. 82-87
    • Gordon, V.1    Klimpel, K.2    Arora, N.3    Henderson, M.4    Leppla, S.5
  • 12
    • 0019333247 scopus 로고
    • ADP-ribosylation of elongation factor 2 by diphtheria toxin: Isolation and properties of the novel ribosyl-amino acid and its hydrolysis products
    • Van Ness, B. G., Howard, J. B., and Bodley, J. W. (1980) ADP-ribosylation of elongation factor 2 by diphtheria toxin: Isolation and properties of the novel ribosyl-amino acid and its hydrolysis products. J. Biol. Chem. 255, 10717-10720.
    • (1980) J. Biol. Chem. , vol.255 , pp. 10717-10720
    • Van Ness, B.G.1    Howard, J.B.2    Bodley, J.W.3
  • 13
    • 0027265538 scopus 로고
    • DNA fragmentation and cytolysis in U937 cells treated with diphtheria toxin or other inhibitors of protein synthesis
    • Kochi, S. K., and Collier, R. J. (1993) DNA fragmentation and cytolysis in U937 cells treated with diphtheria toxin or other inhibitors of protein synthesis. Exp. Cell Res. 208, 296-302.
    • (1993) Exp. Cell Res. , vol.208 , pp. 296-302
    • Kochi, S.K.1    Collier, R.J.2
  • 14
    • 0008530328 scopus 로고
    • Genetic construction, expression, and melanoma-selective cytotoxicity of a diphtheria toxin-related alpha-melanocyte-stimulating hormone fusion protein
    • Murphy, J. R., Bishai, W., Borowski, M., Miyanohara, A., Boyd, J., and Nagle, S. (1986) Genetic construction, expression, and melanoma-selective cytotoxicity of a diphtheria toxin-related alpha-melanocyte-stimulating hormone fusion protein. Proc. Natl. Acad. Sci. USA 83, 8258-8262.
    • (1986) Proc. Natl. Acad. Sci. USA , vol.83 , pp. 8258-8262
    • Murphy, J.R.1    Bishai, W.2    Borowski, M.3    Miyanohara, A.4    Boyd, J.5    Nagle, S.6
  • 15
    • 0023529699 scopus 로고
    • Diphtheria toxin receptor binding domain substitution with interleukin-2: Genetic construction and properties of a diphtheria toxin-related interleukin-2 fusion protein
    • Williams, D. P., Parker, K., Bacha, P., Bishai, W., Borowski, M., Genbauffe, F., Strom, T. B., and Murphy, J. R. (1987) Diphtheria toxin receptor binding domain substitution with interleukin-2: Genetic construction and properties of a diphtheria toxin-related interleukin-2 fusion protein. Protein Eng. 1, 493-498.
    • (1987) Protein Eng. , vol.1 , pp. 493-498
    • Williams, D.P.1    Parker, K.2    Bacha, P.3    Bishai, W.4    Borowski, M.5    Genbauffe, F.6    Strom, T.B.7    Murphy, J.R.8
  • 16
    • 0025745412 scopus 로고
    • Interleukin 4 receptor targeted cytotoxicity: Genetic construction and in vivo immunosuppressive activity of a diphtheria toxin-related murine interleukin 4 fusion protein
    • Lakkis, F., Steele, A., Pacheco-Silva, A., Rubin-Kelley, V., Strom, T. B., and Murphy, J. R. (1991) Interleukin 4 receptor targeted cytotoxicity: Genetic construction and in vivo immunosuppressive activity of a diphtheria toxin-related murine interleukin 4 fusion protein. Eur. J. Immunol. 21, 2253-2258.
    • (1991) Eur. J. Immunol. , vol.21 , pp. 2253-2258
    • Lakkis, F.1    Steele, A.2    Pacheco-Silva, A.3    Rubin-Kelley, V.4    Strom, T.B.5    Murphy, J.R.6
  • 17
    • 0026328791 scopus 로고
    • Diphtheria toxin receptor-binding domain substitution with interleukin 6: Genetic construction and interleukin 6 receptor-specific action of a diphtheria toxin-related interleukin 6 fusion protein
    • Jean, L. F., and Murphy, J. R. (1991) Diphtheria toxin receptor-binding domain substitution with interleukin 6: Genetic construction and interleukin 6 receptor-specific action of a diphtheria toxin-related interleukin 6 fusion protein. Protein Eng. 4, 989-994.
