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Characterization of Yersinia enterocolitica yopB, yopD and yopR mutant strains with the digitonin fractionation technique suggests that YopB and YopR are not required for type III targeting. Although YopD is required, yopD mutants can be complemented with nonsecretable Gst-YopD fusion, suggesting that intrabacterial YopD, but not secreted YopD, appears to be necessary for type III targeting.
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