    • (1991) Protein Eng. , vol.4 , pp. 989-994
    • Jean, L.F.1    Murphy, J.R.2
  • 18
    • 0032478339 scopus 로고    scopus 로고
    • Cytotoxicity and specificity of directed toxins composed of diphtheria toxin and the EGF-like domain of heregulin β1
    • Landgraf, R., Pegram, M., Slamon, D. J., and Eisenberg, D. (1998) Cytotoxicity and specificity of directed toxins composed of diphtheria toxin and the EGF-like domain of heregulin β1. Biochemistry 37, 3220-3228.
    • (1998) Biochemistry , vol.37 , pp. 3220-3228
    • Landgraf, R.1    Pegram, M.2    Slamon, D.J.3    Eisenberg, D.4
  • 19
    • 20244379085 scopus 로고    scopus 로고
    • Malignant progenitors from patients with acute myelogenous leukemia are sensitive to a diphtheria toxin-granulocyte-macrophage colony-stimulating factor fusion protein
    • Hogge, D. F., Willman, C. L., Kreitman, R. J., Berger, M., Hall, P. D., Kopecky, K. J., McLain, C., Tagge, E. P., Eaves, C. J., and Frankel, A. E. (1998) Malignant progenitors from patients with acute myelogenous leukemia are sensitive to a diphtheria toxin-granulocyte-macrophage colony-stimulating factor fusion protein. Blood 92, 589-595.
    • (1998) Blood , vol.92 , pp. 589-595
    • Hogge, D.F.1    Willman, C.L.2    Kreitman, R.J.3    Berger, M.4    Hall, P.D.5    Kopecky, K.J.6    McLain, C.7    Tagge, E.P.8    Eaves, C.J.9    Frankel, A.E.10
  • 20
    • 0032400865 scopus 로고    scopus 로고
    • Diphtheria toxin fused to granulocyte-macrophage colony-stimulating factor is toxic to blasts from patients with juvenile myelomonocytic leukemia and chronic myelomonocytic leukemia
    • Frankel, A. E., Lilly, M., Kreitman, R. J., Hogge, D., Beran, M., Freedman, M. H., Emanuel, P. D., McLain, C., Hall, P. D., Tagge, E. P., Berger, M., and Eaves, C. (1998) Diphtheria toxin fused to granulocyte-macrophage colony-stimulating factor is toxic to blasts from patients with juvenile myelomonocytic leukemia and chronic myelomonocytic leukemia. Blood 92, 4279-4286.
    • (1998) Blood , vol.92 , pp. 4279-4286
    • Frankel, A.E.1    Lilly, M.2    Kreitman, R.J.3    Hogge, D.4    Beran, M.5    Freedman, M.H.6    Emanuel, P.D.7    McLain, C.8    Hall, P.D.9    Tagge, E.P.10    Berger, M.11    Eaves, C.12
  • 21
    • 0030921452 scopus 로고    scopus 로고
    • Recombinant toxins containing human granulocyte-macrophage colony-stimulating factor and either pseudomonas exotoxin or diphtheria toxin kill gastrointestinal cancer and leukemia cells
    • Kreitman, R. J., and Pastan, I. (1997) Recombinant toxins containing human granulocyte-macrophage colony-stimulating factor and either pseudomonas exotoxin or diphtheria toxin kill gastrointestinal cancer and leukemia cells. Blood 90, 252-259.
    • (1997) Blood , vol.90 , pp. 252-259
    • Kreitman, R.J.1    Pastan, I.2
  • 22
    • 0030886711 scopus 로고    scopus 로고
    • Ricin fusion toxin targeted to the human granulocyte-macrophage colony-stimulating factor receptor is selectively toxic to acute myeloid leukemia cells
    • Burbage, C., Tagge, E. P., Harris, B., Hall, P. D., Fu, T., Willingham, M. C., and Frankel, A. E. (1997) Ricin fusion toxin targeted to the human granulocyte-macrophage colony-stimulating factor receptor is selectively toxic to acute myeloid leukemia cells. Leukemia Res. 21, 681-690.
    • (1997) Leukemia Res. , vol.21 , pp. 681-690
    • Burbage, C.1    Tagge, E.P.2    Harris, B.3    Hall, P.D.4    Fu, T.5    Willingham, M.C.6    Frankel, A.E.7
  • 23
    • 0030784767 scopus 로고    scopus 로고
    • Modulation of the apoptotic response of human myeloid leukemia cells to a diphtheria toxin granulocyte-macrophage colony-stimulating factor fusion protein
    • Frankel, A. E., Hall, P. D., Burbage, C., Vesely, J., Willingham, M. C., Bhalla, K., and Kreitman, R. J. (1997) Modulation of the apoptotic response of human myeloid leukemia cells to a diphtheria toxin granulocyte-macrophage colony-stimulating factor fusion protein. Blood 90, 3654-3661.
    • (1997) Blood , vol.90 , pp. 3654-3661
    • Frankel, A.E.1    Hall, P.D.2    Burbage, C.3    Vesely, J.4    Willingham, M.C.5    Bhalla, K.6    Kreitman, R.J.7
  • 24
    • 0032127605 scopus 로고    scopus 로고
    • Cell-specific modulation of drug resistance in acute myeloid leukemic blasts by diphtheria fusion toxin, DT388-GMCSF
    • Frankel, A. E., Hall, P. D., McLain, C., Safa, A. R., Tagge, E. P., and Kreitman, R. J. (1998) Cell-specific modulation of drug resistance in acute myeloid leukemic blasts by diphtheria fusion toxin, DT388-GMCSF. Bioconjugate Chem. 9, 490-496.
    • (1998) Bioconjugate Chem. , vol.9 , pp. 490-496
    • Frankel, A.E.1    Hall, P.D.2    McLain, C.3    Safa, A.R.4    Tagge, E.P.5    Kreitman, R.J.6
  • 26
    • 0031595964 scopus 로고    scopus 로고
    • Toxicology and pharmacokinetics of DT388-GM-CSF, a fusion toxin consisting of a truncated diphtheria toxin (DT388) linked to human granulocyte-macrophage colony-stimulating factor (GM-CSF) in C57BL/6 mice
    • Hall, P. D., Kreitman, R. J., Willingham, M. C., and Frankel, A. E. (1998) Toxicology and pharmacokinetics of DT388-GM-CSF, a fusion toxin consisting of a truncated diphtheria toxin (DT388) linked to human granulocyte-macrophage colony-stimulating factor (GM-CSF) in C57BL/6 mice. Toxicol. Appl. PharMacol. 150, 91-97.
    • (1998) Toxicol. Appl. Pharmacol. , vol.150 , pp. 91-97
    • Hall, P.D.1    Kreitman, R.J.2    Willingham, M.C.3    Frankel, A.E.4
  • 27
    • 0032588869 scopus 로고    scopus 로고
    • DT388-GM-CSF, a novel fusion toxin consisting of a truncated diphtheria toxin fused to human granulocyte-macrophage colony-stimulating factor, prolongs host survival in a SCID mouse model of acute myeloid leukemia
    • in press
    • Hall, P. D., Willingham, M. C., Kreitman, R. J., and Frankel, A. E. (1999) DT388-GM-CSF, a novel fusion toxin consisting of a truncated diphtheria toxin fused to human granulocyte-macrophage colony-stimulating factor, prolongs host survival in a SCID mouse model of acute myeloid leukemia. Leukemia, in press.
    • (1999) Leukemia
    • Hall, P.D.1    Willingham, M.C.2    Kreitman, R.J.3    Frankel, A.E.4
  • 28
    • 0023042283 scopus 로고
    • Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
    • Studier, F. W., and Moffatt, B. A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113-130.
    • (1986) J. Mol. Biol. , vol.189 , pp. 113-130
    • Studier, F.W.1    Moffatt, B.A.2
  • 29
    • 0023657391 scopus 로고
    • Functional domains of Pseudomonas exotoxin identified by deletion analysis of the gene expressed in E. Coli
    • Hwang, J., FitzGerald, D. J., Adhya, S., and Pastan, I. (1987) Functional domains of Pseudomonas exotoxin identified by deletion analysis of the gene expressed in E. Coli. Cell 48, 129-136.
    • (1987) Cell , vol.48 , pp. 129-136
    • Hwang, J.1    FitzGerald, D.J.2    Adhya, S.3    Pastan, I.4
  • 30
    • 0023368762 scopus 로고
    • Activity of a recombinant fusion protein between transforming growth factor type a and Pseudomonas toxin
    • Chaudhary, V. K., FitzGerald, D. J., Adhya, S., and Pastan, I. (1987) Activity of a recombinant fusion protein between transforming growth factor type a and Pseudomonas toxin. Proc. Natl. Acad. Sci. USA 84, 4538-4542.
    • (1987) Proc. Natl. Acad. Sci. USA , vol.84 , pp. 4538-4542
    • Chaudhary, V.K.1    FitzGerald, D.J.2    Adhya, S.3    Pastan, I.4
  • 31
    • 0025720456 scopus 로고
    • A proper amino terminus of diphtheria toxin is important for cytotoxicity
    • Chaudhary, V. K., FitzGerald, D. J., and Pastan, I. (1991) A proper amino terminus of diphtheria toxin is important for cytotoxicity. Biochem. Biophys. Res. Commun. 180, 545-551.
    • (1991) Biochem. Biophys. Res. Commun. , vol.180 , pp. 545-551
    • Chaudhary, V.K.1    FitzGerald, D.J.2    Pastan, I.3
  • 33
    • 0026059811 scopus 로고
    • Production of a pokeweed antiviral protein (PAP)-containing immunotoxin, B43-PAP, directed against the CD19 human B lineage lymphoid differentiation antigen in highly purified form for human clinical trials
    • Myers, D. E., Irvin, J. D., Smith, R. S., Kuebelbeck, V. M., and Uckun, F. M. (1991) Production of a pokeweed antiviral protein (PAP)-containing immunotoxin, B43-PAP, directed against the CD19 human B lineage lymphoid differentiation antigen in highly purified form for human clinical trials. J. Immunol. MethOds 136, 221-238.
    • (1991) J. Immunol. Methods , vol.136 , pp. 221-238
    • Myers, D.E.1    Irvin, J.D.2    Smith, R.S.3    Kuebelbeck, V.M.4    Uckun, F.M.5
  • 34
    • 0025785306 scopus 로고
    • The GLP large scale preparation of immunotoxins containing deglycosylated ricin A chain and a hindered disulfide bond
    • Ghetie, V., Thorpe, P., Ghetie, M. A., Knowles, P., Uhr, J. W., and Vitetta, E. S. (1991) The GLP large scale preparation of immunotoxins containing deglycosylated ricin A chain and a hindered disulfide bond. J. Immunol. Methods 142, 223-230.
    • (1991) J. Immunol. Methods , vol.142 , pp. 223-230
    • Ghetie, V.1    Thorpe, P.2    Ghetie, M.A.3    Knowles, P.4    Uhr, J.W.5    Vitetta, E.S.6
  • 35
    • 0030590640 scopus 로고    scopus 로고
    • Laboratory preparation of a deglycosylated ricin toxin A chain containing immunotoxin directed against a CD7 T lineage differentiation antigen for phase I human clinical studies involving T cell malignancies
    • Vallera, D. A., Burns, L. J., Frankel, A. E., Sicheneder, A. R., Gunther, R., Gajl-Peczalska, K., Pennell, C. A., and Kersey, J. H. (1996) Laboratory preparation of a deglycosylated ricin toxin A chain containing immunotoxin directed against a CD7 T lineage differentiation antigen for phase I human clinical studies involving T cell malignancies. J. Immunol. Methods 197, 69-83.
    • (1996) J. Immunol. Methods , vol.197 , pp. 69-83
    • Vallera, D.A.1    Burns, L.J.2    Frankel, A.E.3    Sicheneder, A.R.4    Gunther, R.5    Gajl-Peczalska, K.6    Pennell, C.A.7    Kersey, J.H.8
  • 36
    • 0026305697 scopus 로고
    • Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repression
    • Dubendorff, J. W., and Studier, F. W. (1991) Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repression. J. Mol. Biol. 219, 45-59.
    • (1991) J. Mol. Biol. , vol.219 , pp. 45-59
    • Dubendorff, J.W.1    Studier, F.W.2
  • 37
    • 0024825088 scopus 로고
    • High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product
    • Brinkmann, U., Mattes, R. E., and Buckel, P. (1989) High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product. Gene 85, 109-114.
    • (1989) Gene , vol.85 , pp. 109-114
    • Brinkmann, U.1    Mattes, R.E.2    Buckel, P.3
  • 38
    • 0026792807 scopus 로고
    • A method for increasing the yield of properly folded recombinant fusion proteins: Single-chain immunotoxins from renaturation of bacterial inclusion bodies
    • Buchner, J., Pastan, I., and Brinkmann, U. (1992) A method for increasing the yield of properly folded recombinant fusion proteins: Single-chain immunotoxins from renaturation of bacterial inclusion bodies. Anal. Biochem. 205, 263-270.
    • (1992) Anal. Biochem. , vol.205 , pp. 263-270
    • Buchner, J.1    Pastan, I.2    Brinkmann, U.3
  • 39
    • 0031422492 scopus 로고    scopus 로고
    • A form of anti-Tac(Fv) which is both single-chain and disulfide stabilized: Comparison with its single-chain and disulfide-stabilized homologs
    • Rajagopal, V., Pastan, I., and Kreitman, R. J. (1997) A form of anti-Tac(Fv) which is both single-chain and disulfide stabilized: Comparison with its single-chain and disulfide-stabilized homologs. Protein Eng. 10, 1453-1459.
    • (1997) Protein Eng. , vol.10 , pp. 1453-1459
    • Rajagopal, V.1    Pastan, I.2    Kreitman, R.J.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.